"Scissors"
type: ComponentDefinition
persistent identity: BBa K1001755
display id: BBa_K1001755
version: 1
was generated by: iGEM to SBOL conversion
description: AraC generator then Lambda promoter controlling RFP production, "Scissors"
created: 2013-05-01T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: The AraC region comes from BBa_K228032. The promoter, rbs, rfp, and double terminator come from BBa_K1001754.
owned by: james
owned by: myers
mutable description: This is a project specific part. This sequence contains an AraC coding gene (BBa_K228032) upstream of a double terminator behind a Red Flourescent Protein and Ribosome Binding Site behind the modified Lambda Prm Promoter(BBa_K1001754). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _1301_
m user id: 0
owner id: 15456
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Part "Scissors"
star: false
creator: Vansh Singh
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K228032
component: BBa_K1001754
sequence annotation: BBa_K228032
sequence annotation: BBa_K1001754
sequence: https://synbiohub.org/public/igem/BBa_K1001755_sequence/1

BBa_K228032
type: Component
persistent identity: component2269381
display id: component2269381
version: 1
top level: "Scissors"
definition: BBa_K228032
access: public

BBa_K1001754
type: Component
persistent identity: component2269402
display id: component2269402
version: 1
top level: "Scissors"
definition: "Part Y"
access: public

BBa_K228032
type: SequenceAnnotation
persistent identity: annotation2269381
display id: annotation2269381
version: 1
top level: "Scissors"
location: https://synbiohub.org/public/igem/BBa_K1001755/annotation2269381/range2269381/1
component: BBa_K228032

https://synbiohub.org/public/igem/BBa_K1001755/annotation2269381/range2269381/1
type: Range
persistent identity: range2269381
display id: range2269381
version: 1
top level: "Scissors"
direction: direction/reverse
start: 1
end: 958
orientation: inline

BBa_K1001754
type: SequenceAnnotation
persistent identity: annotation2269402
display id: annotation2269402
version: 1
top level: "Scissors"
location: https://synbiohub.org/public/igem/BBa_K1001755/annotation2269402/range2269402/1
component: BBa_K1001754

https://synbiohub.org/public/igem/BBa_K1001755/annotation2269402/range2269402/1
type: Range
persistent identity: range2269402
display id: range2269402
version: 1
top level: "Scissors"
direction: direction/reverse
start: 967
end: 1917
orientation: inline

"Part Y"
type: ComponentDefinition
persistent identity: BBa K1001754
display id: BBa_K1001754
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator RFP and RBS behind Lamda Prm Promoter
created: 2013-05-01T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: This comes from BBa_K1001753 and BBa_B0015.
owned by: james
owned by: myers
mutable description: This sequence contains double terminator(BBa_B0015) behind a Red Flourescent Protein and Ribosome Binding Site behind the modified Lambda Prm Promoter(BBa_K1001753). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed. Use this BioBrick if your team wishes to have the inducible Lamda Prm promoter to control the expression of an RFP.
discontinued: false
dominant: false
experience: experience/None
group u list: _1301_
m user id: 0
owner id: 15456
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This uses the modified Lamda Prm Promoter specifically for its positive regulation.
star: false
creator: Vansh Singh
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K1001753
component: BBa_B0015
sequence annotation: BBa_K1001753
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1001754_sequence/1

BBa_K1001753
type: Component
persistent identity: component2259881
display id: component2259881
version: 1
top level: "Part Y"
definition: "Part X"
access: public

BBa_B0015
type: Component
persistent identity: component2259888
display id: component2259888
version: 1
top level: "Part Y"
definition: BBa_B0015
access: public

BBa_K1001753
type: SequenceAnnotation
persistent identity: annotation2259881
display id: annotation2259881
version: 1
top level: "Part Y"
location: https://synbiohub.org/public/igem/BBa_K1001754/annotation2259881/range2259881/1
component: BBa_K1001753

https://synbiohub.org/public/igem/BBa_K1001754/annotation2259881/range2259881/1
type: Range
persistent identity: range2259881
display id: range2259881
version: 1
top level: "Part Y"
direction: direction/reverse
start: 1
end: 814
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2259888
display id: annotation2259888
version: 1
top level: "Part Y"
location: https://synbiohub.org/public/igem/BBa_K1001754/annotation2259888/range2259888/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1001754/annotation2259888/range2259888/1
type: Range
persistent identity: range2259888
display id: range2259888
version: 1
top level: "Part Y"
direction: direction/reverse
start: 823
end: 951
orientation: inline

