LOCUS BBa_K1014999 4540 bp DNA linear 09-Jun-2024 DEFINITION Biological proportional operational Mu-circuit(1) ACCESSION BBa_K1014999 VERSION BBa_K1014999.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 CDS 85..1032 /label=BBa_K1014001 stem_loop 1052..1095 /label=stem_loop misc_feature 1041..1120 /label=BBa_B0010 terminator 1041..1120 /label=BBa_B0010 stem_loop 1136..1155 /label=T7 TE misc_feature 1162..1162 /label=stop polyA_site 1156..1169 /label=polya misc_feature 1129..1169 /label=BBa_B0012 terminator 1129..1169 /label=BBa_B0012 misc_feature 1..1169 /label=BBa_K1014003 promoter 1178..1387 /label=BBa_K1014002 misc_feature 1402..1405 /label=conserved RBS 1396..1409 /label=BBa_B0031 CDS 1419..2035 /label=tetR misc_feature 2036..2069 /label=SsrA misc_feature 2076..2100 /label=Help:Barcodes CDS 1416..2100 /label=BBa_C0040 stem_loop 2120..2163 /label=stem_loop misc_feature 2109..2188 /label=BBa_B0010 terminator 2109..2188 /label=BBa_B0010 stem_loop 2204..2223 /label=T7 TE misc_feature 2230..2230 /label=stop polyA_site 2224..2237 /label=polya misc_feature 2197..2237 /label=BBa_B0012 terminator 2197..2237 /label=BBa_B0012 misc_feature 1178..2237 /label=BBa_K1014010 promoter 2246..2455 /label=BBa_K1014002 RBS 2464..2478 /label=RBS-1\Strong RBS 2471..2475 /label=RBS misc_feature 2464..2478 /label=BBa_B0030 RBS 2464..2478 /label=BBa_B0030 CDS 2485..3159 /label=mrfp1 misc_feature 3166..3190 /label=Help:Barcodes CDS 2485..3190 /label=BBa_E1010 stem_loop 3210..3253 /label=stem_loop misc_feature 3199..3278 /label=BBa_B0010 terminator 3199..3278 /label=BBa_B0010 stem_loop 3294..3313 /label=T7 TE misc_feature 3320..3320 /label=stop polyA_site 3314..3327 /label=polya misc_feature 3287..3327 /label=BBa_B0012 terminator 3287..3327 /label=BBa_B0012 misc_feature 2246..3327 /label=BBa_K1014011 misc_feature 3336..3354 /label=TetR 1 misc_feature 3336..3389 /label=BBa_R0040 promoter 3355..3360 /label=-35 misc_feature 3361..3379 /label=TetR 2 promoter 3378..3383 /label=-10 promoter 3336..3389 /label=BBa_R0040 RBS 3398..3410 /label=RBS-3\Weak RBS 3404..3407 /label=RBS misc_feature 3398..3410 /label=BBa_B0032 RBS 3398..3410 /label=BBa_B0032 CDS 3417..4403 /label=BBa_K1014000 stem_loop 4423..4466 /label=stem_loop misc_feature 4412..4491 /label=BBa_B0010 terminator 4412..4491 /label=BBa_B0010 stem_loop 4507..4526 /label=T7 TE misc_feature 4533..4533 /label=stop polyA_site 4527..4540 /label=polya misc_feature 4500..4540 /label=BBa_B0012 terminator 4500..4540 /label=BBa_B0012 misc_feature 3336..4540 /label=BBa_K1014006 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaaata ctagatgtct accgacatcg acaacgacgt tctgacctgc 121 tgcaacgtta ccgctctgtc tgctggtcac cagatcgtta tgaactctgt tctgatggac 181 atggacctgc tgctgtgcgg tgaaaccggt accggtaaag acaccctggc ttctcgtatc 241 cacgaactgt cttctcgtac cggtccgttc gttggtatga actgcgctgc tatcccggaa 301 tctctggctg aatctcagct gttcggtgtt gttaacggtg cttttactgg tgtctgccgt 361 gctcgtgaag gttacatcga agcttcttct ggtggtaccc tgtacctgga cgaaatcgac 421 tctatgccgc tgtctctgca agctaaactg ctgcgtgttc tggaatctcg tggtgttgaa 481 cgtctgggtt ctaccgactt catcccgctg gacctgcgtg ttatcgcttc tgctcagcgt 541 ccgctggacg aactggttga acagggtctg ttccgtcgtg acctgttctt ccgtctgaac 601 gttctgaccc tgcaactgcc ggctctgcgt aaacgtcgtg aacagatcct gccgctgttc 661 gaccagttca cccaggacgt tgctgctgaa tctggtcgtt ctgttccgac cctggacaac 721 cgtcgtgttc agatcctgct gtctcacgac tggccgggta acgttcgtga actgaaatct 781 gctgctaaac gtttcgttct gggtctgccg ctgctgggtg ctgaaccggt tgaagctcgt 841 gacccggtta ccggtctgcg tatgcagatg cgtgttatcg aaaaaatgct gatccaggac 901 gctctgaaac gtcaccgtca caacttcgac gctgttctgg aagaactgga actgccgcgt 961 cgtaccctgt accaccgtat gaaagaactg ggtgttgctt ctcacatcga cctggttgct 1021 gaatcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1081 