##gff-version 3
##sequence-region BBa_K1014014 1 3474
BBa_K1014014	.	engineered_region	1178	2384	.	+	0	ID=BBa_K1014005;Name=BBa_K1014005
BBa_K1014014	.	terminator	2256	2335	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014005
BBa_K1014014	.	stem_loop	2267	2310	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014014	.	engineered_region	2256	2335	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014014	.	ribosome_entry_site	1240	1254	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1014005
BBa_K1014014	.	ribosome_entry_site	1240	1254	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1014014	.	ribosome_entry_site	1247	1251	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1014014	.	engineered_region	1240	1254	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1014014	.	CDS	1261	2247	.	+	0	ID=BBa_K1014000;Name=BBa_K1014000;Parent=BBa_K1014005
BBa_K1014014	.	terminator	2344	2384	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014005
BBa_K1014014	.	polyA_site	2371	2384	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014014	.	engineered_region	2344	2384	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014014	.	stem_loop	2351	2370	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014014	.	stop_codon	2377	2377	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1014014	.	promoter	1178	1231	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1014005
BBa_K1014014	.	engineered_region	1178	1231	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1014014	.	non_covalent_binding_site	1178	1196	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1014014	.	non_covalent_binding_site	1203	1221	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1014014	.	promoter	1197	1202	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1014014	.	promoter	1220	1225	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1014014	.	engineered_region	1	1169	.	+	0	ID=BBa_K1014003;Name=BBa_K1014003
BBa_K1014014	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K1014003
BBa_K1014014	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1014014	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1014014	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1014014	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1014014	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1014014	.	terminator	1129	1169	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014003
BBa_K1014014	.	polyA_site	1156	1169	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014014	.	engineered_region	1129	1169	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014014	.	stem_loop	1136	1155	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014014	.	stop_codon	1162	1162	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1014014	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1014003
BBa_K1014014	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1014014	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1014014	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1014014	.	CDS	85	1032	.	+	0	ID=BBa_K1014001;Name=BBa_K1014001;Parent=BBa_K1014003
BBa_K1014014	.	terminator	1041	1120	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014003
BBa_K1014014	.	stem_loop	1052	1095	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014014	.	engineered_region	1041	1120	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014014	.	engineered_region	2393	3474	.	+	0	ID=BBa_K1014011;Name=BBa_K1014011
BBa_K1014014	.	ribosome_entry_site	2611	2625	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1014011
BBa_K1014014	.	ribosome_entry_site	2611	2625	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1014014	.	ribosome_entry_site	2618	2622	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1014014	.	engineered_region	2611	2625	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1014014	.	promoter	2393	2602	.	+	0	ID=BBa_K1014002;Name=BBa_K1014002;Parent=BBa_K1014011
BBa_K1014014	.	CDS	2632	3337	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K1014011
BBa_K1014014	.	CDS	2632	3306	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1014014	.	engineered_tag	3313	3337	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1014014	.	terminator	3346	3425	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014011
BBa_K1014014	.	stem_loop	3357	3400	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014014	.	engineered_region	3346	3425	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014014	.	terminator	3434	3474	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014011
BBa_K1014014	.	polyA_site	3461	3474	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014014	.	engineered_region	3434	3474	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014014	.	stem_loop	3441	3460	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014014	.	stop_codon	3467	3467	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1014014
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaat
actagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgt
tatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgt
atccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctc
agctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtgg
taccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggt
gttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaac
tggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaa
acgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgacc
ctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaac
gtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagat
gcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactg
gaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatctt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatc
agtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatga
ccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaaga
catccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggt
accggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctg
ctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgc
tggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaa
atgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcg
ttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgt
taaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttct
gctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaaca
tccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttca
ggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggct
ctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatca
aactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagaggccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctg
accgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacatt
ttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactacta
gagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatgg
aaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaa
actgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagct
tacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatga
acttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaact
gcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatg
tacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaag
ttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctc
ccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctga
tagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata