LOCUS       BBa_K094105             1690 bp    DNA     linear       26-Dec-2024
DEFINITION  plambda P(O-R12)-rbs-cheZ-rbs-mRFP1-terminator
ACCESSION   BBa_K094105
VERSION     BBa_K094105.1  
FEATURES             Location/Qualifiers
     promoter        1..100
                     /label=promoter
     promoter        1..100
                     /label=BBa_K094002
     misc_feature    113..116
                     /label=conserved
     RBS             109..120
                     /label=BBa_B0034
     CDS             127..771
                     /label=cheZ
     CDS             127..821
                     /label=BBa_K094100
     misc_feature    1..821
                     /label=BBa_K094101
     misc_feature    834..837
                     /label=conserved
     RBS             830..841
                     /label=BBa_B0034
     CDS             848..1522
                     /label=mrfp1
     misc_feature    1529..1553
                     /label=Help:Barcodes
     CDS             848..1553
                     /label=BBa_E1010
     stem_loop       1573..1616
                     /label=stem_loop
     misc_feature    1562..1641
                     /label=BBa_B0010
     terminator      1562..1641
                     /label=BBa_B0010
     stem_loop       1657..1676
                     /label=T7 TE
     misc_feature    1683..1683
                     /label=stop
     polyA_site      1677..1690
                     /label=polya
     misc_feature    1650..1690
                     /label=BBa_B0012
     terminator      1650..1690
                     /label=BBa_B0012
     misc_feature    830..1690
                     /label=BBa_K094130
ORIGIN
        1 ctagtagcac gacaggttcc tagtaaatat ctaacaccgt gcgtgttgac tattttacct
       61 ctggcggtga taatggttgc atgtagctac cgaattcagc tactagagaa agaggagaaa
      121 tactagatga tgcaaccatc aatcaaacct gctgacgagc attcagctgg cgatatcatt
      181 gcgcgcatcg gcagcctgac gcgtatgctg cgcgacagtt tgcgggaact ggggctggat
      241 caggccattg ccgaagcggc ggaagccatc cccgatgcgc gcgatcgttt gtactatgtt
      301 gtgcagatga ccgcccaggc tgcggagcgg gcgctgaaca gtgttgaggc gtcacaaccg
      361 catcaggatc aaatggagaa atcagcaaaa gcgttaaccc aacgttggga tgactggttt
      421 gccgatccga ttgaccttgc cgacgcccgt gaactggtaa cagatacacg acaatttctg
      481 gcagatgtac ccgcgcatac cagctttact aacgcgcaac tgctggaaat catgatggcg
      541 caggattttc aggatctcac cgggcaggtc attaagcgga tgatggatgt cattcaggag
      601 atcgaacgcc agttgctgat ggtgctgttg gaaaacatcc cggaacagga gtcgcgtcca
      661 aaacgtgaaa accagagttt gcttaatgga cctcaggtcg ataccagcaa agccggtgtg
      721 gtagccagtc aggatcaggt ggacgatttg ttggatagtc ttggattttg agctagcaac
      781 gcaatgacgt cggaattgcc agctggggcg ccctctggta atactagaga aagaggagaa
      841 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
      901 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      961 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
     1021 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
     1081 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
     1141 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
     1201 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
     1261 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1321 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1381 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1441 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1501 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1561 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1621 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1681 tgcgtttata
//