LOCUS BBa_K094007 1836 bp DNA linear 23-Dec-2024 DEFINITION plaLuxRIpluxCheZGFPLva ACCESSION BBa_K094007 VERSION BBa_K094007.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 promoter 64..163 /label=promoter promoter 64..163 /label=BBa_K094002 misc_feature 172..191 /label=LuxR/HSL promoter 191..196 /label=-35 promoter 213..218 /label=-10 misc_feature 224..224 /label=start misc_feature 172..226 /label=BBa_R0062 promoter 172..226 /label=BBa_R0062 RBS 235..249 /label=RBS-1\Strong RBS 242..246 /label=RBS misc_feature 235..249 /label=BBa_B0030 RBS 235..249 /label=BBa_B0030 CDS 256..950 /label=protein CDS 256..950 /label=BBa_K094001 misc_feature 963..966 /label=conserved RBS 959..970 /label=BBa_B0034 CDS 977..1699 /label=BBa_E0030 stem_loop 1719..1762 /label=stem_loop misc_feature 1708..1787 /label=BBa_B0010 terminator 1708..1787 /label=BBa_B0010 stem_loop 1803..1822 /label=T7 TE misc_feature 1829..1829 /label=stop polyA_site 1823..1836 /label=polya misc_feature 1796..1836 /label=BBa_B0012 terminator 1796..1836 /label=BBa_B0012 misc_feature 959..1836 /label=BBa_E0430 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagctagtag cacgacaggt tcctagtaaa tatctaacac cgtgcgtgtt gactatttta 121 cctctggcgg tgataatggt tgcatgtagc taccgaattc agctactaga gacctgtagg 181 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataaatact agagattaaa 241 gaggagaaat actagatgat gcaaccatca atcaaacctg ctgacgagca ttcagctggc 301 gatatcattg cgcgcatcgg cagcctgacg cgtatgctgc gcgacagttt gcgggaactg 361 gggctggatc aggccattgc cgaagcggcg gaagccatcc ccgatgcgcg cgatcgtttg 421 tactatgttg tgcagatgac cgcccaggct gcggagcggg cgctgaacag tgttgaggcg 481 tcacaaccgc atcaggatca aatggagaaa tcagcaaaag cgttaaccca acgttgggat 541 gactggtttg ccgatccgat tgaccttgcc gacgcccgtg aactggtaac agatacacga 601 caatttctgg cagatgtacc cgcgcatacc agctttacta acgcgcaact gctggaaatc 661 atgatggcgc aggattttca ggatctcacc gggcaggtca ttaagcggat gatggatgtc 721 attcaggaga tcgaacgcca gttgctgatg gtgctgttgg aaaacatccc ggaacaggag 781 tcgcgtccaa aacgtgaaaa ccagagtttg cttaatggac ctcaggtcga taccagcaaa 841 gccggtgtgg tagccagtca ggatcaggtg gacgatttgt tggatagtct tggattttga 901 gctagcaacg caatgacgtc ggaattgcca gctggggcgc cctctggtaa tactagagaa 961 agaggagaaa tactagatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 1021 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga 1081 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc 1141 cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcaatgct tcgcccgcta 1201 ccccgaccac atgaagctgc acgacttctt caagtccgcc atgcccgaag gctacgtcca 1261 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt 1321 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 1381 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc 1441 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 1501 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct 1561 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa 1621 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 1681 cgagctgtac aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 1741 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 1801 ctggctcacc ttcgggtggg cctttctgcg tttata //