LOCUS       BBa_K1015014            2761 bp    DNA     linear       22-Dec-2024
DEFINITION  IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
ACCESSION   BBa_K1015014
VERSION     BBa_K1015014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..36
                     /label=lacA homology sequence
     misc_feature    1..36
                     /label=BBa_K1015000
     misc_feature    45..61
                     /label=IRR out
     misc_feature    63..79
                     /label=IRR in
     misc_feature    45..79
                     /label=BBa_K137008
     promoter        88..141
                     /label=tetR promoter
     promoter        88..337
                     /label=BBa_K137047
     misc_feature    346..362
                     /label=IRL in
     misc_feature    364..380
                     /label=IRL out
     misc_feature    346..380
                     /label=BBa_K137010
     misc_feature    393..396
                     /label=conserved
     RBS             389..400
                     /label=BBa_B0034
     CDS             407..1678
                     /label=FLP
     CDS             407..1678
                     /label=BBa_K1015001
     CDS             1805..2597
                     /label=aadA(streptomycin resistance gene)
     promoter        1687..1804
                     /label=blaZ fragment(promoter contain)
     misc_feature    2597..2716
                     /label=blaZ fragment
     CDS             1687..2717
                     /label=BBa_K1015002
     misc_feature    2726..2761
                     /label=cynR homology sequence
     misc_feature    2726..2761
                     /label=BBa_K1015003
ORIGIN
        1 cggaagtcat gtggttatta atccaggcgt caccattact agagaagatg aaacatttgg
       61 ggccaaactg tccatattat actagaggtg ctcagtatct ctatcactga tagggatgtc
      121 aatctctatc actgataggg actctagtat ataaacgcag aaaggcccac ccgaaggtga
      181 gccagtgtga ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      241 gtctttcgac tgagcctttc gttttatttg atgcctggct ctagtagtga tctacactag
      301 cactatcagt gttattaagc tactaaagcg tagtttttac tagagtctat gagtcaaaat
      361 ggccccaatt gtcttgtatt tactagagaa agaggagaaa tactagatgc cacaatttgg
      421 tatattatgt aaaacaccac ctaaggtgct tgttcgtcag tttgtggaaa ggtttgaaag
      481 accttcaggt gagaaaatag cattatgtgc tgctgaacta acctatttat gttggatgat
      541 tacacataac ggaacagcaa tcaagagagc cacattcatg agctataata ctatcataag
      601 caattcgctg agtttcgata ttgtcaataa atcactccag tttaaataca agacgcaaaa
      661 agcaacaatt ctggaagcct cattaaagaa attgattcct gcttgggaat ttacaattat
      721 tccttactat ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt
      781 acagttcgaa tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa
      841 agcacttcta agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt
      901 tgagtatact tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac
      961 tttcatcaat tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt
     1021 agtccaaaat aagtatctgg gagtaataat ccagtgttta gtgacagaga caaagacaag
     1081 cgttagtagg cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt
     1141 ggatgaattt ttgaggaatt ctgaaccagt cctaaaacga gtaaatagga ccggcaattc
     1201 ttcaagcaat aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaataa
     1261 agctttgaag aaaaatgcgc cttattcaat ctttgctata aaaaatggcc caaaatctca
     1321 cattggaaga catttgatga cctcatttct ttcaatgaag ggcctaacgg agttgactaa
     1381 tgttgtggga aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca
     1441 tcagataaca gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga
     1501 tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca
     1561 gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg
     1621 gataatatca caggaggtac tagactacct ttcatcctac ataaatagac gcatataata
     1681 ctagagtaac ttagccattt caacaccttc tttcaaatat ttataataaa ctattgacac
     1741 cgatattaca attgtaatat tattgattta taaaaattac aactgtaata tcggagggtt
     1801 tatttatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1861 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1921 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1981 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2041 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2101 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2161 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2221 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2281 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2341 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2401 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2461 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2521 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2581 aggtagtcgg caaataaata ttctaaatgc ataataaata ctgataacat cttatatttt
     2641 gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac tgattttccc
     2701 tctattattt tcgagattac tagagtcacg gtagccgctg acacaggtct gcggctggaa
     2761 t
//