LOCUS       BBa_K101005              910 bp    DNA     linear       23-May-2024
DEFINITION  Construct with LacI/LAMBDAcI promoter, RBS, GFP, Terminator
ACCESSION   BBa_K101005
VERSION     BBa_K101005.1  
FEATURES             Location/Qualifiers
     promoter        1..116
                     /label=BBa_K101001
     RBS             125..137
                     /label=RBS-3\Weak
     RBS             131..134
                     /label=RBS
     misc_feature    125..137
                     /label=BBa_B0032
     RBS             125..137
                     /label=BBa_B0032
     CDS             144..863
                     /label=GFP protein
     CDS             144..863
                     /label=BBa_E0040
     misc_feature    872..910
                     /label=B1006
     stem_loop       881..902
                     /label=modified thr terminator
     polyA_site      903..910
                     /label=PolyA
     polyA_site      872..880
                     /label=PolyA
     terminator      872..910
                     /label=BBa_B1006
ORIGIN
        1 gcgcaacgca attaatgtga gttagctcac tcattaggca taacaccgtg cgtgttgact
       61 attttacctc tggcggtgat aatgtgtgga attgtgagcg gataaaattt cacacatact
      121 agagtcacac aggaaagtac tagatgcgta aaggagaaga acttttcact ggagttgtcc
      181 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg
      241 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac
      301 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga
      361 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      421 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca
      481 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag
      541 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca
      601 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      661 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      721 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      781 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      841 tggatgaact atacaaataa taatactaga gaaaaaaaaa ccccgcccct gacagggcgg
      901 ggtttttttt
//