BBa_K101009
1
BBa_K101009
Dual-Repressed Promoter for p22 Mnt and TetR with RBS (med strength)
2008-07-27T11:00:00Z
2015-05-08T01:08:42Z
This part combines BBa_K101000, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.
This promoter will be repressed in the presence of the Mnt repressor from the P22 phage, the Tet repressor from E. coli, or both. A medium strength RBS has been incorporated for convenient cloning to a desired gene.
false
false
_205_
0
3296
9
Not in stock
false
The dually-repressed promoter contains a TetR operator site derived from the pTet promoter of E. coli upstream of the -35 polymerase binding site, and a P22 Mnt operator site between the -35 and -10 polymerase binding sites. The -35 and -10 binding site were derived from the pTet promoter. For information concerning the RBS design, please see part BBa_B0032.
false
Kristen J Lindblad
component1968824
1
BBa_K101000
component1968826
1
BBa_B0032
annotation1968826
1
BBa_B0032
range1968826
1
70
82
annotation1968824
1
BBa_K101000
range1968824
1
1
61
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K101000
1
BBa_K101000
Dual-Repressed Promoter for p22 mnt and TetR
2008-07-06T11:00:00Z
2015-05-08T01:08:41Z
This part was designed by combining sequences from parts C0040 (TetR promoter) and C0072 (p22 mnt promoter).
This part is a promoter that is repressed by both p22 mnt and TetR. It can be used to control the response of any gene that follows this promoter. It will be repressed by the presence of either p22 mnt or TetR.
false
false
_205_
0
3363
9
Not in stock
false
TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
false
Bennett Swiniarski
BBa_K101009_sequence
1
tccctatcagtgatagagattgacaaggtccacggtgacctagatctccgatactgagcactactagagtcacacaggaaag
BBa_K101000_sequence
1
tccctatcagtgatagagattgacaaggtccacggtgacctagatctccgatactgagcac
BBa_B0032_sequence
1
tcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z