LOCUS BBa_K1015014 2761 bp DNA linear 22-Nov-2024 DEFINITION IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR) ACCESSION BBa_K1015014 VERSION BBa_K1015014.1 FEATURES Location/Qualifiers misc_feature 1..36 /label=lacA homology sequence misc_feature 1..36 /label=BBa_K1015000 misc_feature 45..61 /label=IRR out misc_feature 63..79 /label=IRR in misc_feature 45..79 /label=BBa_K137008 promoter 88..141 /label=tetR promoter promoter 88..337 /label=BBa_K137047 misc_feature 346..362 /label=IRL in misc_feature 364..380 /label=IRL out misc_feature 346..380 /label=BBa_K137010 misc_feature 393..396 /label=conserved RBS 389..400 /label=BBa_B0034 CDS 407..1678 /label=FLP CDS 407..1678 /label=BBa_K1015001 CDS 1805..2597 /label=aadA(streptomycin resistance gene) promoter 1687..1804 /label=blaZ fragment(promoter contain) misc_feature 2597..2716 /label=blaZ fragment CDS 1687..2717 /label=BBa_K1015002 misc_feature 2726..2761 /label=cynR homology sequence misc_feature 2726..2761 /label=BBa_K1015003 ORIGIN 1 cggaagtcat gtggttatta atccaggcgt caccattact agagaagatg aaacatttgg 61 ggccaaactg tccatattat actagaggtg ctcagtatct ctatcactga tagggatgtc 121 aatctctatc actgataggg actctagtat ataaacgcag aaaggcccac ccgaaggtga 181 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 241 gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagtga tctacactag 301 cactatcagt gttattaagc tactaaagcg tagtttttac tagagtctat gagtcaaaat 361 ggccccaatt gtcttgtatt tactagagaa agaggagaaa tactagatgc cacaatttgg 421 tatattatgt aaaacaccac ctaaggtgct tgttcgtcag tttgtggaaa ggtttgaaag 481 accttcaggt gagaaaatag cattatgtgc tgctgaacta acctatttat gttggatgat 541 tacacataac ggaacagcaa tcaagagagc cacattcatg agctataata ctatcataag 601 caattcgctg agtttcgata ttgtcaataa atcactccag tttaaataca agacgcaaaa 661 agcaacaatt ctggaagcct cattaaagaa attgattcct gcttgggaat ttacaattat 721 tccttactat ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt 781 acagttcgaa tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa 841 agcacttcta agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt 901 tgagtatact tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac 961 tttcatcaat tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt 1021 agtccaaaat aagtatctgg gagtaataat ccagtgttta gtgacagaga caaagacaag 1081 cgttagtagg cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt 1141 ggatgaattt ttgaggaatt ctgaaccagt cctaaaacga gtaaatagga ccggcaattc 1201 ttcaagcaat aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaataa 1261 agctttgaag aaaaatgcgc cttattcaat ctttgctata aaaaatggcc caaaatctca 1321 cattggaaga catttgatga cctcatttct ttcaatgaag ggcctaacgg agttgactaa 1381 tgttgtggga aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca 1441 tcagataaca gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga 1501 tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca 1561 gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg 1621 gataatatca caggaggtac tagactacct ttcatcctac ataaatagac gcatataata 1681 ctagagtaac ttagccattt caacaccttc tttcaaatat ttataataaa ctattgacac 1741 cgatattaca attgtaatat tattgattta taaaaattac aactgtaata tcggagggtt 1801 tatttatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg 1861 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg 1921 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg 1981 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag 2041 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt 2101 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg 2161 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa 2221 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg 2281 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact 2341 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa 2401 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc 2461 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct 2521 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca 2581 aggtagtcgg caaataaata ttctaaatgc ataataaata ctgataacat cttatatttt 2641 gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc 2701 tctattattt tcgagattac tagagtcacg gtagccgctg acacaggtct gcggctggaa 2761 t //