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1141 accttcgggt gggcctttct gcgtttatat actagaggcc ggattatgtc cgctgagtgg 1201 gtcacggtcc cggatcagtt cccttgcgaa gctgaccgat gtttttgatg ccaaaagctg 1261 ttgtggcaaa aaacggtttg cgcaaagttt tgtattacaa agaatttcac attttaaata 1321 tatctttata aatcaatcag ttatttctat ttttaagctg gcatggttat cgctataggg 1381 cttgtactac tagagtcaca caggaaacct actagatgtc cagattagat aaaagtaaag 1441 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 1501 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 1561 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 1621 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 1681 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 1741 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 1801 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 1861 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 1921 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 1981 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 2041 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 2101 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2161 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2221 gcctttctgc gtttatatac tagaggccgg attatgtccg ctgagtgggt cacggtcccg 2281 gatcagttcc cttgcgaagc tgaccgatgt ttttgatgcc aaaagctgtt gtggcaaaaa 2341 acggtttgcg caaagttttg tattacaaag aatttcacat tttaaatata tctttataaa 2401 tcaatcagtt atttctattt ttaagctggc atggttatcg ctatagggct tgtactacta 2461 gagattaaag aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg 2521 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa 2581 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt 2641 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac 2701 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa 2761 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc 2821 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac 2881 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg 2941 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac 3001 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct 3061 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa 3121 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag 3181 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 3241 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 3301 cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca 3361 tccctatcag tgatagagat actgagcact actagagtca cacaggaaag tactagatga 3421 ccatccgtaa ctctaacgaa tctgttcagt ctctgcactg cgcttctcac gaatcttctc 3481 tgacccaccc gtctgaagac atccacggtt ctctgtcttc tctgatccac accatcgctc 3541 cgctgaacgt tgacatcgtt ctggaaggtg aaaccggtac cggtaaagac accctggctc 3601 gtcgtatcca ccagctgtct ggttgcgctg gtccgctggt tgctgttaac tgcgctgctg 3661 ttccggaaac cctggctgaa tctgaactgt tcggtgttgt ttctggtgct tacaccggtg 3721 cttctcgttc tcgtgctggt tacctggaaa ccgctgacaa aggtatcctg ttcctggacg 3781 aaatcgactc tatgccgctg accctgcaag ctaaaatgct gcgtgttctg gaatctcgtg 3841 gtatcgaacg tctgggttct acccagttca aaccggttga catgcgtgtt atcgttgcta 3901 cccagacccc gctgcaaaaa ctggttgacg aaggtcgttt ccgtcgtgac ctgtacttcc 3961 gtctggacac cgttaaaatc cagctgccga ccctgcgttc tcgtaccgaa atcatcctgc 4021 cgctgttcga acgtttcctg ctggaagctt ctgctcgtct gaaacgtccg gctccgggtc 4081 tgtctgctat catccaggaa cagctgctga tgcacggttg gccgggtaac atccgtgaac 4141 tgaaagctgc tgctgaacgt tgggttctgg gtctgtctcc ggttccgatc gcttctgacg 4201 gtgacgttca ggttatgggt gaagacaccc cgctgacctc tctgaaagtt cgtctgcgtc 4261 gtatagagag gttcctgatc caggaggctc tgcaacgtaa cgaccactgc atcgacaccg 4321 ttgttaacga actgggtatc ccgaaacgta ccctgtacca ccgtatcaaa ctgctgaacg 4381 ttgctgctcg tggtctgtaa taatactaga gccaggcatc aaataaaacg aaaggctcag 4441 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 4501 cacactggct caccttcggg tgggcctttc tgcgtttata //