LOCUS BBa_K098983 3637 bp DNA linear 26-Nov-2024 DEFINITION lac inducible mtrB (low expression of QPI) ACCESSION BBa_K098983 VERSION BBa_K098983.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23113 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 stem_loop 1234..1277 /label=stem_loop misc_feature 1223..1302 /label=BBa_B0010 terminator 1223..1302 /label=BBa_B0010 stem_loop 1318..1337 /label=T7 TE misc_feature 1344..1344 /label=stop polyA_site 1338..1351 /label=polya misc_feature 1311..1351 /label=BBa_B0012 terminator 1311..1351 /label=BBa_B0012 terminator 1223..1351 /label=BBa_B0015 misc_feature 1362..1378 /label=lac O1 promoter 1379..1384 /label=-35 misc_feature 1385..1401 /label=lac O1 promoter 1402..1407 /label=-10 misc_feature 1360..1413 /label=BBa_R0011 promoter 1360..1413 /label=BBa_R0011 misc_feature 44..1414 /label=BBa_Q04121 misc_feature 1..1414 /label=BBa_K098985 misc_feature 1427..1430 /label=conserved RBS 1423..1434 /label=BBa_B0034 CDS 1441..3534 /label=mtrB CDS 1441..3534 /label=BBa_K098989 stem_loop 3550..3569 /label=T7 TE misc_feature 3576..3576 /label=stop polyA_site 3570..3583 /label=polya misc_feature 3543..3583 /label=BBa_B0012 terminator 3543..3583 /label=BBa_B0012 stem_loop 3604..3626 /label=stem_loop misc_feature 3592..3637 /label=BBa_B0011 terminator 3592..3637 /label=BBa_B0011 misc_feature 1423..3637 /label=BBa_K098994 ORIGIN 1 ctgatggcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1321 tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt 1381 gacattgtga gcggataaca agatactgag cacatactag agaaagagga gaaatactag 1441 atgaaattta aactcaattt gatcactcta gcgttattag ccaacacagg cttggccgtc 1501 gctgctgatg gttatggtct agcgaatgcc aatactgaaa aagtgaaatt atccgcatgg 1561 agctgtaaag gctgcgtcgt tgaaacgggc acatcaggca ctgtgggtgt cggtgtcggt 1621 tataacagcg aagaggatat tcgctctgcc aatgcctttg gtacatccaa tgaagtggcg 1681 ggtaaatttg atgccgattt aaactttaaa ggtgaaaagg gttatcgtgc cagtgttgat 1741 gcttatcaac tcggtatgga tggcggtcgc ttagatgtca atgcgggcaa acaaggccag 1801 tacaacgtca atgtgaacta tcgccaaatt gctacctacg acagcaatag cgccctatcg 1861 ccctacgcgg gtattggtgg caataacctc acgttaccgg ataactggat aacagcaggt 1921 tcaagcaacc aaatgccact cttgatggac agcctcaatg ccctcgaact ctcacttaaa 1981 cgtgagcgca cggggttggg atttgaatat caaggtgaat ccctgtggag cacctatgtt 2041 aactacatgc gtgaagagaa aaccggctta aaacaagcct ctggtagctt cttcaaccaa 2101 tcgatgatgt tagcagagcc ggtggattac accactgaca ccattgaagc gggtgtcaaa 2161 ctcaagggtg atcgttggtt taccgcactc agttacaatg ggtcaatatt caaaaacgaa 2221 tacaaccaat tggactttga aaatgctttt aaccccacct ttggtgctca aacccaaggt 2281 acgatggcac tcgatccgga taaccagtca cacaccgtgt cgctgatggg acagtacaac 2341 gatggcagca acgcactgtc gggtcgtatt ctgaccggac aaatgagcca agatcaggcg 2401 ttagtgacgg ataactaccg ttatgctaat cagctcaata ccgatgccgt cgatgccaaa 2461 gtcgatctac tgggtatgaa cctgaaagtc gttagcaaag tgagcaatga tcttcgctta 2521 acaggtagtt acgattatta cgaccgtgac aataataccc aagtagaaga atggactcag 2581 atcagcatca acaatgtcaa cggtaaggtg gcttataaca ccccttacga taatcgtacg 2641 caacgcttta aagttgccgc agattatcgc attacccgcg atatcaaact cgatggtggt 2701 tatgacttca aacgtgacca acgtgattat caagaccgtg aaaccacgga tgaaaatacc 2761 gtttgggccc gtttacgtgt aaacagcttc gatacttggg acatgtgggt aaaaggcagt 2821 tacggtaacc gtgacggctc acaataccaa gcgtctgaat ggacctcttc tgaaaccaac 2881 agcctgttac gtaagtacaa tctggctgac cgtgacagaa ctcaagtcga agcacggatc 2941 acccattcgc cattagaaag cctgactatc gatgttggtg cccgttacgc gttagatgat 3001 tataccgata ctgtgattgg attaactgag tcaaaagaca ccagttatga tgccaacatc 3061 agttatatga tcaccgctga cttactggca accgccttct acaattacca aaccattgag 3121 tctgaacagg cgggtagcag caattacagc accccaacgt ggacaggctt tatagaagat 3181 caggtagatg tggtcggtgc aggtatcagc tacaacaatc tgctggagaa caagttacgc 3241 ctaggactgg actacaccta ttccaactcc gacagtaaca ctcaagtcag acaaggtatc 3301 actggcgact atggtgatta ttttgccaaa gtgcataaca ttaacttata cgctcaatat 3361 caagccaccg agaaactcgc gctgcgcttc gattacaaaa ttgagaacta taaggacaat 3421 gacgccgcaa atgatatcgc cgttgatggc atttggaacg tcgtaggttt tggtagtaac 3481 agccatgact acaccgcaca aatgctgatg ctgagcatga gttacaaact ctaatactag 3541 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat 3601 aaaaagccag attattaatc cggctttttt attattt //