BBa_K1015005 1 BBa_K1015005 pBAD promoter 2013-09-14T11:00:00Z 2015-05-08T01:08:42Z plasmid pBAD Myc-HisA Arabinose-inducible araBAD promoter region and down stream sequence to the　initiation atg. false false _1321_ 0 15618 9 Not in stock false Arabinose-inducible promoter false Kento Tominaga annotation2343706 1 pBAD range2343706 1 1 249 BBa_K1015004 1 BBa_K1015004 Escherichia coli araC gene (for RED system) 2013-08-28T11:00:00Z 2015-05-08T01:08:42Z Escherichia coli Escherichia coli araC gene false false _1321_ 0 15618 9 Not in stock false For target site of homologous recombination with E.coli genome araC , using RED recombination system. false Kento Tominaga annotation2343705 1 araC range2343705 1 50 928 BBa_K1015015 1 BBa_K1015015 araC-pBAD-hbiF-Tc(genome insertion parts between araC and araA) 2013-09-15T11:00:00Z 2015-05-08T01:08:42Z BBa_K1015004 BBa_K1015005 BBa_K1015006 BBa_K880000 BBa_K1015007 BBa_K1015014 With bacteriophage λ recombination system, this device insert into Escherichia coli genome. This device produce hbiF recombinase and tetracycline resistance protein (tetA). HbiF production level are regulated by arabinose inducible promoter pBAD. false false _1321_ 0 15618 9 It's complicated true genome insertion device false Kento Tominaga component2346484 1 BBa_K1015006 component2346486 1 BBa_K1015007 component2346479 1 BBa_K1015004 component2346481 1 BBa_K1015005 component2346482 1 BBa_K880000 annotation2346482 1 BBa_K880000 range2346482 1 1604 2164 annotation2346479 1 BBa_K1015004 range2346479 1 1 1272 annotation2346484 1 BBa_K1015006 range2346484 1 2173 3747 annotation2346481 1 BBa_K1015005 range2346481 1 1281 1597 annotation2346486 1 BBa_K1015007 range2346486 1 3756 3795 BBa_K1015006 1 BBa_K1015006 TetA gene from E.coli 2013-09-15T11:00:00Z 2015-05-08T01:08:42Z Actinomycetes Tetracycline is a broad-spectrum polyketide antibiotic produced by the Streptomyces genus of Actinobacteria, indicated for use against many bacterial infections. It is a protein synthesis inhibitor. This part includes a gene which has resistance to tetracyclin. In our project, we use this part as a marker to select colonies of E.coli which succeeded in transformation. false false _1321_ 0 11941 9 Not in stock false This part can be used as a selection marker. false Eri Sakurai annotation2346332 1 tetA range2346332 1 144 1349 BBa_K880000 1 hbiF HbiF Recombinase (RFC25) 2012-09-28T11:00:00Z 2015-05-08T01:13:40Z Reference: Xie et al. Infect. Immun. 2006, 74(7):4039 -HbiF-RFC25 The Hbif recombinase is a putative regulator of the Escherichia coli type 1 fimbriation system, capable of switching the fimS invertible region in the production of fimbriae in a manner similar to the better characterized FimB and FimE recombinases. Orientation control of the fimS switch by recombinases is specific to directionality, with Hbif catalysing the conversion of ???OFF??? oriented regions to ???ON??? oriented ones such that the fimS promoter is facing genes responsible for type 1 fimbriae production. The hbiF gene was synthesized in vitro as device capable of opposing the directional inversion of the characterized fimE gene K137007 for the purpose of engineering bidirectional molecular switches. A synthetic circuit can be made by fusing two unique invertable repeats (IRs) recognized by the recombinase-parts K137008 and K137010, in that order- flanking a sequence of interest of 200-300bp and expressing hbiF or fimE in low-to-moderate levels to cause the sequence to invert: fimE will cause a template strand between the IRs to face the upstream coding strand. hbiF will cause a coding strand between the IRs to face the downstream template strand. Note that IRs??? initial orientation is FimE flippable, but not HbiF flippable. This reaction can be characterized by digesting asymmetrical digest reporter K880001 with EcoRI and AgeI and determining the lengths of the resulting fragments. Additionally, HbiF contains an RFC25 suffix and is capable of undergoing protein fusions to fluorescent proteins for quantification, affinity purification tags, or degradation tags such as K880004 to increase circuit responsiveness. Reference: Xie et al. Infect. Immun. 2006, 74(7):4039 false false _1142_ 0 9403 9 In stock true This part contains an RFC25 suffix and is capable of undergoing protein fusions to fluorescent proteins for quantification, affinity purification tags, or degradation tags such as K880004 to increase circuit responsiveness. false Josh Atkinson, Mike Ferguson, and Ben Parker BBa_K1015007 1 BBa_K1015007 araC homology sequence (for RED recombination system) 2013-09-15T11:00:00Z 2015-05-08T01:08:42Z Escherichia coli MG1655 araC gene This sequence is homologous sequence with Escherichia coli araC gene. Bacterio phage λ recombination system(RED system) can cause homologous recombination between this sequence and araC gene. Because of the recombination, Biobrick part can be inserted into E. coli genome. false false _1321_ 0 15618 9 Not in stock false Genome insertion false Kento Tominaga annotation2346364 1 araA-homology sequence range2346364 1 1 40 BBa_K880000_sequence 1 atgacgagaaaatatctcacacaggatgaagtctacaggctgatggatgcagcccagagcatgtcctttcctgaaagaaatcgctgtctgattatgatggctttcattcacggttttagggccagtgaacttcttgatttacgtttatccgatattgatgcatctggaaaacaacttaatattcggcgtattaaaaatggtttctcgacaacacatcctcttcttcctgatgaatataatttgatcaagttgtggcttaagcagaggaagttaattgaaaacggagttgaaggagactggctttttctctcccggaaacgccgccctatcagcagacaacattttttttctatcattcgtgaggctggaaaacgtgcaggattagctgtaaaagcacatcctcatatgttacgtcatgcctgtggttttgctctggctgacaatggtgtcgatacccgactacttcaggattacctgggtcatcgaaatattcagcatactgtcagatacacagccagtaatgctgctcgttttaaaggggtgtggaaaaaaaagcctcgt BBa_K1015015_sequence 1 aaaccctatgctactccgtcaagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaacaggaggaattaacctactagagagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaacaggaggaattaacctactagatgacgagaaaatatctcacacaggatgaagtctacaggctgatggatgcagcccagagcatgtcctttcctgaaagaaatcgctgtctgattatgatggctttcattcacggttttagggccagtgaacttcttgatttacgtttatccgatattgatgcatctggaaaacaacttaatattcggcgtattaaaaatggtttctcgacaacacatcctcttcttcctgatgaatataatttgatcaagttgtggcttaagcagaggaagttaattgaaaacggagttgaaggagactggctttttctctcccggaaacgccgccctatcagcagacaacattttttttctatcattcgtgaggctggaaaacgtgcaggattagctgtaaaagcacatcctcatatgttacgtcatgcctgtggttttgctctggctgacaatggtgtcgatacccgactacttcaggattacctgggtcatcgaaatattcagcatactgtcagatacacagccagtaatgctgctcgttttaaaggggtgtggaaaaaaaagcctcgttactagagccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctagacatcattaattcctaatttttgttgacactctatcattgatagagttattttaccactccctatcagtgatagagaaaagtgaaatgaatagttcgacaaagatcgcattggtaattacgttactcgatgccatggggattggccttatcatgccagtcttgccaacgttattacgtgaatttattgcttcggaagatatcgctaaccactttggcgtattgcttgcactttatgcgttaatgcaggttatctttgctccttggcttggaaaaatgtctgaccgatttggtcggcgcccagtgctgttgttgtcattaataggcgcatcgctggattacttattgctggctttttcaagtgcgctttggatgctgtatttaggccgtttgctttcagggatcacaggagctactggggctgtcgcggcatcggtcattgccgataccacctcagcttctcaacgcgtgaagtggttcggttggttaggggcaagttttgggcttggtttaatagcggggcctattattggtggttttgcaggagagatttcaccgcatagtcccttttttatcgctgcgttgctaaatattgtcactttccttgtggttatgttttggttccgtgaaaccaaaaatacacgtgataatacagataccgaagtaggggttgagacgcaatcgaattcggtatacatcactttatttaaaacgatgcccattttgttgattatttatttttcagcgcaattgataggccaaattcccgcaacggtgtgggtgctatttaccgaaaatcgttttggatggaatagcatgatggttggcttttcattagcgggtcttggtcttttacactcagtattccaagcctttgtggcaggaagaatagccactaaatggggcgaaaaaacggcagtactgctcgaatttattgcagatagtagtgcatttgcctttttagcgtttatatctgaaggttggttagatttccctgttttaattttattggctggtggtgggatcgctttacctgcattacagggagtgatgtctatccaaacaaagagtcatgagcaaggtgctttacagggattattggtgagccttaccaatgcaaccggtgttattggcccattactgtttactgttatttataatcattcactaccaatttgggatggctggatttggattattggtttagcgttttactgtattattatcctgctatcgatgaccttcatgttaacccctcaagctcaggggagtaaacaggagacaagtgcttagttatttcgtcaccaaatgatgttattccgcgaaatataatgaccctcttgataacccaagagggcattttttacgataaagaagatttagcttcaaataaaacctatctattttatttatctttcaagctcaataaaaagccgcggtaaatagcaataaattggccttttttatcggcaagctcttttaggtttttcgcatgtattgcgatatgcataaaccagcctactagagcgtcgctaagtagccgcatccggtatgtaacgcctgatgc BBa_K1015007_sequence 1 cgtcgctaagtagccgcatccggtatgtaacgcctgatgc BBa_K1015006_sequence 1 ccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctagacatcattaattcctaatttttgttgacactctatcattgatagagttattttaccactccctatcagtgatagagaaaagtgaaatgaatagttcgacaaagatcgcattggtaattacgttactcgatgccatggggattggccttatcatgccagtcttgccaacgttattacgtgaatttattgcttcggaagatatcgctaaccactttggcgtattgcttgcactttatgcgttaatgcaggttatctttgctccttggcttggaaaaatgtctgaccgatttggtcggcgcccagtgctgttgttgtcattaataggcgcatcgctggattacttattgctggctttttcaagtgcgctttggatgctgtatttaggccgtttgctttcagggatcacaggagctactggggctgtcgcggcatcggtcattgccgataccacctcagcttctcaacgcgtgaagtggttcggttggttaggggcaagttttgggcttggtttaatagcggggcctattattggtggttttgcaggagagatttcaccgcatagtcccttttttatcgctgcgttgctaaatattgtcactttccttgtggttatgttttggttccgtgaaaccaaaaatacacgtgataatacagataccgaagtaggggttgagacgcaatcgaattcggtatacatcactttatttaaaacgatgcccattttgttgattatttatttttcagcgcaattgataggccaaattcccgcaacggtgtgggtgctatttaccgaaaatcgttttggatggaatagcatgatggttggcttttcattagcgggtcttggtcttttacactcagtattccaagcctttgtggcaggaagaatagccactaaatggggcgaaaaaacggcagtactgctcgaatttattgcagatagtagtgcatttgcctttttagcgtttatatctgaaggttggttagatttccctgttttaattttattggctggtggtgggatcgctttacctgcattacagggagtgatgtctatccaaacaaagagtcatgagcaaggtgctttacagggattattggtgagccttaccaatgcaaccggtgttattggcccattactgtttactgttatttataatcattcactaccaatttgggatggctggatttggattattggtttagcgttttactgtattattatcctgctatcgatgaccttcatgttaacccctcaagctcaggggagtaaacaggagacaagtgcttagttatttcgtcaccaaatgatgttattccgcgaaatataatgaccctcttgataacccaagagggcattttttacgataaagaagatttagcttcaaataaaacctatctattttatttatctttcaagctcaataaaaagccgcggtaaatagcaataaattggccttttttatcggcaagctcttttaggtttttcgcatgtattgcgatatgcataaaccagcc BBa_K1015004_sequence 1 aaaccctatgctactccgtcaagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaacaggaggaattaacc BBa_K1015005_sequence 1 agaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgttttttgggctaacaggaggaattaacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z