BBa_K1015014
type: ComponentDefinition
persistent identity: BBa K1015014
display id: BBa_K1015014
version: 1
was generated by: iGEM to SBOL conversion
description: IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: BBa_K1015000 BBa_K137008 BBa_K137047 BBa_K137010 B0034 BBa_K1015001 BBa_K1015002 BBa_K1015003
owned by: james
owned by: myers
mutable description: With acteriophage λ recombination sysytem, this device insert into Escherichia coli genome. This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.
discontinued: false
dominant: false
experience: experience/Issues
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: genome insertion parts
star: false
creator: Kento Tominaga
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1015003
component: BBa_K1015002
component: BBa_K1015001
component: BBa_B0034
component: BBa_K137010
component: BBa_K137047
component: BBa_K137008
component: BBa_K1015000
sequence annotation: BBa_K1015000
sequence annotation: BBa_K137008
sequence annotation: BBa_K137047
sequence annotation: BBa_K137010
sequence annotation: BBa_B0034
sequence annotation: BBa_K1015001
sequence annotation: BBa_K1015002
sequence annotation: BBa_K1015003
sequence: https://synbiohub.org/public/igem/BBa_K1015014_sequence/1

BBa_K1015003
type: Component
persistent identity: component2374437
display id: component2374437
version: 1
top level: BBa_K1015014
definition: BBa_K1015003
access: public

BBa_K1015002
type: Component
persistent identity: component2374435
display id: component2374435
version: 1
top level: BBa_K1015014
definition: BBa_K1015002
access: public

BBa_K1015001
type: Component
persistent identity: component2374431
display id: component2374431
version: 1
top level: BBa_K1015014
definition: BBa_K1015001
access: public

BBa_B0034
type: Component
persistent identity: component2374429
display id: component2374429
version: 1
top level: BBa_K1015014
definition: BBa_B0034
access: public

BBa_K137010
type: Component
persistent identity: component2374427
display id: component2374427
version: 1
top level: BBa_K1015014
definition: fimE IRL
access: public

BBa_K137047
type: Component
persistent identity: component2374424
display id: component2374424
version: 1
top level: BBa_K1015014
definition: BBa_K137047
access: public

BBa_K137008
type: Component
persistent identity: component2374422
display id: component2374422
version: 1
top level: BBa_K1015014
definition: IRR
access: public

BBa_K1015000
type: Component
persistent identity: component2374419
display id: component2374419
version: 1
top level: BBa_K1015014
definition: BBa_K1015000
access: public

BBa_K1015000
type: SequenceAnnotation
persistent identity: annotation2374419
display id: annotation2374419
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374419/range2374419/1
component: BBa_K1015000

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374419/range2374419/1
type: Range
persistent identity: range2374419
display id: range2374419
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 1
end: 36
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation2374422
display id: annotation2374422
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374422/range2374422/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374422/range2374422/1
type: Range
persistent identity: range2374422
display id: range2374422
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 45
end: 79
orientation: inline

BBa_K137047
type: SequenceAnnotation
persistent identity: annotation2374424
display id: annotation2374424
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374424/range2374424/1
component: BBa_K137047

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374424/range2374424/1
type: Range
persistent identity: range2374424
display id: range2374424
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 88
end: 337
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation2374427
display id: annotation2374427
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374427/range2374427/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374427/range2374427/1
type: Range
persistent identity: range2374427
display id: range2374427
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 346
end: 380
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2374429
display id: annotation2374429
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374429/range2374429/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374429/range2374429/1
type: Range
persistent identity: range2374429
display id: range2374429
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 389
end: 400
orientation: inline

BBa_K1015001
type: SequenceAnnotation
persistent identity: annotation2374431
display id: annotation2374431
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374431/range2374431/1
component: BBa_K1015001

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374431/range2374431/1
type: Range
persistent identity: range2374431
display id: range2374431
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 407
end: 1678
orientation: inline

BBa_K1015002
type: SequenceAnnotation
persistent identity: annotation2374435
display id: annotation2374435
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374435/range2374435/1
component: BBa_K1015002

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374435/range2374435/1
type: Range
persistent identity: range2374435
display id: range2374435
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 1687
end: 2717
orientation: inline

