LOCUS BBa_K1014014 3474 bp DNA linear 15-Nov-2024 DEFINITION hrpL AND Gate device ACCESSION BBa_K1014014 VERSION BBa_K1014014.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 CDS 85..1032 /label=BBa_K1014001 stem_loop 1052..1095 /label=stem_loop misc_feature 1041..1120 /label=BBa_B0010 terminator 1041..1120 /label=BBa_B0010 stem_loop 1136..1155 /label=T7 TE misc_feature 1162..1162 /label=stop polyA_site 1156..1169 /label=polya misc_feature 1129..1169 /label=BBa_B0012 terminator 1129..1169 /label=BBa_B0012 misc_feature 1..1169 /label=BBa_K1014003 misc_feature 1178..1196 /label=TetR 1 misc_feature 1178..1231 /label=BBa_R0040 promoter 1197..1202 /label=-35 misc_feature 1203..1221 /label=TetR 2 promoter 1220..1225 /label=-10 promoter 1178..1231 /label=BBa_R0040 RBS 1240..1254 /label=RBS-1\Strong RBS 1247..1251 /label=RBS misc_feature 1240..1254 /label=BBa_B0030 RBS 1240..1254 /label=BBa_B0030 CDS 1261..2247 /label=BBa_K1014000 stem_loop 2267..2310 /label=stem_loop misc_feature 2256..2335 /label=BBa_B0010 terminator 2256..2335 /label=BBa_B0010 stem_loop 2351..2370 /label=T7 TE misc_feature 2377..2377 /label=stop polyA_site 2371..2384 /label=polya misc_feature 2344..2384 /label=BBa_B0012 terminator 2344..2384 /label=BBa_B0012 misc_feature 1178..2384 /label=BBa_K1014005 promoter 2393..2602 /label=BBa_K1014002 RBS 2611..2625 /label=RBS-1\Strong RBS 2618..2622 /label=RBS misc_feature 2611..2625 /label=BBa_B0030 RBS 2611..2625 /label=BBa_B0030 CDS 2632..3306 /label=mrfp1 misc_feature 3313..3337 /label=Help:Barcodes CDS 2632..3337 /label=BBa_E1010 stem_loop 3357..3400 /label=stem_loop misc_feature 3346..3425 /label=BBa_B0010 terminator 3346..3425 /label=BBa_B0010 stem_loop 3441..3460 /label=T7 TE misc_feature 3467..3467 /label=stop polyA_site 3461..3474 /label=polya misc_feature 3434..3474 /label=BBa_B0012 terminator 3434..3474 /label=BBa_B0012 misc_feature 2393..3474 /label=BBa_K1014011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaaata ctagatgtct accgacatcg acaacgacgt tctgacctgc 121 tgcaacgtta ccgctctgtc tgctggtcac cagatcgtta tgaactctgt tctgatggac 181 atggacctgc tgctgtgcgg tgaaaccggt accggtaaag acaccctggc ttctcgtatc 241 cacgaactgt cttctcgtac cggtccgttc gttggtatga actgcgctgc tatcccggaa 301 tctctggctg aatctcagct gttcggtgtt gttaacggtg cttttactgg tgtctgccgt 361 gctcgtgaag gttacatcga agcttcttct ggtggtaccc tgtacctgga cgaaatcgac 421 tctatgccgc tgtctctgca agctaaactg ctgcgtgttc tggaatctcg tggtgttgaa 481 cgtctgggtt ctaccgactt catcccgctg gacctgcgtg ttatcgcttc tgctcagcgt 541 ccgctggacg aactggttga acagggtctg ttccgtcgtg acctgttctt ccgtctgaac 601 gttctgaccc tgcaactgcc ggctctgcgt aaacgtcgtg aacagatcct gccgctgttc 661 gaccagttca cccaggacgt tgctgctgaa tctggtcgtt ctgttccgac cctggacaac 721 cgtcgtgttc agatcctgct gtctcacgac tggccgggta acgttcgtga actgaaatct 781 gctgctaaac gtttcgttct gggtctgccg ctgctgggtg ctgaaccggt tgaagctcgt 841 gacccggtta ccggtctgcg tatgcagatg cgtgttatcg aaaaaatgct gatccaggac 901 gctctgaaac gtcaccgtca caacttcgac gctgttctgg aagaactgga actgccgcgt 961 cgtaccctgt accaccgtat gaaagaactg ggtgttgctt ctcacatcga cctggttgct 1021 gaatcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1081 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1141 accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga tagagattga 1201 catccctatc agtgatagag atactgagca ctactagaga ttaaagagga gaaatactag 1261 atgaccatcc gtaactctaa cgaatctgtt cagtctctgc actgcgcttc tcacgaatct 1321 tctctgaccc acccgtctga agacatccac ggttctctgt cttctctgat ccacaccatc 1381 gctccgctga acgttgacat cgttctggaa ggtgaaaccg gtaccggtaa agacaccctg 1441 gctcgtcgta tccaccagct gtctggttgc gctggtccgc tggttgctgt taactgcgct 1501 gctgttccgg aaaccctggc tgaatctgaa ctgttcggtg ttgtttctgg tgcttacacc 1561 ggtgcttctc gttctcgtgc tggttacctg gaaaccgctg acaaaggtat cctgttcctg 1621 gacgaaatcg actctatgcc gctgaccctg caagctaaaa tgctgcgtgt tctggaatct 1681 cgtggtatcg aacgtctggg ttctacccag ttcaaaccgg ttgacatgcg tgttatcgtt 1741 gctacccaga ccccgctgca aaaactggtt gacgaaggtc gtttccgtcg tgacctgtac 1801 ttccgtctgg acaccgttaa aatccagctg ccgaccctgc gttctcgtac cgaaatcatc 1861 ctgccgctgt tcgaacgttt cctgctggaa gcttctgctc gtctgaaacg tccggctccg 1921 ggtctgtctg ctatcatcca ggaacagctg ctgatgcacg gttggccggg taacatccgt 1981 gaactgaaag ctgctgctga acgttgggtt ctgggtctgt ctccggttcc gatcgcttct 2041 gacggtgacg ttcaggttat gggtgaagac accccgctga cctctctgaa agttcgtctg 2101 cgtcgtatag agaggttcct gatccaggag gctctgcaac gtaacgacca ctgcatcgac 2161 accgttgtta acgaactggg tatcccgaaa cgtaccctgt accaccgtat caaactgctg 2221 aacgttgctg ctcgtggtct gtaataatac tagagccagg catcaaataa aacgaaaggc 2281 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2341 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag aggccggatt 2401 atgtccgctg agtgggtcac ggtcccggat cagttccctt gcgaagctga ccgatgtttt 2461 tgatgccaaa agctgttgtg gcaaaaaacg gtttgcgcaa agttttgtat tacaaagaat 2521 ttcacatttt aaatatatct ttataaatca atcagttatt tctattttta agctggcatg 2581 gttatcgcta tagggcttgt actactagag attaaagagg agaaatacta gatggcttcc 2641 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac 2701 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc 2761 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg 2821 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg 2881 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt 2941 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa 3001 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg 3061 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg 3121 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct 3181 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc 3241 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc 3301 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 3361 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 3421 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //