BBa_K1015013 1 BBa_K1015013 Km-Blunt end 2013-09-15T11:00:00Z 2015-05-08T01:08:42Z pACYC177 This part changes cohesive end to blunt end on the prefix and suffix. Also this includes a gene which has resistance to kanamycin. Therefore it works as an antibiotic resistance marker to select colonies of E.coli which succeeded. false false _1321_ 0 15618 9 It's complicated true This part can insert other part easily between backbone. false Kento Tominaga annotation2357357 1 Pvu2 restriction site range2357357 1 2 7 annotation2357361 1 Pvu2 restriction site range2357361 1 1005 1010 annotation2357359 1 Sma1 restriction site range2357359 1 15 20 annotation2357360 1 Km resistant gene range2357360 1 125 937 annotation2357362 1 Nru1 restriction site range2357362 1 998 1003 annotation2357367 1 Sma1 restriction site range2357367 1 992 997 annotation2357358 1 Nru1 restriction site range2357358 1 9 14 BBa_K1015013_sequence 1 ccagctgctcgcgacccgggcacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgtaacactggcagagcattacgctgacttgcccgggtcgcgagcagctgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z