##gff-version 3 ##sequence-region BBa_K094171 1 2657 BBa_K094171 . plasmid_vector 1 2298 . + 0 ID=BBa_J61003;Name=BBa_J61003 BBa_K094171 . engineered_region 2279 2290 . + 0 ID=annotation1975755;Name=BBa_B0034 RBS;Parent=BBa_J61003 BBa_K094171 . ribosome_entry_site 2279 2290 . + 0 ID=annotation1975756;Name=RBS;Parent=BBa_J61003 BBa_K094171 . stem_loop 2091 2134 . + 0 ID=annotation1975747;Name=rrnB T1 terminator;Parent=BBa_J61003 BBa_K094171 . CDS 957 1817 . + 0 ID=annotation1892281;Name=beta-lactamase (ampicillin resistance marker);Parent=BBa_J61003 BBa_K094171 . promoter 2217 2270 . + 0 ID=annotation1975757;Name=Ptet promoter;Parent=BBa_J61003 BBa_K094171 . primer_binding_site 79 98 . + 0 ID=annotation1975754;Name=Verification reverse (VR) primer binding site ;Parent=BBa_J61003 BBa_K094171 . sequence_feature 1 21 . + 0 ID=annotation1975749;Name=BioBrick suffix;Parent=BBa_J61003 BBa_K094171 . sequence_feature 183 802 . + 0 ID=annotation1975748;Name=pBR322;Parent=BBa_J61003 BBa_K094171 . sequence_feature 180 862 . + 0 ID=annotation1892280;Name=ColE1 origin;Parent=BBa_J61003 BBa_K094171 . sequence_feature 2064 2071 . + 0 ID=annotation1975751;Name=NotI;Parent=BBa_J61003 BBa_K094171 . engineered_region 2217 2270 . + 0 ID=annotation1892278;Name=BBa_R0040 (Ptet);Parent=BBa_J61003 BBa_K094171 . sequence_feature 2292 2297 . + 0 ID=annotation1975752;Name=XbaI;Parent=BBa_J61003 BBa_K094171 . sequence_feature 2058 2063 . + 0 ID=annotation1975750;Name=EcoRI;Parent=BBa_J61003 BBa_K094171 . primer_binding_site 1940 1959 . + 0 ID=annotation1975753;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61003 BBa_K094171 . engineered_region 2080 2208 . + 0 ID=annotation1892277;Name=BBa_B0015 (terminator);Parent=BBa_J61003 BBa_K094171 . promoter 1859 1887 . + 0 ID=annotation1892279;Name=beta-lactamase (ampR) promoter;Parent=BBa_J61003 BBa_K094171 . engineered_region 2305 2657 . + 0 ID=BBa_E0033;Name=BBa_E0033 BBa_K094171 . promoter 2477 2495 . + 0 ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033 BBa_K094171 . sequence_feature 2503 2652 . + 0 ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033 BBa_K094171 . sequence_alteration 2391 2391 . + 0 ID=annotation308381;Name=A;Parent=BBa_E0033 BBa_K094171 . sequence_alteration 2382 2382 . + 0 ID=annotation308387;Name=C;Parent=BBa_E0033 BBa_K094171 . sequence_alteration 2385 2385 . + 0 ID=annotation308375;Name=T;Parent=BBa_E0033 BBa_K094171 . CDS 2305 2652 . + 0 ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033 BBa_K094171 . promoter 2330 2339 . + 0 ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033 BBa_K094171 . sequence_alteration 2412 2412 . + 0 ID=annotation308383;Name=C;Parent=BBa_E0033 BBa_K094171 . sequence_alteration 2415 2415 . + 0 ID=annotation308388;Name=G;Parent=BBa_E0033 BBa_K094171 . sequence_feature 2305 2319 . + 0 ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033 >BBa_K094171 tactagtagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagca aaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatag ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaag atcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatc aaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg ctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggga taataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatc ttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttag aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatga cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgattt ctggaattcgcggccgcaactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact agagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaat tctagagtactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccacc gcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacct cgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcg tgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataa