BBa_K1011001
type: ComponentDefinition
persistent identity: BBa K1011001
display id: BBa_K1011001
version: 1
was generated by: iGEM to SBOL conversion
description: P + RBS + ftsZ + RBS + GFP + Term
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: The biobricks are all previous submissions. The novel part of this biobrick is the ftsZ gene, biobrick K1011000.
owned by: james
owned by: myers
mutable description: This biobrick enables the complete production of ftsZ and GFP (as an indicator of production). The same RBS is used upstream of both ftsZ and GFP in order to approximate a 1:1 production, so that the flourescent intensity will give a relative indication of ftsZ production.
discontinued: false
dominant: false
experience: experience/None
group u list: _1317_
m user id: 0
owner id: 14270
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: VGEM 2013
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S05212
component: BBa_S05213
sequence annotation: BBa_S05213
sequence annotation: BBa_S05212
sequence: https://synbiohub.org/public/igem/BBa_K1011001_sequence/1

BBa_S05212
type: Component
persistent identity: component2339250
display id: component2339250
version: 1
top level: BBa_K1011001
definition: BBa_S05212
access: public

BBa_S05213
type: Component
persistent identity: component2339237
display id: component2339237
version: 1
top level: BBa_K1011001
definition: BBa_S05213
access: public

BBa_S05213
type: SequenceAnnotation
persistent identity: annotation2339237
display id: annotation2339237
version: 1
top level: BBa_K1011001
location: https://synbiohub.org/public/igem/BBa_K1011001/annotation2339237/range2339237/1
component: BBa_S05213

https://synbiohub.org/public/igem/BBa_K1011001/annotation2339237/range2339237/1
type: Range
persistent identity: range2339237
display id: range2339237
version: 1
top level: BBa_K1011001
direction: direction/reverse
start: 1
end: 1233
orientation: inline

BBa_S05212
type: SequenceAnnotation
persistent identity: annotation2339250
display id: annotation2339250
version: 1
top level: BBa_K1011001
location: https://synbiohub.org/public/igem/BBa_K1011001/annotation2339250/range2339250/1
component: BBa_S05212

https://synbiohub.org/public/igem/BBa_K1011001/annotation2339250/range2339250/1
type: Range
persistent identity: range2339250
display id: range2339250
version: 1
top level: BBa_K1011001
direction: direction/reverse
start: 1242
end: 2116
orientation: inline

BBa_S05212
type: ComponentDefinition
persistent identity: BBa S05212
display id: BBa_S05212
version: 1
was generated by: iGEM to SBOL conversion
description: S05211:B0015
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 14270
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Shaun Moshasha
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0015
component: BBa_S05211
sequence annotation: BBa_S05211
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_S05212_sequence/1

BBa_B0015
type: Component
persistent identity: component2339202
display id: component2339202
version: 1
top level: BBa_S05212
definition: BBa_B0015
access: public

BBa_S05211
type: Component
persistent identity: component2339195
display id: component2339195
version: 1
top level: BBa_S05212
definition: BBa_S05211
access: public

BBa_S05211
type: SequenceAnnotation
persistent identity: annotation2339195
display id: annotation2339195
version: 1
top level: BBa_S05212
location: https://synbiohub.org/public/igem/BBa_S05212/annotation2339195/range2339195/1
component: BBa_S05211

https://synbiohub.org/public/igem/BBa_S05212/annotation2339195/range2339195/1
type: Range
persistent identity: range2339195
display id: range2339195
version: 1
top level: BBa_S05212
direction: direction/reverse
start: 1
end: 738
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2339202
display id: annotation2339202
version: 1
top level: BBa_S05212
location: https://synbiohub.org/public/igem/BBa_S05212/annotation2339202/range2339202/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_S05212/annotation2339202/range2339202/1
type: Range
persistent identity: range2339202
display id: range2339202
version: 1
top level: BBa_S05212
direction: direction/reverse
start: 747
end: 875
orientation: inline

BBa_S05213
type: ComponentDefinition
persistent identity: BBa S05213
display id: BBa_S05213
version: 1
was generated by: iGEM to SBOL conversion
description: K215000:K1011000
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 14270
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Shaun Moshasha
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K1011000
component: BBa_K215000
sequence annotation: BBa_K215000
sequence annotation: BBa_K1011000
sequence: https://synbiohub.org/public/igem/BBa_S05213_sequence/1

BBa_K1011000
type: Component
persistent identity: component2339211
display id: component2339211
version: 1
top level: BBa_S05213
definition: FtsZ
access: public

BBa_K215000
type: Component
persistent identity: component2339210
display id: component2339210
version: 1
top level: BBa_S05213
definition: BBa_K215000
access: public

BBa_K215000
type: SequenceAnnotation
persistent identity: annotation2339210
display id: annotation2339210
version: 1
top level: BBa_S05213
location: https://synbiohub.org/public/igem/BBa_S05213/annotation2339210/range2339210/1
component: BBa_K215000

https://synbiohub.org/public/igem/BBa_S05213/annotation2339210/range2339210/1
type: Range
persistent identity: range2339210
display id: range2339210
version: 1
top level: BBa_S05213
direction: direction/reverse
start: 1
end: 75
orientation: inline

BBa_K1011000
type: SequenceAnnotation
persistent identity: annotation2339211
display id: annotation2339211
version: 1
top level: BBa_S05213
location: https://synbiohub.org/public/igem/BBa_S05213/annotation2339211/range2339211/1
component: BBa_K1011000

https://synbiohub.org/public/igem/BBa_S05213/annotation2339211/range2339211/1
type: Range
persistent identity: range2339211
display id: range2339211
version: 1
top level: BBa_S05213
direction: direction/reverse
start: 82
end: 1233
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K215000
type: ComponentDefinition
persistent identity: BBa K215000
display id: BBa_K215000
version: 1
was generated by: iGEM to SBOL conversion
description: R0011+B0034, strong IPTG-inducible promoter with strong RBS.
created: 2009-09-29T11:00:00Z
modified: 2015-05-08T01:11:29Z
mutable provenance: existing registry parts
owned by: james
owned by: myers
mutable description: This part contains the strong, IPTG-inducible promoter R0011, combined with the strong RBS B0034. This part can be used as a strong protein expression system when combined with a protein coding sequence.
discontinued: false
dominant: false
experience: experience/Works
group u list: _320_
m user id: 0
owner id: 2811
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: This part should offer greater levels of expression when compared to similar parts using the natural lac promoter (R0010).
star: false
creator: Chris Eiben
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_K215000_sequence/1

BBa_B0034
type: Component
persistent identity: component2027441
display id: component2027441
version: 1
top level: BBa_K215000
definition: BBa_B0034
access: public

BBa_R0011
type: Component
persistent identity: component2027435
display id: component2027435
version: 1
top level: BBa_K215000
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2027435
display id: annotation2027435
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K215000/annotation2027435/range2027435/1
type: Range
persistent identity: range2027435
display id: range2027435
version: 1
top level: BBa_K215000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2027441
display id: annotation2027441
version: 1
top level: BBa_K215000
location: https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K215000/annotation2027441/range2027441/1
type: Range
persistent identity: range2027441
display id: range2027441
version: 1
top level: BBa_K215000
direction: direction/reverse
start: 64
end: 75
orientation: inline

FtsZ
type: ComponentDefinition
persistent identity: BBa K1011000
display id: BBa_K1011000
version: 1
was generated by: iGEM to SBOL conversion
description: ftsZ gene from E. coli on pSB1C3
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: The organism for which we based our sequence is Escherichia coli str. K-12 substr. MG1655, located at the following NCBI url: http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.2?report=genbank&from=105305&to=106456
owned by: james
owned by: myers
mutable description: This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli mini-cells.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1317_
m user id: 0
owner id: 14270
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: There is an illegal EcoR1 restriction site at bp 102-107 of the gene. We changed the C at site 107 to a T, which maintained the codon optimization and removed the illegal restriction site.
star: false
creator: VGEM 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K1011000_sequence/1

BBa_S05211
type: ComponentDefinition
persistent identity: BBa S05211
display id: BBa_S05211
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:E0040
created: 2013-09-11T11:00:00Z
modified: 2015-05-08T01:14:51Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 14270
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Shaun Moshasha
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_E0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_S05211_sequence/1

BBa_E0040
type: Component
persistent identity: component2339190
display id: component2339190
version: 1
top level: BBa_S05211
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2339188
display id: component2339188
version: 1
top level: BBa_S05211
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2339188
display id: annotation2339188
version: 1
top level: BBa_S05211
location: https://synbiohub.org/public/igem/BBa_S05211/annotation2339188/range2339188/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S05211/annotation2339188/range2339188/1
type: Range
persistent identity: range2339188
display id: range2339188
version: 1
top level: BBa_S05211
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2339190
display id: annotation2339190
version: 1
top level: BBa_S05211
location: https://synbiohub.org/public/igem/BBa_S05211/annotation2339190/range2339190/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_S05211/annotation2339190/range2339190/1
type: Range
persistent identity: range2339190
display id: range2339190
version: 1
top level: BBa_S05211
direction: direction/reverse
start: 19
end: 738
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K1011001_sequence/1
type: Sequence
persistent identity: BBa K1011001 sequence
display id: BBa_K1011001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtttgaaccaatggaacttaccaatgacgcggtgattaaagtcatcggcgtcggcggcggcggcggtaatgctgttgaacacatggtgcgcgagcgcattgaaggtgttgaatttttcgcggtaaataccgatgcacaagcgctgcgtaaaacagcggttggacagacgattcaaatcggtagcggtatcaccaaaggactgggcgctggcgctaatccagaagttggccgcaatgcggctgatgaggatcgcgatgcattgcgtgcggcgctggaaggtgcagacatggtctttattgctgcgggtatgggtggtggtaccggtacaggtgcagcaccagtcgtcgctgaagtggcaaaagatttgggtatcctgaccgttgctgtcgtcactaagcctttcaactttgaaggcaagaagcgtatggcattcgcggagcaggggatcactgaactgtccaagcatgtggactctctgatcactatcccgaacgacaaactgctgaaagttctgggccgcggtatctccctgctggatgcgtttggcgcagcgaacgatgtactgaaaggcgctgtgcaaggtatcgctgaactgattactcgtccgggtttgatgaacgtggactttgcagacgtacgcaccgtaatgtctgagatgggctacgcaatgatgggttctggcgtggcgagcggtgaagaccgtgcggaagaagctgctgaaatggctatctcttctccgctgctggaagatatcgacctgtctggcgcgcgcggcgtgctggttaacatcacggcgggcttcgacctgcgtctggatgagttcgaaacggtaggtaacaccatccgtgcatttgcttccgacaacgcgactgtggttatcggtacttctcttgacccggatatgaatgacgagctgcgcgtaaccgttgttgcgacaggtatcggcatggacaaacgtcctgaaatcactctggtgaccaataagcaggttcagcagccagtgatggatcgctaccagcagcatgggatggctccgctgacccaggagcagaagccggttgctaaagtcgtgaatgacaatgcgccgcaaactgcgaaagagccggattatctggatatcccagcattcctgcgtaagcaagctgattaatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05212_sequence/1
type: Sequence
persistent identity: BBa S05212 sequence
display id: BBa_S05212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05213_sequence/1
type: Sequence
persistent identity: BBa S05213 sequence
display id: BBa_S05213_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgtttgaaccaatggaacttaccaatgacgcggtgattaaagtcatcggcgtcggcggcggcggcggtaatgctgttgaacacatggtgcgcgagcgcattgaaggtgttgaatttttcgcggtaaataccgatgcacaagcgctgcgtaaaacagcggttggacagacgattcaaatcggtagcggtatcaccaaaggactgggcgctggcgctaatccagaagttggccgcaatgcggctgatgaggatcgcgatgcattgcgtgcggcgctggaaggtgcagacatggtctttattgctgcgggtatgggtggtggtaccggtacaggtgcagcaccagtcgtcgctgaagtggcaaaagatttgggtatcctgaccgttgctgtcgtcactaagcctttcaactttgaaggcaagaagcgtatggcattcgcggagcaggggatcactgaactgtccaagcatgtggactctctgatcactatcccgaacgacaaactgctgaaagttctgggccgcggtatctccctgctggatgcgtttggcgcagcgaacgatgtactgaaaggcgctgtgcaaggtatcgctgaactgattactcgtccgggtttgatgaacgtggactttgcagacgtacgcaccgtaatgtctgagatgggctacgcaatgatgggttctggcgtggcgagcggtgaagaccgtgcggaagaagctgctgaaatggctatctcttctccgctgctggaagatatcgacctgtctggcgcgcgcggcgtgctggttaacatcacggcgggcttcgacctgcgtctggatgagttcgaaacggtaggtaacaccatccgtgcatttgcttccgacaacgcgactgtggttatcggtacttctcttgacccggatatgaatgacgagctgcgcgtaaccgttgttgcgacaggtatcggcatggacaaacgtcctgaaatcactctggtgaccaataagcaggttcagcagccagtgatggatcgctaccagcagcatgggatggctccgctgacccaggagcagaagccggttgctaaagtcgtgaatgacaatgcgccgcaaactgcgaaagagccggattatctggatatcccagcattcctgcgtaagcaagctgattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K215000_sequence/1
type: Sequence
persistent identity: BBa K215000 sequence
display id: BBa_K215000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1011000_sequence/1
type: Sequence
persistent identity: BBa K1011000 sequence
display id: BBa_K1011000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtttgaaccaatggaacttaccaatgacgcggtgattaaagtcatcggcgtcggcggcggcggcggtaatgctgttgaacacatggtgcgcgagcgcattgaaggtgttgaatttttcgcggtaaataccgatgcacaagcgctgcgtaaaacagcggttggacagacgattcaaatcggtagcggtatcaccaaaggactgggcgctggcgctaatccagaagttggccgcaatgcggctgatgaggatcgcgatgcattgcgtgcggcgctggaaggtgcagacatggtctttattgctgcgggtatgggtggtggtaccggtacaggtgcagcaccagtcgtcgctgaagtggcaaaagatttgggtatcctgaccgttgctgtcgtcactaagcctttcaactttgaaggcaagaagcgtatggcattcgcggagcaggggatcactgaactgtccaagcatgtggactctctgatcactatcccgaacgacaaactgctgaaagttctgggccgcggtatctccctgctggatgcgtttggcgcagcgaacgatgtactgaaaggcgctgtgcaaggtatcgctgaactgattactcgtccgggtttgatgaacgtggactttgcagacgtacgcaccgtaatgtctgagatgggctacgcaatgatgggttctggcgtggcgagcggtgaagaccgtgcggaagaagctgctgaaatggctatctcttctccgctgctggaagatatcgacctgtctggcgcgcgcggcgtgctggttaacatcacggcgggcttcgacctgcgtctggatgagttcgaaacggtaggtaacaccatccgtgcatttgcttccgacaacgcgactgtggttatcggtacttctcttgacccggatatgaatgacgagctgcgcgtaaccgttgttgcgacaggtatcggcatggacaaacgtcctgaaatcactctggtgaccaataagcaggttcagcagccagtgatggatcgctaccagcagcatgggatggctccgctgacccaggagcagaagccggttgctaaagtcgtgaatgacaatgcgccgcaaactgcgaaagagccggattatctggatatcccagcattcctgcgtaagcaagctgattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S05211_sequence/1
type: Sequence
persistent identity: BBa S05211 sequence
display id: BBa_S05211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z