BBa_K1015002 1 BBa_K1015002 streptmycin resisitance gene (can PCR ampicillin resistance gene primer) 2013-09-14T11:00:00Z 2016-01-28T01:50:11Z aminoglycoside resistance gene (aadA) come from R100.1 plasmid. streptmycin resisitance gene(aadA) This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ. false false _1321_ 4206 15618 9 Not in stock false antibiotic resistance false Kento Tominaga annotation2342881 1 blaZ fragment(promoter contain) range2342881 1 1 118 annotation2342888 1 blaZ fragment range2342888 1 911 1030 annotation2342880 1 aadA(streptomycin resistance gene) range2342880 1 119 911 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1015014 1 BBa_K1015014 IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR) 2013-09-14T11:00:00Z 2015-05-08T01:08:42Z BBa_K1015000 BBa_K137008 BBa_K137047 BBa_K137010 B0034 BBa_K1015001 BBa_K1015002 BBa_K1015003 With acteriophage &#955; recombination sysytem, this device insert into Escherichia coli genome. This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop. false false _1321_ 0 15618 9 It's complicated true genome insertion parts false Kento Tominaga component2374427 1 BBa_K137010 component2374424 1 BBa_K137047 component2374422 1 BBa_K137008 component2374429 1 BBa_B0034 component2374435 1 BBa_K1015002 component2374419 1 BBa_K1015000 component2374431 1 BBa_K1015001 component2374437 1 BBa_K1015003 annotation2374424 1 BBa_K137047 range2374424 1 88 337 annotation2374419 1 BBa_K1015000 range2374419 1 1 36 annotation2374427 1 BBa_K137010 range2374427 1 346 380 annotation2374431 1 BBa_K1015001 range2374431 1 407 1678 annotation2374422 1 BBa_K137008 range2374422 1 45 79 annotation2374435 1 BBa_K1015002 range2374435 1 1687 2717 annotation2374437 1 BBa_K1015003 range2374437 1 2726 2761 annotation2374429 1 BBa_B0034 range2374429 1 389 400 BBa_K137047 1 BBa_K137047 250 bp inverted tetR promoter 2008-07-20T11:00:00Z 2015-05-08T01:10:09Z Primers were synthesized to bind to part Q04400, and then a PCR reaction was run. Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction. false false _187_ 0 3112 9 It's complicated false This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed. false Allen Lin annotation1968020 1 tetR promoter range1968020 1 1 54 BBa_K1015000 1 BBa_K1015000 lacA-homology sequence(for RED recombination system) 2013-09-14T11:00:00Z 2015-05-08T01:08:42Z CGGAAGTCATGTGGTTATTAATCCAGGCGTCACCAT This sequence is 36bp homologous sequence with Escherichia coli lacA gene. Bacteriophage &#955; recombination system(RED system) can cause homologous recombination between this sequence and lacA gene. Because of the recombination, Biobrick part can insert into E.coli genome. false false _1321_ 0 15618 9 Not in stock false Genome insertion false Kento Tominaga annotation2343703 1 lacA homology sequence range2343703 1 1 36 BBa_K1015003 1 BBa_K1015003 cynR homology sequence(for RED recombination system) 2013-09-14T11:00:00Z 2015-05-08T01:08:42Z Escherichia coli MG1655 cynR gene This sequence is 36bp homologous sequence with Escherichia coli cynR gene. Bacteriophage &#955; recombination system(RED system) can cause homologous recombination between this sequence and cynR gene. Because of the recombination, Biobrick part can insert into E.coli genome. false false _1321_ 0 15618 9 Not in stock false Genome insertion false Kento Tominaga annotation2342941 1 cynR homology sequence range2342941 1 1 36 BBa_K137010 1 fimE IRL fimE IRL 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z pFIP plasmid fimE inverted repeat left recombination site false false _187_ 0 3112 9 It's complicated false none false Allen Lin annotation1963844 1 IRL out range1963844 1 19 35 annotation1963843 1 IRL in range1963843 1 1 17 BBa_K1015001 1 BBa_K1015001 Flippase(FLP) 2013-09-14T11:00:00Z 2015-05-08T01:08:42Z 2??m plasmid of Saccharomyces cerevisiae. site specific recombinase that recognize FRT site. FLP catalyze site specific recombination between two FRT site. false false _1321_ 0 15618 9 Not in stock false site specific recombination between two FRT site. false Kento Tominaga annotation2342868 1 FLP range2342868 1 1 1272 BBa_K137008 1 IRR fimE IRR 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z pFIP plasmid fimE inverted repeat right recombination site false false _187_ 0 3112 9 It's complicated false none false Allen Lin annotation1963842 1 IRR in range1963842 1 19 35 annotation1963841 1 IRR out range1963841 1 1 17 BBa_K137010_sequence 1 tctatgagtcaaaatggccccaattgtcttgtatt BBa_K1015003_sequence 1 tcacggtagccgctgacacaggtctgcggctggaat BBa_K137008_sequence 1 aagatgaaacatttggggccaaactgtccatatta BBa_B0034_sequence 1 aaagaggagaaa BBa_K1015001_sequence 1 atgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataa BBa_K1015014_sequence 1 cggaagtcatgtggttattaatccaggcgtcaccattactagagaagatgaaacatttggggccaaactgtccatattatactagaggtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttttactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagaaagaggagaaatactagatgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataatactagagtaacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagattactagagtcacggtagccgctgacacaggtctgcggctggaat BBa_K137047_sequence 1 gtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttt BBa_K1015000_sequence 1 cggaagtcatgtggttattaatccaggcgtcaccat BBa_K1015002_sequence 1 taacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z