##gff-version 3
##sequence-region BBa_K1014015 1 4540
BBa_K1014015	.	engineered_region	2246	3327	.	+	0	ID=BBa_K1014011;Name=BBa_K1014011
BBa_K1014015	.	terminator	3199	3278	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014011
BBa_K1014015	.	engineered_region	3199	3278	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014015	.	stem_loop	3210	3253	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014015	.	ribosome_entry_site	2464	2478	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1014011
BBa_K1014015	.	ribosome_entry_site	2471	2475	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1014015	.	engineered_region	2464	2478	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1014015	.	ribosome_entry_site	2464	2478	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1014015	.	CDS	2485	3190	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K1014011
BBa_K1014015	.	CDS	2485	3159	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1014015	.	engineered_tag	3166	3190	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1014015	.	terminator	3287	3327	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014011
BBa_K1014015	.	engineered_region	3287	3327	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014015	.	stem_loop	3294	3313	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014015	.	polyA_site	3314	3327	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014015	.	stop_codon	3320	3320	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1014015	.	promoter	2246	2455	.	+	0	ID=BBa_K1014002;Name=BBa_K1014002;Parent=BBa_K1014011
BBa_K1014015	.	engineered_region	3336	4540	.	+	0	ID=BBa_K1014006;Name=BBa_K1014006
BBa_K1014015	.	terminator	4500	4540	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014006
BBa_K1014015	.	engineered_region	4500	4540	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014015	.	stem_loop	4507	4526	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014015	.	polyA_site	4527	4540	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014015	.	stop_codon	4533	4533	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1014015	.	CDS	3417	4403	.	+	0	ID=BBa_K1014000;Name=BBa_K1014000;Parent=BBa_K1014006
BBa_K1014015	.	promoter	3336	3389	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K1014006
BBa_K1014015	.	promoter	3378	3383	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1014015	.	promoter	3355	3360	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1014015	.	engineered_region	3336	3389	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1014015	.	non_covalent_binding_site	3361	3379	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1014015	.	non_covalent_binding_site	3336	3354	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1014015	.	ribosome_entry_site	3398	3410	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K1014006
BBa_K1014015	.	ribosome_entry_site	3398	3410	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1014015	.	engineered_region	3398	3410	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1014015	.	ribosome_entry_site	3404	3407	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1014015	.	terminator	4412	4491	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014006
BBa_K1014015	.	engineered_region	4412	4491	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014015	.	stem_loop	4423	4466	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014015	.	engineered_region	1178	2237	.	+	0	ID=BBa_K1014010;Name=BBa_K1014010
BBa_K1014015	.	promoter	1178	1387	.	+	0	ID=BBa_K1014002;Name=BBa_K1014002;Parent=BBa_K1014010
BBa_K1014015	.	CDS	1416	2100	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K1014010
BBa_K1014015	.	CDS	1419	2035	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1014015	.	engineered_tag	2076	2100	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1014015	.	tag	2036	2069	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1014015	.	terminator	2109	2188	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014010
BBa_K1014015	.	engineered_region	2109	2188	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014015	.	stem_loop	2120	2163	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014015	.	terminator	2197	2237	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014010
BBa_K1014015	.	engineered_region	2197	2237	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014015	.	stem_loop	2204	2223	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014015	.	polyA_site	2224	2237	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014015	.	stop_codon	2230	2230	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1014015	.	ribosome_entry_site	1396	1409	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_K1014010
BBa_K1014015	.	conserved_region	1402	1405	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1014015	.	engineered_region	1	1169	.	+	0	ID=BBa_K1014003;Name=BBa_K1014003
BBa_K1014015	.	terminator	1041	1120	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K1014003
BBa_K1014015	.	engineered_region	1041	1120	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1014015	.	stem_loop	1052	1095	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1014015	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1014003
BBa_K1014015	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1014015	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1014015	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1014015	.	CDS	85	1032	.	+	0	ID=BBa_K1014001;Name=BBa_K1014001;Parent=BBa_K1014003
BBa_K1014015	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K1014003
BBa_K1014015	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1014015	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1014015	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1014015	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1014015	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1014015	.	terminator	1129	1169	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K1014003
BBa_K1014015	.	engineered_region	1129	1169	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1014015	.	stem_loop	1136	1155	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1014015	.	polyA_site	1156	1169	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1014015	.	stop_codon	1162	1162	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K1014015
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaat
actagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgt
tatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgt
atccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctc
agctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtgg
taccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggt
gttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaac
tggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaa
acgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgacc
ctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaac
gtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagat
gcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactg
gaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatctt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggccggatt
atgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgt
ggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagtta
tttctatttttaagctggcatggttatcgctatagggcttgtactactagagtcacacaggaaacctactagatgtcca
gattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaa
actcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcctta
gccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaata
acgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaa
acagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactc
agcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacac
ctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttctt
attcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaac
tacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggc
tcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagaggccggattatgtccgctgagtgggtcacggtcccggatcagttccc
ttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaag
aatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggc
ttgtactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaa
agttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacc
cagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacg
gttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatggga
acgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctac
aaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttcca
ccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcacta
cgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactg
gacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctt
aataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagag
tcacacaggaaagtactagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatct
tctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgaca
tcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtcc
gctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttac
accggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactcta
tgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaa
accggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgac
ctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcg
aacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgct
gatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccg
atcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatag
agaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaa
acgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttata