LOCUS       BBa_K098987             1813 bp    DNA     linear       28-Nov-2024
DEFINITION  temperature sensitive cI inducible system with GFP reporter and 
            low promoter
ACCESSION   BBa_K098987
VERSION     BBa_K098987.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             56..769
                     /label=cIts
     CDS             56..769
                     /label=BBa_K098997
     misc_feature    44..769
                     /label=BBa_K098998
     stem_loop       785..804
                     /label=T7 TE
     misc_feature    811..811
                     /label=stop
     polyA_site      805..818
                     /label=polya
     misc_feature    778..818
                     /label=BBa_B0012
     terminator      778..818
                     /label=BBa_B0012
     stem_loop       839..861
                     /label=stem_loop
     misc_feature    827..872
                     /label=BBa_B0011
     terminator      827..872
                     /label=BBa_B0011
     terminator      778..872
                     /label=BBa_B0014
     promoter        918..923
                     /label=-10
     promoter        895..900
                     /label=-35
     misc_feature    905..921
                     /label=OR1
     misc_feature    881..897
                     /label=OR2
     misc_feature    881..929
                     /label=BBa_R0051
     promoter        881..929
                     /label=BBa_R0051
     RBS             938..950
                     /label=RBS-3\Weak
     RBS             944..947
                     /label=RBS
     misc_feature    938..950
                     /label=BBa_B0032
     RBS             938..950
                     /label=BBa_B0032
     CDS             957..1676
                     /label=GFP protein
     CDS             957..1676
                     /label=BBa_E0040
     stem_loop       1696..1739
                     /label=stem_loop
     misc_feature    1685..1764
                     /label=BBa_B0010
     terminator      1685..1764
                     /label=BBa_B0010
     stem_loop       1780..1799
                     /label=T7 TE
     misc_feature    1806..1806
                     /label=stop
     polyA_site      1800..1813
                     /label=polya
     misc_feature    1773..1813
                     /label=BBa_B0012
     terminator      1773..1813
                     /label=BBa_B0012
     misc_feature    881..1813
                     /label=BBa_K098991
     misc_feature    44..1813
                     /label=BBa_S04138
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaaatgag
       61 cacaaaaaag aaaccattaa cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat
      121 ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa tctgtcgcag acaagatggg
      181 gatggggcag tcaggcgttg gtgctttatt taatggcatc aatgcattaa atgcttataa
      241 cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc
      301 cagagaaatc tacgagatgt atgaagcggt tagtatgcag ccgtcactta gaagtgagta
      361 tgagtaccct gttttttctc atgttcaggc agggatgttc tcacctgagc ttagaacctt
      421 taccaaaggt gatgcggaga gatgggtaag cacaaccaaa aaagccagtg attctgcatt
      481 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
      541 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
      601 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
      661 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
      721 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggctgat actagagtca
      781 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
      841 gccagattat taatccggct tttttattat tttactagag taacaccgtg cgtgttgact
      901 attttacctc tggcggtgat aatggttgct actagagtca cacaggaaag tactagatgc
      961 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
     1021 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
     1081 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
     1141 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
     1201 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1261 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1321 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1381 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1441 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1501 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1561 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1621 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1681 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1741 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1801 ttctgcgttt ata
//