LOCUS BBa_K1017101 1617 bp DNA linear 22-Nov-2024 DEFINITION Pred ( PompC + B0032 + LacI + J61048 + Plac) ACCESSION BBa_K1017101 VERSION BBa_K1017101.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 RBS 117..129 /label=RBS-3\Weak RBS 123..126 /label=RBS misc_feature 117..129 /label=BBa_B0032 RBS 117..129 /label=BBa_B0032 misc_feature 1225..1263 /label=LVA CDS 136..1263 /label=lacI-LVA misc_feature 136..1263 /label=BBa_C0012 misc_feature 1264..1288 /label=Help:Barcodes CDS 136..1263 /label=BBa_C0012 terminator 1297..1409 /label=BBa_J61048 CDS 1418..1505 /label=end of LacI coding region (inactive) misc_feature 1418..1617 /label=BBa_R0010 misc_feature 1506..1543 /label=CAP binding site promoter 1554..1559 /label=-35 promoter 1578..1583 /label=-10 misc_feature 1583..1617 /label=LacI binding site misc_feature 1590..1590 /label=start promoter 1418..1617 /label=BBa_R0010 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagtcac 121 acaggaaagt actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 181 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 241 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 301 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 361 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 421 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 481 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 541 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 601 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 661 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 721 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 781 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 841 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 901 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 961 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 1021 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 1081 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 1141 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 1201 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 1261 taactctgat agtgctagtg tagatctcta ctagagccgg cttatcggtc agtttcacct 1321 gatttacgta aaaacccgct tcggcgggtt tttgcttttg gaggggcaga aagatgaatg 1381 actgtccacg acgctatacc caaaagaaat actagagcaa tacgcaaacc gcctctcccc 1441 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 1501 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac 1561 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacaca //