LOCUS BBa_K094004 3667 bp DNA linear 25-Nov-2024 DEFINITION plaLuxRpluxCIpldCheZGFPLva ACCESSION BBa_K094004 VERSION BBa_K094004.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 CDS 1092..1799 /label=cI lambda misc_feature 1800..1832 /label=LVA misc_feature 1839..1863 /label=Help:Barcodes CDS 1089..1838 /label=BBa_C0051 stem_loop 1883..1926 /label=stem_loop misc_feature 1872..1951 /label=BBa_B0010 terminator 1872..1951 /label=BBa_B0010 stem_loop 1967..1986 /label=T7 TE misc_feature 1993..1993 /label=stop polyA_site 1987..2000 /label=polya misc_feature 1960..2000 /label=BBa_B0012 terminator 1960..2000 /label=BBa_B0012 promoter 2046..2051 /label=-10 promoter 2023..2028 /label=-35 misc_feature 2033..2049 /label=OR1 misc_feature 2009..2025 /label=OR2 misc_feature 2009..2057 /label=BBa_R0051 promoter 2009..2057 /label=BBa_R0051 misc_feature 1071..2057 /label=BBa_Q04510 RBS 2066..2080 /label=RBS-1\Strong RBS 2073..2077 /label=RBS misc_feature 2066..2080 /label=BBa_B0030 RBS 2066..2080 /label=BBa_B0030 CDS 2087..2781 /label=protein CDS 2087..2781 /label=BBa_K094001 misc_feature 2794..2797 /label=conserved RBS 2790..2801 /label=BBa_B0034 CDS 2808..3530 /label=BBa_E0030 stem_loop 3550..3593 /label=stem_loop misc_feature 3539..3618 /label=BBa_B0010 terminator 3539..3618 /label=BBa_B0010 stem_loop 3634..3653 /label=T7 TE misc_feature 3660..3660 /label=stop polyA_site 3654..3667 /label=polya misc_feature 3627..3667 /label=BBa_B0012 terminator 3627..3667 /label=BBa_B0012 misc_feature 2790..3667 /label=BBa_E0430 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc 1141 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg 1201 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat 1261 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta 1321 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac 1381 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg 1441 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca 1501 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca 1561 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag 1621 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg 1681 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg 1741 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg 1801 ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat 1861 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 1921 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 1981 tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat tttacctctg 2041 gcggtgataa tggttgctac tagagattaa agaggagaaa tactagatga tgcaaccatc 2101 aatcaaacct gctgacgagc attcagctgg cgatatcatt gcgcgcatcg gcagcctgac 2161 gcgtatgctg cgcgacagtt tgcgggaact ggggctggat caggccattg ccgaagcggc 2221 ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt gtgcagatga ccgcccaggc 2281 tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg catcaggatc aaatggagaa 2341 atcagcaaaa gcgttaaccc aacgttggga tgactggttt gccgatccga ttgaccttgc 2401 cgacgcccgt gaactggtaa cagatacacg acaatttctg gcagatgtac ccgcgcatac 2461 cagctttact aacgcgcaac tgctggaaat catgatggcg caggattttc aggatctcac 2521 cgggcaggtc attaagcgga tgatggatgt cattcaggag atcgaacgcc agttgctgat 2581 ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca aaacgtgaaa accagagttt 2641 gcttaatgga cctcaggtcg ataccagcaa agccggtgtg gtagccagtc aggatcaggt 2701 ggacgatttg ttggatagtc ttggattttg agctagcaac gcaatgacgt cggaattgcc 2761 agctggggcg ccctctggta atactagaga aagaggagaa atactagatg gtgagcaagg 2821 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 2881 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 2941 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 3001 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct 3061 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3121 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3181 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3241 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3301 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3361 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 3421 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3481 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaataa tactagagcc 3541 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3601 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3661 gtttata //