LOCUS       BBa_K101016              945 bp    DNA     linear       29-May-2024
DEFINITION  Dually repressed promoter with sites for TetR and P22MNT binding
ACCESSION   BBa_K101016
VERSION     BBa_K101016.1  
FEATURES             Location/Qualifiers
     promoter        1..61
                     /label=BBa_K101000
     RBS             70..82
                     /label=RBS-3\Weak
     RBS             76..79
                     /label=RBS
     misc_feature    70..82
                     /label=BBa_B0032
     RBS             70..82
                     /label=BBa_B0032
     CDS             89..808
                     /label=GFP protein
     CDS             89..808
                     /label=BBa_E0040
     stem_loop       828..871
                     /label=stem_loop
     misc_feature    817..896
                     /label=BBa_B0010
     terminator      817..896
                     /label=BBa_B0010
     stem_loop       912..931
                     /label=T7 TE
     misc_feature    938..938
                     /label=stop
     polyA_site      932..945
                     /label=polya
     misc_feature    905..945
                     /label=BBa_B0012
     terminator      905..945
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacaaggtc cacggtgacc tagatctccg atactgagca
       61 ctactagagt cacacaggaa agtactagat gcgtaaagga gaagaacttt tcactggagt
      121 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
      181 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
      241 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
      301 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
      361 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
      421 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
      481 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
      541 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
      601 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
      661 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
      721 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
      781 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg
      841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//