BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K101001
1
BBa_K101001
Dual-Repressed Promoter for LacI and LambdacI
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
false
false
_205_
0
3363
9
Not in stock
false
LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
false
Bennett Swiniarski
BBa_K101005
1
BBa_K101005
Construct with LacI/LAMBDAcI promoter, RBS, GFP, Terminator
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
This construct is composed of: LacI promoter ( R0010), LAMBDAcI promoter (R0051), RBS (B0032), GFP (E0040), and Terminator (B1006).
Has a promoter which is dually repressed by LacI and LAMBDAcI. The RBS is the ribosomal binding site. The GFP is a green fluorescent protein, which is a reporter gene. The Terminator is used to terminate RNA polymerase's transcription.
false
false
_205_
0
3370
9
It's complicated
false
Majority of design considerations were dealt with during the design of the dually repressed promoter (K101001). A medium strength RBS was used to increase the affinity of this part for ribosomal binding. Additionally, this decision was made to use only a single terminator.
false
Ellen Martin
component1968798
1
BBa_B1006
component1968788
1
BBa_K101001
component1968793
1
BBa_E0040
component1968790
1
BBa_B0032
annotation1968793
1
BBa_E0040
range1968793
1
144
863
annotation1968788
1
BBa_K101001
range1968788
1
1
116
annotation1968798
1
BBa_B1006
range1968798
1
872
910
annotation1968790
1
BBa_B0032
range1968790
1
125
137
BBa_B1006
1
BBa_B1006
Terminator (artificial, large, %T~>90)
2006-08-30T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~>90%
*8bp stem, 6nt loop
*Bidirectional, estimated reverse %T~>90%
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
true
Haiyao Huang
annotation1898431
1
PolyA
range1898431
1
1
9
annotation1898430
1
PolyA
range1898430
1
32
39
annotation1898428
1
B1006
range1898428
1
1
39
annotation1898429
1
modified thr terminator
range1898429
1
10
31
BBa_K101005_sequence
1
gcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggcggtgataatgtgtggaattgtgagcggataaaatttcacacatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_K101001_sequence
1
gcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggcggtgataatgtgtggaattgtgagcggataaaatttcacaca
BBa_B0032_sequence
1
tcacacaggaaag
BBa_B1006_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z