BBa_K1012002
1
BBa_K1012002
The TorA twin-arginine signal sequence
2013-08-28T11:00:00Z
2015-05-08T01:08:42Z
Published paper, reference needed
Protein phosphatase 1 (PP1,human protein) with N terminal TorA signal sequence and C terminal hemagglutinin (HA) tag.
TorA signal sequence is taken from E. coli TMAO reductase. This will target the protein for Tat transport.
HA tag is attached for identification of PP1 with Western blots.
false
false
_1318_
0
15739
9
It's complicated
true
PP1 contained PstI site, this was removed by quick change.
TorA signal sequence was added by standard cloning proceudres.
false
John Allan
BBa_K1012002_sequence
1
atgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtggccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z