##gff-version 3
##sequence-region BBa_K101008 1 3273
BBa_K101008	.	promoter	1507	1622	.	+	0	ID=BBa_K101001;Name=BBa_K101001
BBa_K101008	.	promoter	2425	2485	.	+	0	ID=BBa_K101000;Name=BBa_K101000
BBa_K101008	.	ribosome_entry_site	1631	1643	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K101008	.	ribosome_entry_site	1637	1640	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K101008	.	ribosome_entry_site	1631	1643	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K101008	.	engineered_region	1631	1643	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K101008	.	CDS	82	856	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K101008	.	engineered_tag	832	856	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K101008	.	CDS	85	792	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K101008	.	tag	793	825	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K101008	.	ribosome_entry_site	2494	2506	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K101008	.	ribosome_entry_site	2500	2503	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K101008	.	ribosome_entry_site	2494	2506	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K101008	.	engineered_region	2494	2506	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K101008	.	terminator	865	903	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K101008	.	polyA_site	865	873	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	polyA_site	896	903	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	engineered_region	865	903	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K101008	.	stem_loop	874	895	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K101008	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K101008	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K101008	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K101008	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K101008	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K101008	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K101008	.	CDS	1139	1451	.	+	0	ID=BBa_C0072;Name=BBa_C0072
BBa_K101008	.	tag	1388	1420	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K101008	.	CDS	1139	1387	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K101008	.	engineered_tag	1427	1451	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K101008	.	point_mutation	1233	1235	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K101008	.	terminator	2378	2416	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K101008	.	polyA_site	2378	2386	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	polyA_site	2409	2416	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	engineered_region	2378	2416	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K101008	.	stem_loop	2387	2408	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K101008	.	terminator	1460	1498	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K101008	.	polyA_site	1460	1468	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	polyA_site	1491	1498	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	engineered_region	1460	1498	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K101008	.	stem_loop	1469	1490	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K101008	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K101008	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K101008	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K101008	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K101008	.	engineered_region	2513	3226	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K101008	.	sequence_alteration	2864	2864	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K101008	.	CDS	2513	3223	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K101008	.	CDS	1650	2369	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K101008	.	CDS	1650	2369	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K101008	.	terminator	3235	3273	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K101008	.	polyA_site	3235	3243	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	polyA_site	3266	3273	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K101008	.	engineered_region	3235	3273	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K101008	.	stem_loop	3244	3265	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K101008	.	ribosome_entry_site	1120	1132	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K101008	.	ribosome_entry_site	1126	1129	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K101008	.	ribosome_entry_site	1120	1132	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K101008	.	engineered_region	1120	1132	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K101008	.	promoter	912	1111	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K101008	.	non_covalent_binding_site	1000	1037	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K101008	.	promoter	1072	1077	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K101008	.	promoter	1048	1053	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K101008	.	start_codon	1084	1084	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K101008	.	non_covalent_binding_site	1077	1111	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K101008	.	engineered_region	912	1111	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K101008	.	CDS	912	999	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
>BBa_K101008
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaa
gaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattt
aatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcc
cttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccc
tgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggta
agcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcca
agccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccag
acttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacag
tacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttg
gcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaaaa
aaaaccccgcccctgacagggcggggtttttttttactagagcaatacgcaaaccgcctctccccgcgcgttggccgat
tcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctc
actcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacatactagagtcacacaggaaagtactagatggcccgggatgatcctcacttcaattttcgtatgccaatggaagt
aagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaatcgtacaagatgcc
ctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagagcgagttagtgaaga
agatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactacgctttagtagctta
ataaccctgatagtgctagtgtagatccctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttac
tagaggcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggc
ggtgataatgtgtggaattgtgagcggataaaatttcacacatactagagtcacacaggaaagtactagatgcgtaaag
gagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcag
tggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcca
tggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgact
ttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacg
tgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaac
attcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatca
aagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaat
tggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaag
agagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
actagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactagagtccctatcagtgatagagattgaca
aggtccacggtgacctagatctccgatactgagcactactagagtcacacaggaaagtactagatggtgagcaagggcg
aggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagtt
cgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggcccc
ctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatcc
ccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgac
cgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgac
ggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagg
gcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaa
gcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtg
gaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaaaa
aaaaccccgcccctgacagggcggggtttttttt