LOCUS       BBa_K101004              587 bp    DNA     linear       23-May-2024
DEFINITION  Construct of LacI promoter, RBS, p22 mnt gene, terminator
ACCESSION   BBa_K101004
VERSION     BBa_K101004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..476
                     /label=mnt
     misc_feature    322..324
                     /label=T
     misc_feature    477..509
                     /label=LVA
     misc_feature    516..540
                     /label=Help:Barcodes
     CDS             228..540
                     /label=BBa_C0072
     misc_feature    549..587
                     /label=B1006
     stem_loop       558..579
                     /label=modified thr terminator
     polyA_site      580..587
                     /label=PolyA
     polyA_site      549..557
                     /label=PolyA
     terminator      549..587
                     /label=BBa_B1006
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gcccgggatg
      241 atcctcactt caattttcgt atgccaatgg aagtaagaga gaaattgaaa tttagagcag
      301 aggcaaacgg acggagcatg aactctgagc ttttgcaaat cgtacaagat gccctaagca
      361 aaccgtcacc agtcactggg taccgcaatg atgcggaacg actcgccgat gagcagagcg
      421 agttagtgaa gaagatggtc ttcgatacac tgaaggatct ttataaaaaa accaccgctg
      481 caaacgacga aaactacgct ttagtagctt aataaccctg atagtgctag tgtagatccc
      541 tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttt
//