BBa_I15040 1 RBS.TetR RBS.TetR from pASKIBA3 2005-06-01T11:00:00Z 2015-08-31T04:07:39Z pASKIBA3 (IBA biotagnology) The part accepts POPS to produce TetR. This sequence encodes the ribosome binding site and tetracycline repressor protein from the plasmid pASKIBA3. false false _5_ 0 88 37 It's complicated true false Jeff Tabor annotation1501146 1 paskiba3_TetR range1501146 1 16 642 annotation1501145 1 paskiba3_rbs range1501145 1 1 15 BBa_K101017 1 MioC MioC Promoter (DNAa-Repressed Promoter) 2008-10-27T12:00:00Z 2015-05-08T01:08:42Z The source of this part is from the genomic dna of E.coli (JM109). The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109). This promoter can be used in series with a reporter to signal only during a given part of the cell cycle. false false _205_ 0 2728 9 It's complicated false Site directed mutagenesis was used to create a Spe1 restriction site. false Karl Petersen annotation1992601 1 DNAa Binding Box range1992601 1 295 303 annotation1992615 1 MioC Promoter range1992615 1 131 319 annotation1992609 1 DNAa Binding Box range1992609 1 159 167 annotation1992607 1 DNAa Binding Box range1992607 1 273 279 annotation1992605 1 DNAa Binding Box range1992605 1 283 292 BBa_K101018 1 BBa_K101018 MioC Promoter (regulating tetR) 2008-10-28T12:00:00Z 2015-05-08T01:08:42Z The MioC promoter is from K-12 and the tetR is the part BBa_I15040. The MioC promoter is a cell-cycle dependent promoter that is repressed before initiation of replication and depressed shortly after. The regulation of the MioC gene is overseen by several DNAa proteins, which bind along the promoter. This particular MioC promoter was isolated from K-12 (JM109). This promoter is upstream of a tetR (BBa_I15040), which is repressed by the binding of DNAa during initiation. false false _205_ 0 2728 9 It's complicated false Site directed mutagenesis was used to create a Spe1 restriction site. false Karl Petersen component1995342 1 BBa_I15040 component1995339 1 BBa_K101017 annotation1995339 1 BBa_K101017 range1995339 1 1 319 annotation1995342 1 BBa_I15040 range1995342 1 328 969 BBa_K101018_sequence 1 gaattcgcggccgcttctagaggagcgccaaagactacccttccgcgctggcaaagctggaaagccttgatgaagtcactgaagcctattacacaaccggccactacagcatctttataaaagtgatgtgccgttcgatcgacgctctccagcatgtacttatcaacaagatccaaacaattgatgaaattcagtccaccgagacattgatcgtcctgcaaaacccgatcatgcgtaccatcaagccctgatcggcttttttaatcccatacttttccacaggtagatcccaacgcgttcacagcgtacaattactagttactagagcattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaa BBa_K101017_sequence 1 gaattcgcggccgcttctagaggagcgccaaagactacccttccgcgctggcaaagctggaaagccttgatgaagtcactgaagcctattacacaaccggccactacagcatctttataaaagtgatgtgccgttcgatcgacgctctccagcatgtacttatcaacaagatccaaacaattgatgaaattcagtccaccgagacattgatcgtcctgcaaaacccgatcatgcgtaccatcaagccctgatcggcttttttaatcccatacttttccacaggtagatcccaacgcgttcacagcgtacaattactagt BBa_I15040_sequence 1 cattggtaggaattaatgatgtctcgtttagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgcagtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z