LOCUS BBa_K1001758 1739 bp DNA linear 26-Dec-2024 DEFINITION AraC coding upstream of Lux promoter upstream of GFP ACCESSION BBa_K1001758 VERSION BBa_K1001758.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 22..729 /label=cI lambda misc_feature 730..762 /label=LVA misc_feature 769..793 /label=Help:Barcodes CDS 19..793 /label=BBa_C0051 misc_feature 1..793 /label=BBa_K093006 misc_feature 802..821 /label=LuxR/HSL promoter 821..826 /label=-35 promoter 843..848 /label=-10 misc_feature 854..854 /label=start misc_feature 802..856 /label=BBa_R0062 promoter 802..856 /label=BBa_R0062 misc_feature 869..872 /label=conserved RBS 865..876 /label=BBa_B0034 CDS 883..1602 /label=GFP protein CDS 883..1602 /label=BBa_E0040 misc_feature 865..1602 /label=BBa_I13500 misc_feature 802..1602 /label=BBa_K1001757 stem_loop 1622..1665 /label=stem_loop misc_feature 1611..1690 /label=BBa_B0010 terminator 1611..1690 /label=BBa_B0010 stem_loop 1706..1725 /label=T7 TE misc_feature 1732..1732 /label=stop polyA_site 1726..1739 /label=polya misc_feature 1699..1739 /label=BBa_B0012 terminator 1699..1739 /label=BBa_B0012 terminator 1611..1739 /label=BBa_B0015 misc_feature 802..1739 /label=BBa_S05143 ORIGIN 1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 781 tagtgtagat cgctactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt 841 gttatagtcg aataaatact agagaaagag gagaaatact agatgcgtaa aggagaagaa 901 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 961 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 1021 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 1081 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 1141 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 1201 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 1261 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 1321 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 1381 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 1441 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 1501 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 1561 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca 1621 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1681 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //