BBa_K094016
type: ComponentDefinition
persistent identity: BBa K094016
display id: BBa_K094016
version: 1
was generated by: iGEM to SBOL conversion
description: pluxripluxcIcheZ
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: BBa_K094013
owned by: james
owned by: myers
mutable description: BBa_K094013
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BBa_K094013
star: false
creator: Liu Chenli
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K094150
component: BBa_K094015
sequence annotation: BBa_K094015
sequence annotation: BBa_K094150
sequence: https://synbiohub.org/public/igem/BBa_K094016_sequence/1

BBa_K094150
type: Component
persistent identity: component2302124
display id: component2302124
version: 1
top level: BBa_K094016
definition: BBa_K094150
access: public

BBa_K094015
type: Component
persistent identity: component2302106
display id: component2302106
version: 1
top level: BBa_K094016
definition: BBa_K094015
access: public

BBa_K094015
type: SequenceAnnotation
persistent identity: annotation2302106
display id: annotation2302106
version: 1
top level: BBa_K094016
location: https://synbiohub.org/public/igem/BBa_K094016/annotation2302106/range2302106/1
component: BBa_K094015

https://synbiohub.org/public/igem/BBa_K094016/annotation2302106/range2302106/1
type: Range
persistent identity: range2302106
display id: range2302106
version: 1
top level: BBa_K094016
direction: direction/reverse
start: 1
end: 2623
orientation: inline

BBa_K094150
type: SequenceAnnotation
persistent identity: annotation2302124
display id: annotation2302124
version: 1
top level: BBa_K094016
location: https://synbiohub.org/public/igem/BBa_K094016/annotation2302124/range2302124/1
component: BBa_K094150

https://synbiohub.org/public/igem/BBa_K094016/annotation2302124/range2302124/1
type: Range
persistent identity: range2302124
display id: range2302124
version: 1
top level: BBa_K094016
direction: direction/reverse
start: 2632
end: 4312
orientation: inline

BBa_K094015
type: ComponentDefinition
persistent identity: BBa K094015
display id: BBa_K094015
version: 1
was generated by: iGEM to SBOL conversion
description: pluxcIcheZ
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: BBa_K094013
owned by: james
owned by: myers
mutable description: BBa_K094013
discontinued: false
dominant: true
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: BBa_K094013
star: false
creator: Liu Chenli
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K094002
component: BBa_K094013
component: BBa_K094011
component: BBa_B0034
component: BBa_K094100
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K094011
sequence annotation: BBa_K094013
sequence annotation: BBa_K094002
sequence annotation: BBa_K094100
sequence: https://synbiohub.org/public/igem/BBa_K094015_sequence/1

BBa_K094002
type: Component
persistent identity: component1991573
display id: component1991573
version: 1
top level: BBa_K094015
definition: BBa_K094002
access: public

BBa_K094013
type: Component
persistent identity: component1991571
display id: component1991571
version: 1
top level: BBa_K094015
definition: BBa_K094013
access: public

BBa_K094011
type: Component
persistent identity: component1991570
display id: component1991570
version: 1
top level: BBa_K094015
definition: BBa_K094011
access: public

BBa_B0034
type: Component
persistent identity: component1991569
display id: component1991569
version: 1
top level: BBa_K094015
definition: BBa_B0034
access: public

BBa_K094013
type: Component
persistent identity: component1991578
display id: component1991578
version: 1
top level: BBa_K094015
definition: BBa_K094013
access: public

BBa_K094100
type: Component
persistent identity: component1991577
display id: component1991577
version: 1
top level: BBa_K094015
definition: BBa_K094100
access: public

BBa_B0034
type: Component
persistent identity: component1991575
display id: component1991575
version: 1
top level: BBa_K094015
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component1991564
display id: component1991564
version: 1
top level: BBa_K094015
definition: lux pR
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1991564
display id: annotation1991564
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991564/range1991564/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K094015/annotation1991564/range1991564/1
type: Range
persistent identity: range1991564
display id: range1991564
version: 1
top level: BBa_K094015
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991569
display id: annotation1991569
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991569/range1991569/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094015/annotation1991569/range1991569/1
type: Range
persistent identity: range1991569
display id: range1991569
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_K094011
type: SequenceAnnotation
persistent identity: annotation1991570
display id: annotation1991570
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991570/range1991570/1
component: BBa_K094011

https://synbiohub.org/public/igem/BBa_K094015/annotation1991570/range1991570/1
type: Range
persistent identity: range1991570
display id: range1991570
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 82
end: 1590
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1991571
display id: annotation1991571
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991571/range1991571/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094015/annotation1991571/range1991571/1
type: Range
persistent identity: range1991571
display id: range1991571
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 1599
end: 1692
orientation: inline

