LOCUS       BBa_K094171             2657 bp    DNA     linear       22-Nov-2024
DEFINITION  pTetR-rbs-lacZ alpha
ACCESSION   BBa_K094171
VERSION     BBa_K094171.1  
FEATURES             Location/Qualifiers
     misc_feature    2080..2208
                     /label=BBa_B0015 (terminator)
     misc_feature    2217..2270
                     /label=BBa_R0040 (Ptet)
     promoter        1859..1887
                     /label=beta-lactamase (ampR) promoter
     misc_feature    180..862
                     /label=ColE1 origin
     CDS             957..1817
                     /label=beta-lactamase (ampicillin resistance marker)
     stem_loop       2091..2134
                     /label=rrnB T1 terminator
     misc_feature    183..802
                     /label=pBR322
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    2058..2063
                     /label=EcoRI
     misc_feature    2064..2071
                     /label=NotI
     misc_feature    2292..2297
                     /label=XbaI
     primer_bind     1940..1959
                     /label=Verification forward (VF2) primer binding site
     primer_bind     79..98
                     /label=Verification reverse (VR) primer binding site 
     misc_feature    2279..2290
                     /label=BBa_B0034 RBS
     RBS             2279..2290
                     /label=RBS
     promoter        2217..2270
                     /label=Ptet promoter
     misc_feature    1..2298
                     /label=BBa_J61003
     CDS             2305..2652
                     /label=lacZ alpha
     misc_feature    2385..2385
                     /label=T
     misc_feature    2391..2391
                     /label=A
     misc_feature    2412..2412
                     /label=C
     misc_feature    2382..2382
                     /label=C
     misc_feature    2415..2415
                     /label=G
     promoter        2330..2339
                     /label=T3 promoter
     promoter        2477..2495
                     /label=T7 promoter
     misc_feature    2503..2652
                     /label=lacZ gene fragment
     misc_feature    2305..2319
                     /label=lacZ gene fragment
     misc_feature    2305..2657
                     /label=BBa_E0033
ORIGIN
        1 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
       61 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      121 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      181 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      241 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      301 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      361 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      421 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      481 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      541 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      601 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
      661 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      721 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      781 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      841 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      901 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      961 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1021 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1081 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1141 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1201 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1261 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1321 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1381 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1441 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1501 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1561 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1621 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1681 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1741 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1801 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1861 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1921 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1981 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2041 gctaaggatg atttctggaa ttcgcggccg caactagagc caggcatcaa ataaaacgaa
     2101 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2161 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
     2221 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagagaa
     2281 agaggagaaa ttctagagta ctagatgacc atgattacgc caagcgcgca attaaccctc
     2341 actaaaggga acaaaagctg gagctccacc gcggtggcgg cagcactaga gctagtggat
     2401 cccccgggct gtagaaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     2461 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     2521 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     2581 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     2641 cgcagcctga ataataa
//