IRR/IRL(Ptet)-FLP-Sm(insert between lacA and cynR)
With acteriophage λ recombination sysytem, this device insert into Escherichia coli genome. This device produce FLP and AadA(streptomycin resistant gene) . But if site specific recombination be caused between IRR site and IRL site by FimE recombinase, pTet promoter change "OFF" state and FLP production stop.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
fimE inverted repeat right recombination site
fimE inverted repeat left recombination site
Primers were synthesized to bind to part Q04400, and then a PCR reaction was run.
Inverted tetR promoter with noncoding, spacer DNA downstream of it to make the total length 250 bp. The promoter faces the 5' direction.
This part is in a series of inverted tetR promoters that have total lengths of 150, 250, 350, 450, 650, and 850 bp. We chose the region upstream of tetR in part Q04400 to be the noncoding DNA segment. This noncoding segment consists of the 3' end of tetR and B0015. None of the parts in this series was long enough to include the start codon of tetR, so a functional tetR protein should not be transcribed.
lacA-homology sequence(for RED recombination system)
This sequence is 36bp homologous sequence with Escherichia coli lacA gene. Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and lacA gene. Because of the recombination, Biobrick part can insert into E.coli genome.
2??m plasmid of Saccharomyces cerevisiae.
site specific recombinase that recognize FRT site. FLP catalyze site specific recombination between two FRT site.
site specific recombination between two FRT site.
streptmycin resisitance gene (can PCR ampicillin resistance gene primer)
aminoglycoside resistance gene (aadA) come from R100.1 plasmid.
streptmycin resisitance gene(aadA) This aadA gene are inserted into blaZ gene come from pI258 plasmid. So this aadA gene can PCR with primers for blaZ.
cynR homology sequence(for RED recombination system)
This sequence is 36bp homologous sequence with Escherichia coli cynR gene. Bacteriophage λ recombination system(RED system) can cause homologous recombination between this sequence and cynR gene. Because of the recombination, Biobrick part can insert into E.coli genome.
Conversion of the iGEM parts registry to SBOL2.1