BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K101010
1
BBa_K101010
Dual-Repressed Promoter for p22 cII and TetR with RBS attached
2008-07-27T11:00:00Z
2015-05-08T01:08:42Z
This part was constructed from BioBrick parts BBa_K101002 (Dual Promoter) and BBa_B0032 (medium strength RBS).
This composite part consists of a dually-repressed promoter with a medium strength RBS attached. The promoter is repressed by either p22 cII or TetR proteins. The medium strength RBS was attached to make this promoter easier to use with any protein that may need to be attached to it. When the RBS is already on the promoter, a simpler ligation can occur that attaches the protein to this promoter, instead of having to attach a small RBS before this ligation can occur.
false
false
_205_
0
3363
9
Not in stock
false
There were no real design considerations needed for this part. All that was done was to use PCR to add the small RBS fragment to the dual promoter construct.
false
Bennett Swiniarski
component1968819
1
BBa_K101002
component1968821
1
BBa_B0032
annotation1968819
1
BBa_K101002
range1968819
1
1
66
annotation1968821
1
BBa_B0032
range1968821
1
75
87
BBa_K101002
1
BBa_K101002
Dual-Repressed Promoter for p22 cII and TetR
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
This part was designed by combining sequences from parts R0040 (TetR promoter) and R0053 (p22 cII promoter).
k;kljg
false
false
_205_
0
3363
9
Not in stock
false
TetR usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. However, p22 mnt also has two operator sites, one between -35 and -10 and another downstream from -10. Since each had an operator site in between -35 and -10, we had to eliminate the TetR operator site in order to allow the promoter to work. TetR was picked because it has a stronger binding than p22 mnt, and so would be more able to use only one site to bind.
false
Bennett Swiniarski
BBa_K101002_sequence
1
tccctatcagtgatagagattgactaaagattcctttagtagataatttaagtgttctttaatttc
BBa_K101010_sequence
1
tccctatcagtgatagagattgactaaagattcctttagtagataatttaagtgttctttaatttctactagagtcacacaggaaag
BBa_B0032_sequence
1
tcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z