BBa_K100000
1
PN
Natural Xylose Regulated Bi-Directional Operator
2008-08-17T11:00:00Z
2015-05-08T01:08:41Z
Taken from E. Coli genome bases 3728830-3729092
This part contains the operator region controlling transcription for the xyl operon. This regulatory region is dependent upon the binding of CRP-cAMP and XylR bound to xylose for activation. Upon binding, transcription is activated in both directions (xylFGH and xylAB facing). This part has an unscrambled CRP binding site and and unaltered promoter region (unlike BBa_K100001 through BBa_K100003).
false
false
_213_
0
1752
9
It's complicated
false
Both directions were kept in the sequence of the part even though it is intended to work in the right facing direction because of possible looping that may occur as a transcriptional control.
false
Garrett Tobin
annotation1972263
1
RNA poly. binding site
range1972263
1
243
248
annotation1972196
1
RNA poly. binding site
range1972196
1
45
50
annotation1972266
1
RNA poly. binding site
range1972266
1
264
269
annotation1972261
1
CRP binding site
range1972261
1
90
107
annotation1972173
1
RNA poly. binding site
range1972173
1
21
26
BBa_K100000_sequence
1
agggtgatggatgatgtcgtaatattgggcactccctttcagttgctcaattatgttatttcacactgctattgagataattcacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z