BBa_K101001
1
BBa_K101001
Dual-Repressed Promoter for LacI and LambdacI
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
false
false
_205_
0
3363
9
Not in stock
false
LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
false
Bennett Swiniarski
BBa_K101001_sequence
1
gcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggcggtgataatgtgtggaattgtgagcggataaaatttcacaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z