LOCUS BBa_K1019008 5494 bp DNA linear 09-Dec-2025 DEFINITION pEBS Expression Plasmid ACCESSION BBa_K1019008 VERSION BBa_K1019008.1 FEATURES Location/Qualifiers misc_feature 3716..4398 /label=ColE1 origin misc_feature 3358..3664 /label=F1 origin misc_feature 3226..3681 /label=M13 origin CDS 2464..3123 /label=Amp Resistance CDS 1672..1740 /label=LacZ alpha misc_feature 1438..1460 /label=LacO RBS 1549..1560 /label=RBS primer_bind 1599..1616 /label=M13F misc_feature 1561..1563 /label=Start CDS 81..1163 /label=LacI misc_feature 1941..2020 /label=rrnB T1 terminator misc_feature 2127..2155 /label=rrnB T2 terminator misc_feature 1564..1569 /label=2x ochre STOP misc_feature 1529..1548 /label=Prefix misc_feature 1571..1590 /label=suffix primer_bind 1450..1469 /label=M13R ORIGIN 1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1501 gcacttcacc aacaaggacc atagcataga attcgcggcc gcttctagag gaggtaaaaa 1561 atgtaataat actagtagcg gccgctgcag agcttggcac tggccgtcgt tttacaacgt 1621 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttttcgccag ctggcgtaat 1681 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 1741 cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat taaatcagaa 1801 cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct 1861 gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc 1921 catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg 1981 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc 2041 gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc 2101 ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt 2161 tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 2221 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat 2281 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca 2341 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc 2401 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttctcca 2461 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg 2521 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca 2581 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata 2641 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag 2701 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg 2761 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca 2821 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta 2881 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct 2941 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca 3001 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag 3061 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat 3121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttacc ccggttgata 3181 atcagaaaag ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaaacgtta 3241 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg 3301 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 3361 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 3421 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg 3481 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 3541 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 3601 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 3661 atgcgccgct acagggcgcg taaaaggatc taggtgaaga tcctttttga taatctcatg 3721 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 3781 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 3841 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 3901 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 3961 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4021 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4081 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4141 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4201 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 4261 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 4321 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 4381 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 4441 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 4501 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 4561 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat 4621 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct 4681 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc 4741 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag 4801 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag 4861 ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc 4921 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc 4981 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt 5041 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc 5101 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag 5161 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg 5221 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg 5281 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc 5341 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta 5401 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg 5461 cacccgtggc caggacccaa cgctgcccga aatt //