##gff-version 3
##sequence-region BBa_K094016 1 4312
BBa_K094016	.	engineered_region	2632	4312	.	+	0	ID=BBa_K094150;Name=BBa_K094150
BBa_K094016	.	promoter	2632	2734	.	+	0	ID=BBa_K094120;Name=BBa_K094120;Parent=BBa_K094150
BBa_K094016	.	promoter	2632	2734	.	+	0	ID=annotation1980631;Name=pLac/ara-1;Parent=BBa_K094120
BBa_K094016	.	CDS	3568	4185	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K094150
BBa_K094016	.	engineered_region	3568	4185	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K094016	.	engineered_tag	4186	4210	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K094016	.	CDS	3568	4146	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K094016	.	tag	4147	4178	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K094016	.	ribosome_entry_site	3550	3561	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094150
BBa_K094016	.	conserved_region	3554	3557	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K094016	.	CDS	2761	3516	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K094150
BBa_K094016	.	engineered_region	2761	3516	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K094016	.	engineered_region	2761	2762	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K094016	.	CDS	2761	3510	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K094016	.	point_mutation	2934	2934	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K094016	.	point_mutation	3252	3252	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K094016	.	engineered_tag	3517	3541	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K094016	.	terminator	4219	4312	.	+	0	ID=BBa_K094013;Name=BBa_K094013;Parent=BBa_K094150
BBa_K094016	.	ribosome_entry_site	2743	2754	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094150
BBa_K094016	.	conserved_region	2747	2750	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K094016	.	engineered_region	1	2623	.	+	0	ID=BBa_K094015;Name=BBa_K094015
BBa_K094016	.	terminator	2530	2623	.	+	0	ID=BBa_K094013;Name=BBa_K094013;Parent=BBa_K094015
BBa_K094016	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094015
BBa_K094016	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K094016	.	CDS	82	1590	.	+	0	ID=BBa_K094011;Name=BBa_K094011;Parent=BBa_K094015
BBa_K094016	.	promoter	1701	1800	.	+	0	ID=BBa_K094002;Name=BBa_K094002;Parent=BBa_K094015
BBa_K094016	.	promoter	1701	1800	.	+	0	ID=annotation1985278;Name=promoter;Parent=BBa_K094002
BBa_K094016	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K094015
BBa_K094016	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K094016	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K094016	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K094016	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K094016	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K094016	.	ribosome_entry_site	1809	1820	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094015
BBa_K094016	.	conserved_region	1813	1816	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K094016	.	terminator	1599	1692	.	+	0	ID=BBa_K094013;Name=BBa_K094013;Parent=BBa_K094015
BBa_K094016	.	CDS	1827	2521	.	+	0	ID=BBa_K094100;Name=BBa_K094100;Parent=BBa_K094015
BBa_K094016	.	CDS	1827	2471	.	+	0	ID=annotation1980478;Name=cheZ;Parent=BBa_K094100
>BBa_K094016
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattcagcc
cttcaatcgccagagagatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcccggggatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gctgatcggcaaggtgttctggtcggcgcatagctgataacaattggtacctagaattgcttaaagaggagaaattaag
catggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccac
aagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggca
agctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacat
gaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggc
aactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttca
aggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagca
gaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccag
cagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaag
accccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct
gtacaagtaatactagagaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctcctgagtaggacaaatccgccgccctagactactagagctagtagcacgacaggttcctagtaaatatctaacacc
gtgcgtgttgactattttacctctggcggtgataatggttgcatgtagctaccgaattcagctactagagaaagaggag
aaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcc
tgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccga
tgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtca
caaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattg
accttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaa
cgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtc
attcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaa
accagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgattt
gttggatagtcttggattttgagctagcaacgcaatgacgtcggaattgccagctggggcgccctctggtaatactaga
gaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaa
atccgccgccctagactactagagcatagcatttttatccataagattagcggatctaacctttacaattgtgagcgct
cacaattatgatagattcaattgtgagcggataacaatttcacacagatactagagaaagaggagaaatactagatgaa
aaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgc
ttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctg
atatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctat
agtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcca
aatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcg
gaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccatt
aattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaaga
gaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgta
ctgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaatttt
aacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagag
gagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaa
gtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatga
taactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaaca
ggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaat
taagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagc
tatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaag
cgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtcta
tgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataact
ctgatagtgctagtgtagatctctactagagaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctcctgagtaggacaaatccgccgccctagac