LOCUS BBa_K1014005 1207 bp DNA linear 22-Nov-2024 DEFINITION HrpS Protein Generator(S) ACCESSION BBa_K1014005 VERSION BBa_K1014005.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..1070 /label=BBa_K1014000 stem_loop 1090..1133 /label=stem_loop misc_feature 1079..1158 /label=BBa_B0010 terminator 1079..1158 /label=BBa_B0010 stem_loop 1174..1193 /label=T7 TE misc_feature 1200..1200 /label=stop polyA_site 1194..1207 /label=polya misc_feature 1167..1207 /label=BBa_B0012 terminator 1167..1207 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgacca tccgtaactc taacgaatct gttcagtctc 121 tgcactgcgc ttctcacgaa tcttctctga cccacccgtc tgaagacatc cacggttctc 181 tgtcttctct gatccacacc atcgctccgc tgaacgttga catcgttctg gaaggtgaaa 241 ccggtaccgg taaagacacc ctggctcgtc gtatccacca gctgtctggt tgcgctggtc 301 cgctggttgc tgttaactgc gctgctgttc cggaaaccct ggctgaatct gaactgttcg 361 gtgttgtttc tggtgcttac accggtgctt ctcgttctcg tgctggttac ctggaaaccg 421 ctgacaaagg tatcctgttc ctggacgaaa tcgactctat gccgctgacc ctgcaagcta 481 aaatgctgcg tgttctggaa tctcgtggta tcgaacgtct gggttctacc cagttcaaac 541 cggttgacat gcgtgttatc gttgctaccc agaccccgct gcaaaaactg gttgacgaag 601 gtcgtttccg tcgtgacctg tacttccgtc tggacaccgt taaaatccag ctgccgaccc 661 tgcgttctcg taccgaaatc atcctgccgc tgttcgaacg tttcctgctg gaagcttctg 721 ctcgtctgaa acgtccggct ccgggtctgt ctgctatcat ccaggaacag ctgctgatgc 781 acggttggcc gggtaacatc cgtgaactga aagctgctgc tgaacgttgg gttctgggtc 841 tgtctccggt tccgatcgct tctgacggtg acgttcaggt tatgggtgaa gacaccccgc 901 tgacctctct gaaagttcgt ctgcgtcgta tagagaggtt cctgatccag gaggctctgc 961 aacgtaacga ccactgcatc gacaccgttg ttaacgaact gggtatcccg aaacgtaccc 1021 tgtaccaccg tatcaaactg ctgaacgttg ctgctcgtgg tctgtaataa tactagagcc 1081 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1141 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1201 gtttata //