BBa_K1015003
type: SequenceAnnotation
persistent identity: annotation2374437
display id: annotation2374437
version: 1
top level: BBa_K1015014
location: https://synbiohub.org/public/igem/BBa_K1015014/annotation2374437/range2374437/1
component: BBa_K1015003

https://synbiohub.org/public/igem/BBa_K1015014/annotation2374437/range2374437/1
type: Range
persistent identity: range2374437
display id: range2374437
version: 1
top level: BBa_K1015014
direction: direction/reverse
start: 2726
end: 2761
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

IRR
type: ComponentDefinition
persistent identity: BBa K137008
display id: BBa_K137008
version: 1
was generated by: iGEM to SBOL conversion
description: fimE IRR
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
mutable description: fimE inverted repeat right recombination site
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Allen Lin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: IRR out
sequence annotation: IRR in
sequence: https://synbiohub.org/public/igem/BBa_K137008_sequence/1

IRR out
type: SequenceAnnotation
persistent identity: annotation1963841
display id: annotation1963841
version: 1
top level: IRR
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1
type: Range
persistent identity: range1963841
display id: range1963841
version: 1
top level: IRR
direction: direction/forward
start: 1
end: 17
orientation: inline

IRR in
type: SequenceAnnotation
persistent identity: annotation1963842
display id: annotation1963842
version: 1
top level: IRR
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1
type: Range
persistent identity: range1963842
display id: range1963842
version: 1
top level: IRR
direction: direction/forward
start: 19
end: 35
orientation: inline

fimE IRL
type: ComponentDefinition
persistent identity: BBa K137010
display id: BBa_K137010
version: 1
was generated by: iGEM to SBOL conversion
description: fimE IRL
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
mutable description: fimE inverted repeat left recombination site
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Allen Lin
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: IRL in
sequence annotation: IRL out
sequence: https://synbiohub.org/public/igem/BBa_K137010_sequence/1

IRL in
type: SequenceAnnotation
persistent identity: annotation1963843
display id: annotation1963843
version: 1
top level: fimE IRL
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1
type: Range
persistent identity: range1963843
display id: range1963843
version: 1
top level: fimE IRL
direction: direction/forward
start: 1
end: 17
orientation: inline

IRL out
type: SequenceAnnotation
persistent identity: annotation1963844
display id: annotation1963844
version: 1
top level: fimE IRL
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1
type: Range
persistent identity: range1963844
display id: range1963844
version: 1
top level: fimE IRL
direction: direction/forward
start: 19
end: 35
orientation: inline

BBa_K137047
type: ComponentDefinition
persistent identity: BBa K137047
display id: BBa_K137047
version: 1
was generated by: iGEM to SBOL conversion
description: 250 bp inverted tetR promoter
created: 2008-07-20T11:00:00Z
modified: 2015-05-08T01:10:09Z
mutable provenance: Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
owned by: james
owned by: myers
mutable description: Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
discontinued: false
dominant: false
experience: experience/Works
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
star: false
creator: Allen Lin
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: tetR promoter
sequence: https://synbiohub.org/public/igem/BBa_K137047_sequence/1

tetR promoter
type: SequenceAnnotation
persistent identity: annotation1968020
display id: annotation1968020
version: 1
top level: BBa_K137047
location: https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K137047/annotation1968020/range1968020/1
type: Range
persistent identity: range1968020
display id: range1968020
version: 1
top level: BBa_K137047
direction: direction/reverse
start: 1
end: 54
orientation: inline

BBa_K1015000
type: ComponentDefinition
persistent identity: BBa K1015000
display id: BBa_K1015000
version: 1
was generated by: iGEM to SBOL conversion
description: lacA-homology sequence(for RED recombination system)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: CGGAAGTCATGTGGTTATTAATCCAGGCGTCACCAT
owned by: james
owned by: myers
mutable description: This sequence is 36bp homologous sequence with Escherichia coli lacA gene. Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and lacA gene. Because of the recombination, Biobrick part can insert into E.coli genome.
discontinued: false
dominant: false
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Genome insertion
star: false
creator: Kento Tominaga
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: lacA homology sequence
sequence: https://synbiohub.org/public/igem/BBa_K1015000_sequence/1

lacA homology sequence
type: SequenceAnnotation
persistent identity: annotation2343703
display id: annotation2343703
version: 1
top level: BBa_K1015000
location: https://synbiohub.org/public/igem/BBa_K1015000/annotation2343703/range2343703/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1015000/annotation2343703/range2343703/1
type: Range
persistent identity: range2343703
display id: range2343703
version: 1
top level: BBa_K1015000
direction: direction/forward
start: 1
end: 36
orientation: inline

