BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_K101011
1
BBa_K101011
Dual-Repressed Promoter for LacI and Lambda cI + RBS (med strength)
2008-07-27T11:00:00Z
2015-05-08T01:08:42Z
This part combines BBa_K101001, the dually-repressed promoter, with BBa_B0032, a medium strength RBS with good characterization and experience by other registry users.
This promoter will be repressed in the presence of the LacI protein from E. coli, the cI protein from the Lambda phage, or both. A medium strength RBS has been incorporated for convenient cloning to a desired gene.
false
false
_205_
0
3296
9
Not in stock
false
This promoter contains two lambda cI operator sites; one upstream of the -35 polymerase binding site, and another between the -35 and -10 binding sites. It also includes a LacI operator site downstream of the -10 polymerase binding site. -35 and -10 sites were derived from the cI repressed promoter from lambda phage. A CAP binding site is also included for effective repression. For information concerning the RBS design, please see part BBa_B0032.
false
Kristen J Lindblad
component1968915
1
BBa_B0032
component1968913
1
BBa_K101001
annotation1968915
1
BBa_B0032
range1968915
1
125
137
annotation1968913
1
BBa_K101001
range1968913
1
1
116
BBa_K101001
1
BBa_K101001
Dual-Repressed Promoter for LacI and LambdacI
2008-07-27T11:00:00Z
2015-05-08T01:08:41Z
This part was made by combining BioBrick parts R0010(LacI promoter) and R0051 (LambdacI promoter), along with intermediate sequence between these operator sites.
This part is a new construct of a promoter that is dually repressed by either LacI or LambdacI proteins.
false
false
_205_
0
3363
9
Not in stock
false
LambdacI usually binds to two operator sites, one located upstream from -35 and one located between -35 and -10. By itself, LacI binds downstream from -10. This made the part design very straightforward, since the locations of the operator sites do not overlap at all. In order to complete this part, we simply combined the proper sequences and we had a dually-repressed promoter.
false
Bennett Swiniarski
BBa_K101011_sequence
1
gcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggcggtgataatgtgtggaattgtgagcggataaaatttcacacatactagagtcacacaggaaag
BBa_K101001_sequence
1
gcgcaacgcaattaatgtgagttagctcactcattaggcataacaccgtgcgtgttgactattttacctctggcggtgataatgtgtggaattgtgagcggataaaatttcacaca
BBa_B0032_sequence
1
tcacacaggaaag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z