LOCUS       BBa_K1001755            1917 bp    DNA     linear       22-Dec-2024
DEFINITION  AraC generator then Lambda promoter controlling RFP production, 
            "Scissors"
ACCESSION   BBa_K1001755
VERSION     BBa_K1001755.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=BBa_J61127
     CDS             19..894
                     /label=araC
     misc_feature    895..927
                     /label=LVA
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             19..958
                     /label=BBa_C0080
     misc_feature    1..958
                     /label=BBa_K228032
     misc_feature    1022..1035
                     /label=OR1 434
     misc_feature    975..991
                     /label=OR1 lambda
     misc_feature    999..1015
                     /label=OR2 lambda
     promoter        1037..1042
                     /label=-10
     promoter        1014..1019
                     /label=-35
     promoter        967..1048
                     /label=BBa_I12006
     misc_feature    1061..1064
                     /label=conserved
     RBS             1057..1068
                     /label=BBa_B0034
     CDS             1075..1749
                     /label=mrfp1
     misc_feature    1756..1780
                     /label=Help:Barcodes
     CDS             1075..1780
                     /label=BBa_E1010
     misc_feature    1057..1780
                     /label=BBa_S03873
     misc_feature    967..1780
                     /label=BBa_K1001753
     stem_loop       1800..1843
                     /label=stem_loop
     misc_feature    1789..1868
                     /label=BBa_B0010
     terminator      1789..1868
                     /label=BBa_B0010
     stem_loop       1884..1903
                     /label=T7 TE
     misc_feature    1910..1910
                     /label=stop
     polyA_site      1904..1917
                     /label=polya
     misc_feature    1877..1917
                     /label=BBa_B0012
     terminator      1877..1917
                     /label=BBa_B0012
     terminator      1789..1917
                     /label=BBa_B0015
     misc_feature    967..1917
                     /label=BBa_K1001754
ORIGIN
        1 aaagagtgga actactagat ggctgaagcg caaaatgatc ccctgctgcc gggatactcg
       61 tttaacgccc atctggtggc gggtttaacg ccgattgagg ccaatggtta tctcgatttt
      121 tttatcgacc gaccgctggg aatgaaaggt tatattctca atctcaccat tcgcggtcag
      181 ggggtggtga aaaatcaggg acgagaattt gtctgccgac cgggtgatat tttgctgttc
      241 ccgccaggag agattcatca ctacggtcgt catccggagg ctcgcgaatg gtatcaccag
      301 tgggtttact ttcgtccgcg cgcctactgg catgaatggc ttaactggcc gtcaatattt
      361 gccaatacgg gtttctttcg cccggatgaa gcgcaccagc cgcatttcag cgacctgttt
      421 gggcaaatca ttaacgccgg gcaaggggaa gggcgctatt cggagctgct ggcgataaat
      481 ctgcttgagc aattgttact gcggcgcatg gaagcgatta acgagtcgct ccatccaccg
      541 atggataatc gggtacgcga ggcttgtcag tacatcagcg atcacctggc agacagcaat
      601 tttgatatcg ccagcgtcgc acagcatgtt tgcctgtcgc cgtcgcgtct gtcacatctt
      661 ttccgccagc agttagggat tagcgtctta agctggcgcg aggaccaacg catcagccag
      721 gcgaagctgc ttttgagcac tacccggatg cctatcgcca ccgtcggtcg caatgttggt
      781 tttgacgatc aactctattt ctcgcgagta tttaaaaaat gcaccggggc cagcccgagc
      841 gagttccgtg ccggttgtga agaaaaagtg aatgatgtag ccgtcaagtt gtcagctgca
      901 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta
      961 ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg tgttagatat
     1021 tacaaacttt cttgtataga tttaacgtta ctagagaaag aggagaaata ctagatggct
     1081 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
     1141 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
     1201 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
     1261 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
     1321 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
     1381 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     1441 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     1501 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     1561 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     1621 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     1681 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     1741 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
     1801 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1861 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//