BBa_K1015001
type: ComponentDefinition
persistent identity: BBa K1015001
display id: BBa_K1015001
version: 1
was generated by: iGEM to SBOL conversion
description: Flippase(FLP)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: 2??m plasmid of Saccharomyces cerevisiae.
owned by: james
owned by: myers
mutable description: site specific recombinase that recognize FRT site. FLP catalyze site specific recombination between two FRT site.
discontinued: false
dominant: false
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: site specific recombination between two FRT site.
star: false
creator: Kento Tominaga
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FLP
sequence: https://synbiohub.org/public/igem/BBa_K1015001_sequence/1

FLP
type: SequenceAnnotation
persistent identity: annotation2342868
display id: annotation2342868
version: 1
top level: BBa_K1015001
location: https://synbiohub.org/public/igem/BBa_K1015001/annotation2342868/range2342868/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1015001/annotation2342868/range2342868/1
type: Range
persistent identity: range2342868
display id: range2342868
version: 1
top level: BBa_K1015001
direction: direction/forward
start: 1
end: 1272
orientation: inline

BBa_K1015002
type: ComponentDefinition
persistent identity: BBa K1015002
display id: BBa_K1015002
version: 1
was generated by: iGEM to SBOL conversion
description: streptmycin resisitance gene (can PCR ampicillin resistance gene primer)
created: 2013-09-14T11:00:00Z
modified: 2016-01-28T01:50:11Z
mutable provenance: aminoglycoside resistance gene (aadA) come from R100.1 plasmid.
owned by: james
owned by: myers
mutable description: streptmycin resisitance gene(aadA) This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ.
discontinued: false
dominant: false
experience: experience/None
group u list: _1321_
m user id: 4206
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: antibiotic resistance
star: false
creator: Kento Tominaga
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: blaZ fragment(promoter contain)
sequence annotation: aadA(streptomycin resistance gene)
sequence annotation: blaZ fragment
sequence: https://synbiohub.org/public/igem/BBa_K1015002_sequence/1

blaZ fragment(promoter contain)
type: SequenceAnnotation
persistent identity: annotation2342881
display id: annotation2342881
version: 1
top level: BBa_K1015002
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342881/range2342881/1
type: Range
persistent identity: range2342881
display id: range2342881
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 1
end: 118
orientation: inline

aadA(streptomycin resistance gene)
type: SequenceAnnotation
persistent identity: annotation2342880
display id: annotation2342880
version: 1
top level: BBa_K1015002
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342880/range2342880/1
type: Range
persistent identity: range2342880
display id: range2342880
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 119
end: 911
orientation: inline

blaZ fragment
type: SequenceAnnotation
persistent identity: annotation2342888
display id: annotation2342888
version: 1
top level: BBa_K1015002
location: https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K1015002/annotation2342888/range2342888/1
type: Range
persistent identity: range2342888
display id: range2342888
version: 1
top level: BBa_K1015002
direction: direction/forward
start: 911
end: 1030
orientation: inline

BBa_K1015003
type: ComponentDefinition
persistent identity: BBa K1015003
display id: BBa_K1015003
version: 1
was generated by: iGEM to SBOL conversion
description: cynR homology sequence(for RED recombination system)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:08:42Z
mutable provenance: Escherichia coli MG1655 cynR gene
owned by: james
owned by: myers
mutable description: This sequence is 36bp homologous sequence with Escherichia coli cynR gene. Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and cynR gene. Because of the recombination, Biobrick part can insert into E.coli genome.
discontinued: false
dominant: false
group u list: _1321_
m user id: 0
owner id: 15618
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Genome insertion
star: false
creator: Kento Tominaga
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: cynR homology sequence
sequence: https://synbiohub.org/public/igem/BBa_K1015003_sequence/1

cynR homology sequence
type: SequenceAnnotation
persistent identity: annotation2342941
display id: annotation2342941
version: 1
top level: BBa_K1015003
location: https://synbiohub.org/public/igem/BBa_K1015003/annotation2342941/range2342941/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K1015003/annotation2342941/range2342941/1
type: Range
persistent identity: range2342941
display id: range2342941
version: 1
top level: BBa_K1015003
direction: direction/forward
start: 1
end: 36
orientation: inline

https://synbiohub.org/public/igem/BBa_K1015014_sequence/1
type: Sequence
persistent identity: BBa K1015014 sequence
display id: BBa_K1015014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggaagtcatgtggttattaatccaggcgtcaccattactagagaagatgaaacatttggggccaaactgtccatattatactagaggtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttttactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagaaagaggagaaatactagatgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataatactagagtaacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137008_sequence/1
type: Sequence
persistent identity: BBa K137008 sequence
display id: BBa_K137008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagatgaaacatttggggccaaactgtccatatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137010_sequence/1
type: Sequence
persistent identity: BBa K137010 sequence
display id: BBa_K137010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctatgagtcaaaatggccccaattgtcttgtatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137047_sequence/1
type: Sequence
persistent identity: BBa K137047 sequence
display id: BBa_K137047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagtttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015000_sequence/1
type: Sequence
persistent identity: BBa K1015000 sequence
display id: BBa_K1015000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cggaagtcatgtggttattaatccaggcgtcaccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015001_sequence/1
type: Sequence
persistent identity: BBa K1015001 sequence
display id: BBa_K1015001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccacaatttggtatattatgtaaaacaccacctaaggtgcttgttcgtcagtttgtggaaaggtttgaaagaccttcaggtgagaaaatagcattatgtgctgctgaactaacctatttatgttggatgattacacataacggaacagcaatcaagagagccacattcatgagctataatactatcataagcaattcgctgagtttcgatattgtcaataaatcactccagtttaaatacaagacgcaaaaagcaacaattctggaagcctcattaaagaaattgattcctgcttgggaatttacaattattccttactatggacaaaaacatcaatctgatatcactgatattgtaagtagtttgcaattacagttcgaatcatcggaagaagcagataagggaaatagccacagtaaaaaaatgcttaaagcacttctaagtgagggtgaaagcatctgggagatcactgagaaaatactaaattcgtttgagtatacttcgagatttacaaaaacaaaaactttataccaattcctcttcctagctactttcatcaattgtggaagattcagcgatattaagaacgttgatccgaaatcatttaaattagtccaaaataagtatctgggagtaataatccagtgtttagtgacagagacaaagacaagcgttagtaggcacatatacttctttagcgcaaggggtaggatcgatccacttgtatatttggatgaatttttgaggaattctgaaccagtcctaaaacgagtaaataggaccggcaattcttcaagcaataaacaggaataccaattattaaaagataacttagtcagatcgtacaataaagctttgaagaaaaatgcgccttattcaatctttgctataaaaaatggcccaaaatctcacattggaagacatttgatgacctcatttctttcaatgaagggcctaacggagttgactaatgttgtgggaaattggagcgataagcgtgcttctgccgtggccaggacaacgtatactcatcagataacagcaatacctgatcactacttcgcactagtttctcggtactatgcatatgatccaatatcaaaggaaatgatagcattgaaggatgagactaatccaattgaggagtggcagcatatagaacagctaaagggtagtgctgaaggaagcatacgataccccgcatggaatgggataatatcacaggaggtactagactacctttcatcctacataaatagacgcatataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015002_sequence/1
type: Sequence
persistent identity: BBa K1015002 sequence
display id: BBa_K1015002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacttagccatttcaacaccttctttcaaatatttataataaactattgacaccgatattacaattgtaatattattgatttataaaaattacaactgtaatatcggagggtttatttatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataaatattctaaatgcataataaatactgataacatcttatattttgtattatattttgtattatcgttgacatgtataattttgatatcaaaaactgattttccctctattattttcgagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1015003_sequence/1
type: Sequence
persistent identity: BBa K1015003 sequence
display id: BBa_K1015003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacggtagccgctgacacaggtctgcggctggaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z