BBa_K1014999 1 BBa_K1014999 Biological proportional operational Mu-circuit(1) 2013-09-01T11:00:00Z 2015-05-08T01:08:42Z E.coli Biological proportional operational Mu-circuit(1) false false _999_ 0 11931 9 It's complicated false Jcat false Xiangmiao Zeng component2378511 1 BBa_K1014010 component2378541 1 BBa_K1014006 component2378497 1 BBa_K1014003 component2378525 1 BBa_K1014011 annotation2378497 1 BBa_K1014003 range2378497 1 1 1169 annotation2378541 1 BBa_K1014006 range2378541 1 3336 4540 annotation2378525 1 BBa_K1014011 range2378525 1 2246 3327 annotation2378511 1 BBa_K1014010 range2378511 1 1178 2237 BBa_K1014003 1 BBa_K1014003 HrpR Protein Generator(S) 2013-08-28T11:00:00Z 2015-05-08T01:08:42Z E.coli This device will produce HrpR protein treated with IPTG under strong RBS. false false _999_1320_ 0 11931 9 It's complicated false JCAT false Xiangmiao Zeng component2333378 1 BBa_B0012 component2333376 1 BBa_B0010 component2333373 1 BBa_B0030 component2333375 1 BBa_K1014001 component2333367 1 BBa_R0011 annotation2333378 1 BBa_B0012 range2333378 1 1129 1169 annotation2333375 1 BBa_K1014001 range2333375 1 85 1032 annotation2333376 1 BBa_B0010 range2333376 1 1041 1120 annotation2333367 1 BBa_R0011 range2333367 1 1 54 annotation2333373 1 BBa_B0030 range2333373 1 64 78 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_B0031 1 BBa_B0031 RBS.2 (weak) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false <P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-1&quot; in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Cho</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23316 1 conserved range23316 1 7 10 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1014002 1 BBa_K1014002 Promoter hrpL 2013-08-27T11:00:00Z 2015-05-08T01:08:42Z E.coli This promoter will be active,when both HrpS and HrpR are present. false false _999_1320_ 0 11931 9 In stock false n/a false Xiangmiao Zeng BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1702 1 RBS range1702 1 8 12 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_K1014000 1 BBa_K1014000 HrpS Gene 2013-08-27T11:00:00Z 2015-05-08T01:08:42Z E.coli This codes for HrpS protein which is needed for hrpL AND Gate false false _1320_ 0 11931 9 In stock false JCAT false Xiangmiao Zeng BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_K1014001 1 BBa_K1014001 HrpR Gene 2013-08-26T11:00:00Z 2015-05-08T01:08:42Z E.coli This codes for HrpR protein which is needed for hrpL AND Gate false false _999_1320_ 0 11931 9 In stock false ATGTCTACCGACATCGACAACGACGTTCTGACCTGCTGCAACGTTACCGCTCTGTCTGCTGGTCACCAGATCGTTATGAACTCTGTTCTGATGGACATGGACCTGCTGCTGTGCGGTGAAACCGGTACCGGTAAAGACACCCTGGCTTCTCGTATCCACGAACTGTCTTCTCGTACCGGTCCGTTCGTTGGTATGAACTGCGCTGCTATCCCGGAATCTCTGGCTGAATCTCAGCTGTTCGGTGTTGTTAACGGTGCTTTTACTGGTGTCTGCCGTGCTCGTGAAGGTTACATCGAAGCTTCTTCTGGTGGTACCCTGTACCTGGACGAAATCGACTCTATGCCGCTGTCTCTGCAAGCTAAACTGCTGCGTGTTCTGGAATCTCGTGGTGTTGAACGTCTGGGTTCTACCGACTTCATCCCGCTGGACCTGCGTGTTATCGCTTCTGCTCAGCGTCCGCTGGACGAACTGGTTGAACAGGGTCTGTTCCGTCGTGACCTGTTCTTCCGTCTGAACGTTCTGACCCTGCAACTGCCGGCTCTGCGTAAACGTCGTGAACAGATCCTGCCGCTGTTCGACCAGTTCACCCAGGACGTTGCTGCTGAATCTGGTCGTTCTGTTCCGACCCTGGACAACCGTCGTGTTCAGATCCTGCTGTCTCACGACTGGCCGGGTAACGTTCGTGAACTGAAATCTGCTGCTAAACGTTTCGTTCTGGGTCTGCCGCTGCTGGGTGCTGAACCGGTTGAAGCTCGTGACCCGGTTACCGGTCTGCGTATGCAGATGCGTGTTATCGAAAAAATGCTGATCCAGGACGCTCTGAAACGTCACCGTCACAACTTCGACGCTGTTCTGGAAGAACTGGAACTGCCGCGTCGTACCCTGTACCACCGTATGAAAGAACTGGGTGTTGCTTCTCACATCGACCTGGTTGCTGAATCTTAATAA false Xiangmiao Zeng BBa_K1014011 1 BBa_K1014011 RFP Protein Generator(S) 2013-08-28T11:00:00Z 2015-05-08T01:08:42Z E.coli This device will produce RFP protein when hrpL promoter is on under medium RBS. false false _999_1320_ 0 11931 9 It's complicated false JCAT false Xiangmiao Zeng component2333514 1 BBa_K1014002 component2333521 1 BBa_B0010 component2333520 1 BBa_E1010 component2333516 1 BBa_B0030 component2333523 1 BBa_B0012 annotation2333514 1 BBa_K1014002 range2333514 1 1 210 annotation2333516 1 BBa_B0030 range2333516 1 219 233 annotation2333520 1 BBa_E1010 range2333520 1 240 945 annotation2333523 1 BBa_B0012 range2333523 1 1042 1082 annotation2333521 1 BBa_B0010 range2333521 1 954 1033 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_K1014006 1 BBa_K1014006 HrpS Protein Generator(M) 2013-08-28T11:00:00Z 2015-05-08T01:08:42Z E.coli This device will produce HrpS protein constitutively under medium RBS and is repressed by TetR. false false _999_1320_ 0 11931 9 In stock false JCAT false Xiangmiao Zeng component2334784 1 BBa_B0032 component2334787 1 BBa_B0010 component2334778 1 BBa_R0040 component2334786 1 BBa_K1014000 component2334789 1 BBa_B0012 annotation2334784 1 BBa_B0032 range2334784 1 63 75 annotation2334778 1 BBa_R0040 range2334778 1 1 54 annotation2334786 1 BBa_K1014000 range2334786 1 82 1068 annotation2334787 1 BBa_B0010 range2334787 1 1077 1156 annotation2334789 1 BBa_B0012 range2334789 1 1165 1205 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1014010 1 BBa_K1014010 tetR Protein Generator(W) 2013-08-28T11:00:00Z 2015-05-08T01:08:42Z E.coli This device will produce tetR protein when hrpL promoter is on under weak RBS. false false _999_1320_ 0 11931 9 In stock false JCAT false Xiangmiao Zeng component2334827 1 BBa_C0040 component2334830 1 BBa_B0012 component2334821 1 BBa_K1014002 component2334828 1 BBa_B0010 component2334823 1 BBa_B0031 annotation2334823 1 BBa_B0031 range2334823 1 219 232 annotation2334830 1 BBa_B0012 range2334830 1 1020 1060 annotation2334827 1 BBa_C0040 range2334827 1 239 923 annotation2334828 1 BBa_B0010 range2334828 1 932 1011 annotation2334821 1 BBa_K1014002 range2334821 1 1 210 BBa_C0040 1 tetR tetracycline repressor from transposon Tn10 (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999) Released HQ 2013 Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.</P> false true _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P> References (unparsed) here: <p>Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). </P> <p> Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 </p> <P>BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman. annotation23329 1 tetR range23329 1 4 620 annotation2213989 1 Help:Barcodes range2213989 1 661 685 annotation23330 1 SsrA range23330 1 621 654 BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2001 1 lac O1 range2001 1 26 42 