LOCUS       BBa_K1015013            1011 bp    DNA     linear       26-Nov-2024
DEFINITION  Km-Blunt end
ACCESSION   BBa_K1015013
VERSION     BBa_K1015013.1  
FEATURES             Location/Qualifiers
     misc_feature    2..7
                     /label=Pvu2 restriction site
     misc_feature    9..14
                     /label=Nru1 restriction site
     misc_feature    15..20
                     /label=Sma1 restriction site
     CDS             125..937
                     /label=Km resistant gene
     misc_feature    1005..1010
                     /label=Pvu2 restriction site
     misc_feature    998..1003
                     /label=Nru1 restriction site
     misc_feature    992..997
                     /label=Sma1 restriction site
ORIGIN
        1 ccagctgctc gcgacccggg cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
       61 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
      121 gtgttatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta aattccaaca
      181 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
      241 caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
      301 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
      361 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
      421 ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
      481 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
      541 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
      601 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
      661 ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact catggtgatt
      721 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
      781 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
      841 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
      901 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
      961 tgtaacactg gcagagcatt acgctgactt gcccgggtcg cgagcagctg g
//