BBa_K1014001
1
BBa_K1014001
HrpR Gene
2013-08-26T11:00:00Z
2015-05-08T01:08:42Z
E.coli
This codes for HrpR protein which is needed for hrpL AND Gate
false
false
_999_1320_
0
11931
9
In stock
false
ATGTCTACCGACATCGACAACGACGTTCTGACCTGCTGCAACGTTACCGCTCTGTCTGCTGGTCACCAGATCGTTATGAACTCTGTTCTGATGGACATGGACCTGCTGCTGTGCGGTGAAACCGGTACCGGTAAAGACACCCTGGCTTCTCGTATCCACGAACTGTCTTCTCGTACCGGTCCGTTCGTTGGTATGAACTGCGCTGCTATCCCGGAATCTCTGGCTGAATCTCAGCTGTTCGGTGTTGTTAACGGTGCTTTTACTGGTGTCTGCCGTGCTCGTGAAGGTTACATCGAAGCTTCTTCTGGTGGTACCCTGTACCTGGACGAAATCGACTCTATGCCGCTGTCTCTGCAAGCTAAACTGCTGCGTGTTCTGGAATCTCGTGGTGTTGAACGTCTGGGTTCTACCGACTTCATCCCGCTGGACCTGCGTGTTATCGCTTCTGCTCAGCGTCCGCTGGACGAACTGGTTGAACAGGGTCTGTTCCGTCGTGACCTGTTCTTCCGTCTGAACGTTCTGACCCTGCAACTGCCGGCTCTGCGTAAACGTCGTGAACAGATCCTGCCGCTGTTCGACCAGTTCACCCAGGACGTTGCTGCTGAATCTGGTCGTTCTGTTCCGACCCTGGACAACCGTCGTGTTCAGATCCTGCTGTCTCACGACTGGCCGGGTAACGTTCGTGAACTGAAATCTGCTGCTAAACGTTTCGTTCTGGGTCTGCCGCTGCTGGGTGCTGAACCGGTTGAAGCTCGTGACCCGGTTACCGGTCTGCGTATGCAGATGCGTGTTATCGAAAAAATGCTGATCCAGGACGCTCTGAAACGTCACCGTCACAACTTCGACGCTGTTCTGGAAGAACTGGAACTGCCGCGTCGTACCCTGTACCACCGTATGAAAGAACTGGGTGTTGCTTCTCACATCGACCTGGTTGCTGAATCTTAATAA
false
Xiangmiao Zeng
BBa_K1014004
1
BBa_K1014004
HrpR Protein Generator(W)
2013-08-28T11:00:00Z
2015-05-08T01:08:42Z
E.coli
This device will produce HrpR protein treated with IPTG under weak RBS.
false
false
_999_1320_
0
11931
9
In stock
false
JCAT
false
Xiangmiao Zeng
component2334772
1
BBa_B0010
component2334774
1
BBa_B0012
component2334771
1
BBa_K1014001
component2334764
1
BBa_R0011
component2334770
1
BBa_B0031
annotation2334772
1
BBa_B0010
range2334772
1
1040
1119
annotation2334774
1
BBa_B0012
range2334774
1
1128
1168
annotation2334770
1
BBa_B0031
range2334770
1
64
77
annotation2334764
1
BBa_R0011
range2334764
1
1
54
annotation2334771
1
BBa_K1014001
range2334771
1
84
1031
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
BBa_B0031
1
BBa_B0031
RBS.2 (weak) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23316
1
conserved
range23316
1
7
10
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2001
1
lac O1
range2001
1
26
42
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
annotation2000
1
-35
range2000
1
20
25
annotation2002
1
-10
range2002
1
43
48
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1014001_sequence
1
atgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataa
BBa_B0031_sequence
1
tcacacaggaaacc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
BBa_K1014004_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaacctactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z