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 BBa_K1014001_sequence 1 atgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K1014003_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1014000_sequence 1 atgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataa BBa_K1014011_sequence 1 gccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1014002_sequence 1 gccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtac BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1014006_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1014010_sequence 1 gccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0030_sequence 1 attaaagaggagaaa BBa_B0032_sequence 1 tcacacaggaaag BBa_K1014999_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgtctaccgacatcgacaacgacgttctgacctgctgcaacgttaccgctctgtctgctggtcaccagatcgttatgaactctgttctgatggacatggacctgctgctgtgcggtgaaaccggtaccggtaaagacaccctggcttctcgtatccacgaactgtcttctcgtaccggtccgttcgttggtatgaactgcgctgctatcccggaatctctggctgaatctcagctgttcggtgttgttaacggtgcttttactggtgtctgccgtgctcgtgaaggttacatcgaagcttcttctggtggtaccctgtacctggacgaaatcgactctatgccgctgtctctgcaagctaaactgctgcgtgttctggaatctcgtggtgttgaacgtctgggttctaccgacttcatcccgctggacctgcgtgttatcgcttctgctcagcgtccgctggacgaactggttgaacagggtctgttccgtcgtgacctgttcttccgtctgaacgttctgaccctgcaactgccggctctgcgtaaacgtcgtgaacagatcctgccgctgttcgaccagttcacccaggacgttgctgctgaatctggtcgttctgttccgaccctggacaaccgtcgtgttcagatcctgctgtctcacgactggccgggtaacgttcgtgaactgaaatctgctgctaaacgtttcgttctgggtctgccgctgctgggtgctgaaccggttgaagctcgtgacccggttaccggtctgcgtatgcagatgcgtgttatcgaaaaaatgctgatccaggacgctctgaaacgtcaccgtcacaacttcgacgctgttctggaagaactggaactgccgcgtcgtaccctgtaccaccgtatgaaagaactgggtgttgcttctcacatcgacctggttgctgaatcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggccggattatgtccgctgagtgggtcacggtcccggatcagttcccttgcgaagctgaccgatgtttttgatgccaaaagctgttgtggcaaaaaacggtttgcgcaaagttttgtattacaaagaatttcacattttaaatatatctttataaatcaatcagttatttctatttttaagctggcatggttatcgctatagggcttgtactactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatgaccatccgtaactctaacgaatctgttcagtctctgcactgcgcttctcacgaatcttctctgacccacccgtctgaagacatccacggttctctgtcttctctgatccacaccatcgctccgctgaacgttgacatcgttctggaaggtgaaaccggtaccggtaaagacaccctggctcgtcgtatccaccagctgtctggttgcgctggtccgctggttgctgttaactgcgctgctgttccggaaaccctggctgaatctgaactgttcggtgttgtttctggtgcttacaccggtgcttctcgttctcgtgctggttacctggaaaccgctgacaaaggtatcctgttcctggacgaaatcgactctatgccgctgaccctgcaagctaaaatgctgcgtgttctggaatctcgtggtatcgaacgtctgggttctacccagttcaaaccggttgacatgcgtgttatcgttgctacccagaccccgctgcaaaaactggttgacgaaggtcgtttccgtcgtgacctgtacttccgtctggacaccgttaaaatccagctgccgaccctgcgttctcgtaccgaaatcatcctgccgctgttcgaacgtttcctgctggaagcttctgctcgtctgaaacgtccggctccgggtctgtctgctatcatccaggaacagctgctgatgcacggttggccgggtaacatccgtgaactgaaagctgctgctgaacgttgggttctgggtctgtctccggttccgatcgcttctgacggtgacgttcaggttatgggtgaagacaccccgctgacctctctgaaagttcgtctgcgtcgtatagagaggttcctgatccaggaggctctgcaacgtaacgaccactgcatcgacaccgttgttaacgaactgggtatcccgaaacgtaccctgtaccaccgtatcaaactgctgaacgttgctgctcgtggtctgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0040_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac BBa_B0031_sequence 1 tcacacaggaaacc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z