LOCUS BBa_K094008 1946 bp DNA linear 22-Nov-2024 DEFINITION first ACCESSION BBa_K094008 VERSION BBa_K094008.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 RBS 1071..1083 /label=RBS-3\Weak RBS 1077..1080 /label=RBS misc_feature 1071..1083 /label=BBa_B0032 RBS 1071..1083 /label=BBa_B0032 CDS 1090..1809 /label=GFP protein CDS 1090..1809 /label=BBa_E0040 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1801 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttata // LOCUS BBa_T9001 1946 bp DNA linear 22-Nov-2024 DEFINITION GFP and Producer Controlled by 3OC6HSL Receiver Device ACCESSION BBa_T9001 VERSION BBa_T9001.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 RBS 1071..1083 /label=RBS-3\Weak RBS 1077..1080 /label=RBS misc_feature 1071..1083 /label=BBa_B0032 RBS 1071..1083 /label=BBa_B0032 CDS 1090..1809 /label=GFP protein CDS 1090..1809 /label=BBa_E0040 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga 1081 aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1141 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1201 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1261 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1321 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1381 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1441 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1501 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1561 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1621 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1681 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1741 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1801 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttata //