BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1014001
1
BBa_K1014001
HrpR Gene
2013-08-26T11:00:00Z
2015-05-08T01:08:42Z
E.coli
This codes for HrpR protein which is needed for hrpL AND Gate
false
false
_999_1320_
0
11931
9
In stock
false
ATGTCTACCGACATCGACAACGACGTTCTGACCTGCTGCAACGTTACCGCTCTGTCTGCTGGTCACCAGATCGTTATGAACTCTGTTCTGATGGACATGGACCTGCTGCTGTGCGGTGAAACCGGTACCGGTAAAGACACCCTGGCTTCTCGTATCCACGAACTGTCTTCTCGTACCGGTCCGTTCGTTGGTATGAACTGCGCTGCTATCCCGGAATCTCTGGCTGAATCTCAGCTGTTCGGTGTTGTTAACGGTGCTTTTACTGGTGTCTGCCGTGCTCGTGAAGGTTACATCGAAGCTTCTTCTGGTGGTACCCTGTACCTGGACGAAATCGACTCTATGCCGCTGTCTCTGCAAGCTAAACTGCTGCGTGTTCTGGAATCTCGTGGTGTTGAACGTCTGGGTTCTACCGACTTCATCCCGCTGGACCTGCGTGTTATCGCTTCTGCTCAGCGTCCGCTGGACGAACTGGTTGAACAGGGTCTGTTCCGTCGTGACCTGTTCTTCCGTCTGAACGTTCTGACCCTGCAACTGCCGGCTCTGCGTAAACGTCGTGAACAGATCCTGCCGCTGTTCGACCAGTTCACCCAGGACGTTGCTGCTGAATCTGGTCGTTCTGTTCCGACCCTGGACAACCGTCGTGTTCAGATCCTGCTGTCTCACGACTGGCCGGGTAACGTTCGTGAACTGAAATCTGCTGCTAAACGTTTCGTTCTGGGTCTGCCGCTGCTGGGTGCTGAACCGGTTGAAGCTCGTGACCCGGTTACCGGTCTGCGTATGCAGATGCGTGTTATCGAAAAAATGCTGATCCAGGACGCTCTGAAACGTCACCGTCACAACTTCGACGCTGTTCTGGAAGAACTGGAACTGCCGCGTCGTACCCTGTACCACCGTATGAAAGAACTGGGTGTTGCTTCTCACATCGACCTGGTTGCTGAATCTTAATAA
false
Xiangmiao Zeng
BBa_K1014003
1
BBa_K1014003
HrpR Protein Generator(S)
2013-08-28T11:00:00Z
2015-05-08T01:08:42Z
E.coli
This device will produce HrpR protein treated with IPTG under strong RBS.
false
false
_999_1320_
0
11931
9
It's complicated
false
JCAT
false
Xiangmiao Zeng
component2333378
1
BBa_B0012
component2333375
1
BBa_K1014001
component2333373
1
BBa_B0030
component2333376
1
BBa_B0010
component2333367
1
BBa_R0011
annotation2333376
1
BBa_B0010
range2333376
1
1041
1120
annotation2333373
1
BBa_B0030
range2333373
1
64
78
annotation2333367
1
BBa_R0011
range2333367
1
1
54
annotation2333375
1
BBa_K1014001
range2333375
1
85
1032
annotation2333378
1
BBa_B0012
range2333378
1
1129
1169
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
annotation1999
1
lac O1
range1999
1
3
19
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1014001_sequence
1
atgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1014003_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z