BBa_K094002
type: SequenceAnnotation
persistent identity: annotation1991573
display id: annotation1991573
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991573/range1991573/1
component: BBa_K094002

https://synbiohub.org/public/igem/BBa_K094015/annotation1991573/range1991573/1
type: Range
persistent identity: range1991573
display id: range1991573
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 1701
end: 1800
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1991575
display id: annotation1991575
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991575/range1991575/1
component: BBa_B0034

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type: Range
persistent identity: range1991575
display id: range1991575
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 1809
end: 1820
orientation: inline

BBa_K094100
type: SequenceAnnotation
persistent identity: annotation1991577
display id: annotation1991577
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991577/range1991577/1
component: BBa_K094100

https://synbiohub.org/public/igem/BBa_K094015/annotation1991577/range1991577/1
type: Range
persistent identity: range1991577
display id: range1991577
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 1827
end: 2521
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation1991578
display id: annotation1991578
version: 1
top level: BBa_K094015
location: https://synbiohub.org/public/igem/BBa_K094015/annotation1991578/range1991578/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094015/annotation1991578/range1991578/1
type: Range
persistent identity: range1991578
display id: range1991578
version: 1
top level: BBa_K094015
direction: direction/reverse
start: 2530
end: 2623
orientation: inline

BBa_K094150
type: ComponentDefinition
persistent identity: BBa K094150
display id: BBa_K094150
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI/ara-1-rbs-luxR-rbs-luxI-terminator
created: 2008-10-13T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: composition from quorum sensing system
owned by: james
owned by: myers
mutable description: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Liu Chenli
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_C0062
component: BBa_B0034
component: BBa_K094120
component: BBa_K094013
component: BBa_C0061
sequence annotation: BBa_K094120
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_C0061
sequence annotation: BBa_K094013
sequence: https://synbiohub.org/public/igem/BBa_K094150_sequence/1

BBa_C0062
type: Component
persistent identity: component2261723
display id: component2261723
version: 1
top level: BBa_K094150
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2261720
display id: component2261720
version: 1
top level: BBa_K094150
definition: BBa_B0034
access: public

BBa_K094120
type: Component
persistent identity: component2261718
display id: component2261718
version: 1
top level: BBa_K094150
definition: BBa_K094120
access: public

BBa_K094013
type: Component
persistent identity: component2261733
display id: component2261733
version: 1
top level: BBa_K094150
definition: BBa_K094013
access: public

BBa_C0061
type: Component
persistent identity: component2261730
display id: component2261730
version: 1
top level: BBa_K094150
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component2261728
display id: component2261728
version: 1
top level: BBa_K094150
definition: BBa_B0034
access: public

BBa_K094120
type: SequenceAnnotation
persistent identity: annotation2261718
display id: annotation2261718
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261718/range2261718/1
component: BBa_K094120

https://synbiohub.org/public/igem/BBa_K094150/annotation2261718/range2261718/1
type: Range
persistent identity: range2261718
display id: range2261718
version: 1
top level: BBa_K094150
direction: direction/reverse
start: 1
end: 103
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2261720
display id: annotation2261720
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261720/range2261720/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094150/annotation2261720/range2261720/1
type: Range
persistent identity: range2261720
display id: range2261720
version: 1
top level: BBa_K094150
direction: direction/reverse
start: 112
end: 123
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2261723
display id: annotation2261723
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261723/range2261723/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K094150/annotation2261723/range2261723/1
type: Range
persistent identity: range2261723
display id: range2261723
version: 1
top level: BBa_K094150
direction: direction/forward
start: 130
end: 885
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2261728
display id: annotation2261728
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261728/range2261728/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K094150/annotation2261728/range2261728/1
type: Range
persistent identity: range2261728
display id: range2261728
version: 1
top level: BBa_K094150
direction: direction/reverse
start: 919
end: 930
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2261730
display id: annotation2261730
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261730/range2261730/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_K094150/annotation2261730/range2261730/1
type: Range
persistent identity: range2261730
display id: range2261730
version: 1
top level: BBa_K094150
direction: direction/forward
start: 937
end: 1554
orientation: inline

BBa_K094013
type: SequenceAnnotation
persistent identity: annotation2261733
display id: annotation2261733
version: 1
top level: BBa_K094150
location: https://synbiohub.org/public/igem/BBa_K094150/annotation2261733/range2261733/1
component: BBa_K094013

https://synbiohub.org/public/igem/BBa_K094150/annotation2261733/range2261733/1
type: Range
persistent identity: range2261733
display id: range2261733
version: 1
top level: BBa_K094150
direction: direction/reverse
start: 1588
end: 1681
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_K094002
type: ComponentDefinition
persistent identity: BBa K094002
display id: BBa_K094002
version: 1
was generated by: iGEM to SBOL conversion
description: plambda P(O-R12)
created: 2008-07-01T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: just for testing
owned by: james
owned by: myers
mutable description: just for testing
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: just for testing
star: false
creator: Shi Lei
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: promoter
sequence: https://synbiohub.org/public/igem/BBa_K094002_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation1985278
display id: annotation1985278
version: 1
top level: BBa_K094002
location: https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K094002/annotation1985278/range1985278/1
type: Range
persistent identity: range1985278
display id: range1985278
version: 1
top level: BBa_K094002
direction: direction/forward
start: 1
end: 100
orientation: inline

BBa_K094011
type: ComponentDefinition
persistent identity: BBa K094011
display id: BBa_K094011
version: 1
was generated by: iGEM to SBOL conversion
description: CI gene
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: plaluxi
owned by: james
owned by: myers
mutable description: plaluxi
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: plaluxi
star: false
creator: Shi Lei
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K094011_sequence/1

BBa_K094013
type: ComponentDefinition
persistent identity: BBa K094013
display id: BBa_K094013
version: 1
was generated by: iGEM to SBOL conversion
description: terminator
created: 2008-08-20T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: BBa_K094013
owned by: james
owned by: myers
mutable description: BBa_K094013
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2053
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: BBa_K094013
star: false
creator: Shi Lei
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_K094013_sequence/1

BBa_K094100
type: ComponentDefinition
persistent identity: BBa K094100
display id: BBa_K094100
version: 1
was generated by: iGEM to SBOL conversion
description: cheZ gene
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: The origin of the cheZ gene comes from E. Coli strain MG1655 chromosome.
owned by: james
owned by: myers
mutable description: This part contains the cheZ operon coding region without an rbs nor a terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is without promoter, rbs or terminator.
star: false
creator: Liu Chenli
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cheZ
sequence: https://synbiohub.org/public/igem/BBa_K094100_sequence/1

cheZ
type: SequenceAnnotation
persistent identity: annotation1980478
display id: annotation1980478
version: 1
top level: BBa_K094100
location: https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K094100/annotation1980478/range1980478/1
type: Range
persistent identity: range1980478
display id: range1980478
version: 1
top level: BBa_K094100
direction: direction/forward
start: 1
end: 645
orientation: inline

BBa_K094120
type: ComponentDefinition
persistent identity: BBa K094120
display id: BBa_K094120
version: 1
was generated by: iGEM to SBOL conversion
description: pLacI/ara-1
created: 2008-10-12T11:00:00Z
modified: 2015-05-08T01:08:40Z
mutable provenance: Standard pLacI/ara-1 promoter
owned by: james
owned by: myers
mutable description: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _255_
m user id: 0
owner id: 2589
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: IPTG has better effect on this promoter than arabinose
star: false
creator: Shi Lei
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: pLac/ara-1
sequence: https://synbiohub.org/public/igem/BBa_K094120_sequence/1

pLac/ara-1
type: SequenceAnnotation
persistent identity: annotation1980631
display id: annotation1980631
version: 1
top level: BBa_K094120
location: https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K094120/annotation1980631/range1980631/1
type: Range
persistent identity: range1980631
display id: range1980631
version: 1
top level: BBa_K094120
direction: direction/forward
start: 1
end: 103
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

https://synbiohub.org/public/igem/BBa_K094016_sequence/1
type: Sequence
persistent identity: BBa K094016 sequence
display id: BBa_K094016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattcagcccttcaatcgccagagagatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcccggggatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatcggcaaggtgttctggtcggcgcatagctgataacaattggtacctagaattgcttaaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagactactagagctagtagcacgacaggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtagctaccgaattcagctactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094015_sequence/1
type: Sequence
persistent identity: BBa K094015 sequence
display id: BBa_K094015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattcagcccttcaatcgccagagagatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcccggggatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatcggcaaggtgttctggtcggcgcatagctgataacaattggtacctagaattgcttaaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagactactagagctagtagcacgacaggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtagctaccgaattcagctactagagaaagaggagaaatactagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094150_sequence/1
type: Sequence
persistent identity: BBa K094150 sequence
display id: BBa_K094150_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catagcatttttatccataagattagcggatctaacctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacagatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094002_sequence/1
type: Sequence
persistent identity: BBa K094002 sequence
display id: BBa_K094002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagtagcacgacaggttcctagtaaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtagctaccgaattcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094011_sequence/1
type: Sequence
persistent identity: BBa K094011 sequence
display id: BBa_K094011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattcagcccttcaatcgccagagagatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagcccggggatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatcggcaaggtgttctggtcggcgcatagctgataacaattggtacctagaattgcttaaagaggagaaattaagcatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094013_sequence/1
type: Sequence
persistent identity: BBa K094013 sequence
display id: BBa_K094013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgccctagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094100_sequence/1
type: Sequence
persistent identity: BBa K094100 sequence
display id: BBa_K094100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttgagctagcaacgcaatgacgtcggaattgccagctggggcgccctctggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K094120_sequence/1
type: Sequence
persistent identity: BBa K094120 sequence
display id: BBa_K094120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: catagcatttttatccataagattagcggatctaacctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z