LOCUS       BBa_K1011001            2116 bp    DNA     linear       27-Dec-2024
DEFINITION  P + RBS + ftsZ + RBS + GFP + Term
ACCESSION   BBa_K1011001
VERSION     BBa_K1011001.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    1..75
                     /label=BBa_K215000
     CDS             82..1233
                     /label=BBa_K1011000
     misc_feature    1..1233
                     /label=BBa_S05213
     misc_feature    1246..1249
                     /label=conserved
     RBS             1242..1253
                     /label=BBa_B0034
     CDS             1260..1979
                     /label=GFP protein
     CDS             1260..1979
                     /label=BBa_E0040
     misc_feature    1242..1979
                     /label=BBa_S05211
     stem_loop       1999..2042
                     /label=stem_loop
     misc_feature    1988..2067
                     /label=BBa_B0010
     terminator      1988..2067
                     /label=BBa_B0010
     stem_loop       2083..2102
                     /label=T7 TE
     misc_feature    2109..2109
                     /label=stop
     polyA_site      2103..2116
                     /label=polya
     misc_feature    2076..2116
                     /label=BBa_B0012
     terminator      2076..2116
                     /label=BBa_B0012
     terminator      1988..2116
                     /label=BBa_B0015
     misc_feature    1242..2116
                     /label=BBa_S05212
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtttgaa ccaatggaac ttaccaatga cgcggtgatt
      121 aaagtcatcg gcgtcggcgg cggcggcggt aatgctgttg aacacatggt gcgcgagcgc
      181 attgaaggtg ttgaattttt cgcggtaaat accgatgcac aagcgctgcg taaaacagcg
      241 gttggacaga cgattcaaat cggtagcggt atcaccaaag gactgggcgc tggcgctaat
      301 ccagaagttg gccgcaatgc ggctgatgag gatcgcgatg cattgcgtgc ggcgctggaa
      361 ggtgcagaca tggtctttat tgctgcgggt atgggtggtg gtaccggtac aggtgcagca
      421 ccagtcgtcg ctgaagtggc aaaagatttg ggtatcctga ccgttgctgt cgtcactaag
      481 cctttcaact ttgaaggcaa gaagcgtatg gcattcgcgg agcaggggat cactgaactg
      541 tccaagcatg tggactctct gatcactatc ccgaacgaca aactgctgaa agttctgggc
      601 cgcggtatct ccctgctgga tgcgtttggc gcagcgaacg atgtactgaa aggcgctgtg
      661 caaggtatcg ctgaactgat tactcgtccg ggtttgatga acgtggactt tgcagacgta
      721 cgcaccgtaa tgtctgagat gggctacgca atgatgggtt ctggcgtggc gagcggtgaa
      781 gaccgtgcgg aagaagctgc tgaaatggct atctcttctc cgctgctgga agatatcgac
      841 ctgtctggcg cgcgcggcgt gctggttaac atcacggcgg gcttcgacct gcgtctggat
      901 gagttcgaaa cggtaggtaa caccatccgt gcatttgctt ccgacaacgc gactgtggtt
      961 atcggtactt ctcttgaccc ggatatgaat gacgagctgc gcgtaaccgt tgttgcgaca
     1021 ggtatcggca tggacaaacg tcctgaaatc actctggtga ccaataagca ggttcagcag
     1081 ccagtgatgg atcgctacca gcagcatggg atggctccgc tgacccagga gcagaagccg
     1141 gttgctaaag tcgtgaatga caatgcgccg caaactgcga aagagccgga ttatctggat
     1201 atcccagcat tcctgcgtaa gcaagctgat taatactaga gaaagaggag aaatactaga
     1261 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1321 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1381 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1441 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1501 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1561 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1621 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1681 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1741 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1801 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1861 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1921 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1981 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2041 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2101 cctttctgcg tttata
//