LOCUS       BBa_K1014008            1061 bp    DNA     linear       22-Dec-2024
DEFINITION  tetR Protein Generator(S)
ACCESSION   BBa_K1014008
VERSION     BBa_K1014008.1  
FEATURES             Location/Qualifiers
     promoter        1..210
                     /label=BBa_K1014002
     RBS             219..233
                     /label=RBS-1\Strong
     RBS             226..230
                     /label=RBS
     misc_feature    219..233
                     /label=BBa_B0030
     RBS             219..233
                     /label=BBa_B0030
     CDS             243..859
                     /label=tetR
     misc_feature    860..893
                     /label=SsrA
     misc_feature    900..924
                     /label=Help:Barcodes
     CDS             240..924
                     /label=BBa_C0040
     stem_loop       944..987
                     /label=stem_loop
     misc_feature    933..1012
                     /label=BBa_B0010
     terminator      933..1012
                     /label=BBa_B0010
     stem_loop       1028..1047
                     /label=T7 TE
     misc_feature    1054..1054
                     /label=stop
     polyA_site      1048..1061
                     /label=polya
     misc_feature    1021..1061
                     /label=BBa_B0012
     terminator      1021..1061
                     /label=BBa_B0012
ORIGIN
        1 gccggattat gtccgctgag tgggtcacgg tcccggatca gttcccttgc gaagctgacc
       61 gatgtttttg atgccaaaag ctgttgtggc aaaaaacggt ttgcgcaaag ttttgtatta
      121 caaagaattt cacattttaa atatatcttt ataaatcaat cagttatttc tatttttaag
      181 ctggcatggt tatcgctata gggcttgtac tactagagat taaagaggag aaatactaga
      241 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      301 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      361 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      421 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      481 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
      541 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
      601 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
      661 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
      721 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
      781 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
      841 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
      901 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
      961 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1021 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//