LOCUS       BBa_K100004             1187 bp    DNA     linear       27-Dec-2024
DEFINITION  Natural Xylose Regulated Bi-Directional Operator + GFP
ACCESSION   BBa_K100004
VERSION     BBa_K100004.1  
FEATURES             Location/Qualifiers
     misc_feature    21..26
                     /label=RNA poly. binding site
     misc_feature    45..50
                     /label=RNA poly. binding site
     misc_feature    90..107
                     /label=CRP binding site
     misc_feature    243..248
                     /label=RNA poly. binding site
     misc_feature    264..269
                     /label=RNA poly. binding site
     promoter        1..303
                     /label=BBa_K100000
     RBS             312..324
                     /label=RBS-3\Weak
     RBS             318..321
                     /label=RBS
     misc_feature    312..324
                     /label=BBa_B0032
     RBS             312..324
                     /label=BBa_B0032
     CDS             331..1050
                     /label=GFP protein
     CDS             331..1050
                     /label=BBa_E0040
     stem_loop       1070..1113
                     /label=stem_loop
     misc_feature    1059..1138
                     /label=BBa_B0010
     terminator      1059..1138
                     /label=BBa_B0010
     stem_loop       1154..1173
                     /label=T7 TE
     misc_feature    1180..1180
                     /label=stop
     polyA_site      1174..1187
                     /label=polya
     misc_feature    1147..1187
                     /label=BBa_B0012
     terminator      1147..1187
                     /label=BBa_B0012
ORIGIN
        1 agggtgatgg atgatgtcgt aatattgggc actccctttc agttgctcaa ttatgttatt
       61 tcacactgct attgagataa ttcacaagtg tgcgctcgct cgcaaaataa aatggaatga
      121 tgaaactggg taattcctcg aagagaaaaa tgcaataagt acaattgcgc aacaaaagta
      181 agatctcggt cataaatcaa gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt
      241 aattgttttc ccctgtttag ttgctaaaaa ttggttacgt ttatcgcggt gattgttact
      301 tattactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga
      361 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      421 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      481 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      541 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      601 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      661 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      721 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      781 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      841 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      901 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      961 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1021 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1081 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1141 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//