##gff-version 3 ##sequence-region BBa_K1014016 1 1207 BBa_K1014016 . ribosome_entry_site 63 77 . + 0 ID=BBa_B0030;Name=BBa_B0030 BBa_K1014016 . ribosome_entry_site 70 74 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K1014016 . ribosome_entry_site 63 77 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K1014016 . engineered_region 63 77 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K1014016 . terminator 1167 1207 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K1014016 . polyA_site 1194 1207 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1014016 . stem_loop 1174 1193 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1014016 . stop_codon 1200 1200 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1014016 . engineered_region 1167 1207 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1014016 . CDS 84 1070 . + 0 ID=BBa_K1014000;Name=BBa_K1014000 BBa_K1014016 . terminator 1079 1158 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_K1014016 . stem_loop 1090 1133 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1014016 . engineered_region 1079 1158 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1014016 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_K1014016 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_K1014016 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_K1014016 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_K1014016 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_K1014016 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 >BBa_K1014016 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata ctagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccg tctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtg aaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaa ctgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgt tctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgc aagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcg tgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctg gacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctgg aagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggcc gggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggt gacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatcc aggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtacca ccgtatcaaactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaataaaacgaaaggctca gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg ggtgggcctttctgcgtttata