BBa_K228032
type: ComponentDefinition
persistent identity: BBa K228032
display id: BBa_K228032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS and AraC coding sequence
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: Partsregistry Distribution 2009
owned by: james
owned by: myers
mutable description: <partinfo>BBa_J61127</partinfo> and <partinfo>C0080</partinfo>
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J61127
component: BBa_C0080
sequence annotation: BBa_J61127
sequence annotation: BBa_C0080
sequence: https://synbiohub.org/public/igem/BBa_K228032_sequence/1

BBa_J61127
type: Component
persistent identity: component2244249
display id: component2244249
version: 1
top level: BBa_K228032
definition: BBa_J61127
access: public

BBa_C0080
type: Component
persistent identity: component2244253
display id: component2244253
version: 1
top level: BBa_K228032
definition: araC
access: public

BBa_J61127
type: SequenceAnnotation
persistent identity: annotation2244249
display id: annotation2244249
version: 1
top level: BBa_K228032
location: https://synbiohub.org/public/igem/BBa_K228032/annotation2244249/range2244249/1
component: BBa_J61127

https://synbiohub.org/public/igem/BBa_K228032/annotation2244249/range2244249/1
type: Range
persistent identity: range2244249
display id: range2244249
version: 1
top level: BBa_K228032
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2244253
display id: annotation2244253
version: 1
top level: BBa_K228032
location: https://synbiohub.org/public/igem/BBa_K228032/annotation2244253/range2244253/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_K228032/annotation2244253/range2244253/1
type: Range
persistent identity: range2244253
display id: range2244253
version: 1
top level: BBa_K228032
direction: direction/reverse
start: 19
end: 958
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

"Part X"
type: ComponentDefinition
persistent identity: BBa K1001753
display id: BBa_K1001753
version: 1
was generated by: iGEM to SBOL conversion
description: RFP and RBS behind Lamda Prm Promoter
created: 2013-05-01T11:00:00Z
modified: 2015-05-08T01:08:41Z
mutable provenance: We use the intermediate part, BBa_S03873, formed from an RFP (BBa_E1010) and an RBS (BBa_B0034).
owned by: james
owned by: myers
mutable description: This sequence contains a Red Flourescent Protein and Ribosome Binding Site (BBa_S03873) behind the modified Lambda Prm Promoter(BBa_I12006). This specific modified Lambda Promoter is induced by the lamda cl repressor (BBa_C0051) instead of repressed by it. When induced, the RFP is expressed. Use this BioBrick if your team wishes to have the inducible Lamda Prm promoter to control the expression of an RFP.
discontinued: false
dominant: false
experience: experience/None
group u list: _1301_
m user id: 0
owner id: 15456
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We specifically used the modified Lambda Prm Promoter because it was positively regulated.
star: false
creator: Vansh Singh
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S03873
component: BBa_I12006
sequence annotation: BBa_I12006
sequence annotation: BBa_S03873
sequence: https://synbiohub.org/public/igem/BBa_K1001753_sequence/1

BBa_S03873
type: Component
persistent identity: component2248339
display id: component2248339
version: 1
top level: "Part X"
definition: BBa_S03873
access: public

BBa_I12006
type: Component
persistent identity: component2248333
display id: component2248333
version: 1
top level: "Part X"
definition: Prm +
access: public

BBa_I12006
type: SequenceAnnotation
persistent identity: annotation2248333
display id: annotation2248333
version: 1
top level: "Part X"
location: https://synbiohub.org/public/igem/BBa_K1001753/annotation2248333/range2248333/1
component: BBa_I12006

https://synbiohub.org/public/igem/BBa_K1001753/annotation2248333/range2248333/1
type: Range
persistent identity: range2248333
display id: range2248333
version: 1
top level: "Part X"
direction: direction/reverse
start: 1
end: 82
orientation: inline

BBa_S03873
type: SequenceAnnotation
persistent identity: annotation2248339
display id: annotation2248339
version: 1
top level: "Part X"
location: https://synbiohub.org/public/igem/BBa_K1001753/annotation2248339/range2248339/1
component: BBa_S03873

https://synbiohub.org/public/igem/BBa_K1001753/annotation2248339/range2248339/1
type: Range
persistent identity: range2248339
display id: range2248339
version: 1
top level: "Part X"
direction: direction/reverse
start: 91
end: 814
orientation: inline

BBa_J61127
type: ComponentDefinition
persistent identity: BBa J61127
display id: BBa_J61127
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-24T11:00:00Z
modified: 2015-08-31T01:59:49Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61127_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_S03873
type: ComponentDefinition
persistent identity: BBa S03873
display id: BBa_S03873
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:E1010
created: 2007-10-25T11:00:00Z
modified: 2015-05-08T01:14:31Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _82_
m user id: 0
owner id: 927
owning group id: 82
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Kathy Lam
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_E1010
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_S03873_sequence/1

BBa_B0034
type: Component
persistent identity: component2243942
display id: component2243942
version: 1
top level: BBa_S03873
definition: BBa_B0034
access: public

BBa_E1010
type: Component
persistent identity: component2243945
display id: component2243945
version: 1
top level: BBa_S03873
definition: mRFP1
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243942
display id: annotation2243942
version: 1
top level: BBa_S03873
location: https://synbiohub.org/public/igem/BBa_S03873/annotation2243942/range2243942/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03873/annotation2243942/range2243942/1
type: Range
persistent identity: range2243942
display id: range2243942
version: 1
top level: BBa_S03873
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2243945
display id: annotation2243945
version: 1
top level: BBa_S03873
location: https://synbiohub.org/public/igem/BBa_S03873/annotation2243945/range2243945/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S03873/annotation2243945/range2243945/1
type: Range
persistent identity: range2243945
display id: range2243945
version: 1
top level: BBa_S03873
direction: direction/reverse
start: 19
end: 724
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12006
display id: BBa_I12006
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lamdba Prm promoter (repressed by 434 cI)
created: 2004-07-13T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Bushman(1993), Shih & Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lamdba Prm promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI)
discontinued: false
dominant: false
experience: experience/None
group u list: _3_
m user id: 0
owner id: 147
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The O-R1 region of 434 contained 14 base pairs as opposed to the 17 base pairs of the O-R3 site of lambda. Also, it was noticed that the O-R3 site of the lambda included part of the -10 site. Hence, to preserve the spacing and the -10 site, the three nucleotides that were in both the -10 site and the lambda O-R3 site were retained. The 14 nucleotides that were in the O-R3 site and not in the -10 site were replaced with the O-R1 site of the 434.
star: true
creator: mcnamara
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1 lambda
sequence annotation: OR2 lambda
sequence annotation: -35
sequence annotation: OR1 434
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12006_sequence/1

OR1 lambda
type: SequenceAnnotation
persistent identity: annotation786500
display id: annotation786500
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786500/range786500/1
type: Range
persistent identity: range786500
display id: range786500
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2 lambda
type: SequenceAnnotation
persistent identity: annotation786518
display id: annotation786518
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786518/range786518/1
type: Range
persistent identity: range786518
display id: range786518
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation837284
display id: annotation837284
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837284/range837284/1
type: Range
persistent identity: range837284
display id: range837284
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

OR1 434
type: SequenceAnnotation
persistent identity: annotation786365
display id: annotation786365
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12006/annotation786365/range786365/1
type: Range
persistent identity: range786365
display id: range786365
version: 1
top level: Prm +
direction: direction/forward
start: 56
end: 69
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation837228
display id: annotation837228
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12006/annotation837228/range837228/1
type: Range
persistent identity: range837228
display id: range837228
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_K1001755_sequence/1
type: Sequence
persistent identity: BBa K1001755 sequence
display id: BBa_K1001755_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagagtggaactactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1001754_sequence/1
type: Sequence
persistent identity: BBa K1001754 sequence
display id: BBa_K1001754_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228032_sequence/1
type: Sequence
persistent identity: BBa K228032 sequence
display id: BBa_K228032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagagtggaactactagatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1001753_sequence/1
type: Sequence
persistent identity: BBa K1001753 sequence
display id: BBa_K1001753_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61127_sequence/1
type: Sequence
persistent identity: BBa J61127 sequence
display id: BBa_J61127_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagagtggaac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03873_sequence/1
type: Sequence
persistent identity: BBa S03873 sequence
display id: BBa_S03873_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12006_sequence/1
type: Sequence
persistent identity: BBa I12006 sequence
display id: BBa_I12006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatattacaaactttcttgtatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z