LOCUS BBa_K101008 3273 bp DNA linear 22-Nov-2024 DEFINITION Biological Comparator Inducible by IPTG and aTc ACCESSION BBa_K101008 VERSION BBa_K101008.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..75 /label=RBS-3\Weak RBS 69..72 /label=RBS misc_feature 63..75 /label=BBa_B0032 RBS 63..75 /label=BBa_B0032 CDS 85..792 /label=cI lambda misc_feature 793..825 /label=LVA misc_feature 832..856 /label=Help:Barcodes CDS 82..856 /label=BBa_C0051 misc_feature 865..903 /label=B1006 stem_loop 874..895 /label=modified thr terminator polyA_site 896..903 /label=PolyA polyA_site 865..873 /label=PolyA terminator 865..903 /label=BBa_B1006 CDS 912..999 /label=end of LacI coding region (inactive) misc_feature 912..1111 /label=BBa_R0010 misc_feature 1000..1037 /label=CAP binding site promoter 1048..1053 /label=-35 promoter 1072..1077 /label=-10 misc_feature 1077..1111 /label=LacI binding site misc_feature 1084..1084 /label=start promoter 912..1111 /label=BBa_R0010 RBS 1120..1132 /label=RBS-3\Weak RBS 1126..1129 /label=RBS misc_feature 1120..1132 /label=BBa_B0032 RBS 1120..1132 /label=BBa_B0032 CDS 1139..1387 /label=mnt misc_feature 1233..1235 /label=T misc_feature 1388..1420 /label=LVA misc_feature 1427..1451 /label=Help:Barcodes CDS 1139..1451 /label=BBa_C0072 misc_feature 1460..1498 /label=B1006 stem_loop 1469..1490 /label=modified thr terminator polyA_site 1491..1498 /label=PolyA polyA_site 1460..1468 /label=PolyA terminator 1460..1498 /label=BBa_B1006 promoter 1507..1622 /label=BBa_K101001 RBS 1631..1643 /label=RBS-3\Weak RBS 1637..1640 /label=RBS misc_feature 1631..1643 /label=BBa_B0032 RBS 1631..1643 /label=BBa_B0032 CDS 1650..2369 /label=GFP protein CDS 1650..2369 /label=BBa_E0040 misc_feature 2378..2416 /label=B1006 stem_loop 2387..2408 /label=modified thr terminator polyA_site 2409..2416 /label=PolyA polyA_site 2378..2386 /label=PolyA terminator 2378..2416 /label=BBa_B1006 promoter 2425..2485 /label=BBa_K101000 RBS 2494..2506 /label=RBS-3\Weak RBS 2500..2503 /label=RBS misc_feature 2494..2506 /label=BBa_B0032 RBS 2494..2506 /label=BBa_B0032 CDS 2513..3223 /label=mCherry misc_feature 2864..2864 /label=C->T (removing PstI site) misc_feature 2513..3226 /label=BBa_J06504 misc_feature 3235..3273 /label=B1006 stem_loop 3244..3265 /label=modified thr terminator polyA_site 3266..3273 /label=PolyA polyA_site 3235..3243 /label=PolyA terminator 3235..3273 /label=BBa_B1006 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gaaagtacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 121 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 181 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 241 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 301 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 361 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 421 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 481 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 541 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 601 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 661 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 721 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 781 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 841 tgctagtgta gatcgctact agagaaaaaa aaaccccgcc cctgacaggg cggggttttt 901 ttttactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 1021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 1081 tggaattgtg agcggataac aatttcacac atactagagt cacacaggaa agtactagat 1141 ggcccgggat gatcctcact tcaattttcg tatgccaatg gaagtaagag agaaattgaa 1201 atttagagca gaggcaaacg gacggagcat gaactctgag cttttgcaaa tcgtacaaga 1261 tgccctaagc aaaccgtcac cagtcactgg gtaccgcaat gatgcggaac gactcgccga 1321 tgagcagagc gagttagtga agaagatggt cttcgataca ctgaaggatc tttataaaaa 1381 aaccaccgct gcaaacgacg aaaactacgc tttagtagct taataaccct gatagtgcta 1441 gtgtagatcc ctactagaga aaaaaaaacc ccgcccctga cagggcgggg ttttttttta 1501 ctagaggcgc aacgcaatta atgtgagtta gctcactcat taggcataac accgtgcgtg 1561 ttgactattt tacctctggc ggtgataatg tgtggaattg tgagcggata aaatttcaca 1621 catactagag tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag 1681 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg 1741 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg 1801 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct 1861 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag 1921 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg 1981 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta 2041 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat 2101 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca 2161 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg 2221 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc 2281 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac 2341 atggcatgga tgaactatac aaataataat actagagaaa aaaaaacccc gcccctgaca 2401 gggcggggtt tttttttact agagtcccta tcagtgatag agattgacaa ggtccacggt 2461 gacctagatc tccgatactg agcactacta gagtcacaca ggaaagtact agatggtgag 2521 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat 2581 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta 2641 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg 2701 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga 2761 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa 2821 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccttgcagg acggcgagtt 2881 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa 2941 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa 3001 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa 3061 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa 3121 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga 3181 gggccgccac tccaccggcg gcatggacga gctgtacaag taataatact agagaaaaaa 3241 aaaccccgcc cctgacaggg cggggttttt ttt //