LOCUS       BBa_K098990             2258 bp    DNA     linear       22-Nov-2024
DEFINITION  mtrB, low expression
ACCESSION   BBa_K098990
VERSION     BBa_K098990.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..2155
                     /label=mtrB
     CDS             62..2155
                     /label=BBa_K098989
     stem_loop       2171..2190
                     /label=T7 TE
     misc_feature    2197..2197
                     /label=stop
     polyA_site      2191..2204
                     /label=polya
     misc_feature    2164..2204
                     /label=BBa_B0012
     terminator      2164..2204
                     /label=BBa_B0012
     stem_loop       2225..2247
                     /label=stem_loop
     misc_feature    2213..2258
                     /label=BBa_B0011
     terminator      2213..2258
                     /label=BBa_B0011
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaattt aaactcaatt tgatcactct agcgttatta gccaacacag gcttggccgt
      121 cgctgctgat ggttatggtc tagcgaatgc caatactgaa aaagtgaaat tatccgcatg
      181 gagctgtaaa ggctgcgtcg ttgaaacggg cacatcaggc actgtgggtg tcggtgtcgg
      241 ttataacagc gaagaggata ttcgctctgc caatgccttt ggtacatcca atgaagtggc
      301 gggtaaattt gatgccgatt taaactttaa aggtgaaaag ggttatcgtg ccagtgttga
      361 tgcttatcaa ctcggtatgg atggcggtcg cttagatgtc aatgcgggca aacaaggcca
      421 gtacaacgtc aatgtgaact atcgccaaat tgctacctac gacagcaata gcgccctatc
      481 gccctacgcg ggtattggtg gcaataacct cacgttaccg gataactgga taacagcagg
      541 ttcaagcaac caaatgccac tcttgatgga cagcctcaat gccctcgaac tctcacttaa
      601 acgtgagcgc acggggttgg gatttgaata tcaaggtgaa tccctgtgga gcacctatgt
      661 taactacatg cgtgaagaga aaaccggctt aaaacaagcc tctggtagct tcttcaacca
      721 atcgatgatg ttagcagagc cggtggatta caccactgac accattgaag cgggtgtcaa
      781 actcaagggt gatcgttggt ttaccgcact cagttacaat gggtcaatat tcaaaaacga
      841 atacaaccaa ttggactttg aaaatgcttt taaccccacc tttggtgctc aaacccaagg
      901 tacgatggca ctcgatccgg ataaccagtc acacaccgtg tcgctgatgg gacagtacaa
      961 cgatggcagc aacgcactgt cgggtcgtat tctgaccgga caaatgagcc aagatcaggc
     1021 gttagtgacg gataactacc gttatgctaa tcagctcaat accgatgccg tcgatgccaa
     1081 agtcgatcta ctgggtatga acctgaaagt cgttagcaaa gtgagcaatg atcttcgctt
     1141 aacaggtagt tacgattatt acgaccgtga caataatacc caagtagaag aatggactca
     1201 gatcagcatc aacaatgtca acggtaaggt ggcttataac accccttacg ataatcgtac
     1261 gcaacgcttt aaagttgccg cagattatcg cattacccgc gatatcaaac tcgatggtgg
     1321 ttatgacttc aaacgtgacc aacgtgatta tcaagaccgt gaaaccacgg atgaaaatac
     1381 cgtttgggcc cgtttacgtg taaacagctt cgatacttgg gacatgtggg taaaaggcag
     1441 ttacggtaac cgtgacggct cacaatacca agcgtctgaa tggacctctt ctgaaaccaa
     1501 cagcctgtta cgtaagtaca atctggctga ccgtgacaga actcaagtcg aagcacggat
     1561 cacccattcg ccattagaaa gcctgactat cgatgttggt gcccgttacg cgttagatga
     1621 ttataccgat actgtgattg gattaactga gtcaaaagac accagttatg atgccaacat
     1681 cagttatatg atcaccgctg acttactggc aaccgccttc tacaattacc aaaccattga
     1741 gtctgaacag gcgggtagca gcaattacag caccccaacg tggacaggct ttatagaaga
     1801 tcaggtagat gtggtcggtg caggtatcag ctacaacaat ctgctggaga acaagttacg
     1861 cctaggactg gactacacct attccaactc cgacagtaac actcaagtca gacaaggtat
     1921 cactggcgac tatggtgatt attttgccaa agtgcataac attaacttat acgctcaata
     1981 tcaagccacc gagaaactcg cgctgcgctt cgattacaaa attgagaact ataaggacaa
     2041 tgacgccgca aatgatatcg ccgttgatgg catttggaac gtcgtaggtt ttggtagtaa
     2101 cagccatgac tacaccgcac aaatgctgat gctgagcatg agttacaaac tctaatacta
     2161 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     2221 taaaaagcca gattattaat ccggcttttt tattattt
//