LOCUS       BBa_K1020005            2146 bp    DNA     linear       10-May-2024
DEFINITION  Alkane Marker--Device for detection of various alkanes
ACCESSION   BBa_K1020005
VERSION     BBa_K1020005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..178
                     /label=alkM promoter
     CDS             198..879
                     /label=RFP
     misc_feature    885..1013
                     /label=terminator
     misc_feature    1..1013
                     /label=BBa_K1020001
     promoter        1022..1056
                     /label=BBa_J23103
     misc_feature    1069..1072
                     /label=conserved
     RBS             1065..1076
                     /label=BBa_B0034
     misc_feature    1083..2009
                     /label=AlkR
     CDS             1083..2009
                     /label=BBa_K1020002
     stem_loop       2029..2072
                     /label=stem_loop
     misc_feature    2018..2097
                     /label=BBa_B0010
     terminator      2018..2097
                     /label=BBa_B0010
     stem_loop       2113..2132
                     /label=T7 TE
     misc_feature    2139..2139
                     /label=stop
     polyA_site      2133..2146
                     /label=polya
     misc_feature    2106..2146
                     /label=BBa_B0012
     terminator      2106..2146
                     /label=BBa_B0012
     terminator      2018..2146
                     /label=BBa_B0015
ORIGIN
        1 agcgcgttaa aaattcttat ttgagcaaga gtatagaatg aggttgtagc ttatacatgt
       61 gattttagga cattgacatg gttgaaaatg ccaaagactt tgacataggt tttttggacg
      121 ataacgactg tttttaatat ataagatgga tgattatgag cagtatgaat agaggataga
      181 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      241 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
      301 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
      361 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
      421 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
      481 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
      541 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
      601 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
      661 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
      721 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
      781 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
      841 aacgtgctga aggtcgtcac tccaccggtg cttaataaac tagaccaggc atcaaataaa
      901 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      961 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     1021 gctgatagct agctcagtcc tagggattat gctagctact agagaaagag gagaaatact
     1081 agatggatgc acttagtaaa atttttgatg atattcattt aaataaatct gaatatctct
     1141 atgtaaaaac tcaaggagaa tgggcatttc atatgcttgc ccaagatgct ttgattgctc
     1201 acattatttt aatgggttct gctcatttca cattacagga tggtacgaca caaaccgctt
     1261 attcgggaga tattgtgctt attccttctg ggcaagctca ttttgtctca aatgatgctc
     1321 agaacaaact aattgattgt tccaacattc agtctttttt tgatggacac cgcaatgatg
     1381 ccattgagtt aggcacaaca tcttcggatc atggtttaat ttttactgtg cggagccata
     1441 tcgatatgca tatgggtagc cctttactca atgctttacc atcactgata catattcatc
     1501 acgcaatgag ctcaactggc ccagagtggt tacgcgtcgg tttatatttt gtggcacttg
     1561 aaacccagcg tattcaacca ggacgagata aaatatttga ccacttgatg agcatcctat
     1621 ttatagaatg tgtccgcgat catattgccc agcttgatga ccccaaaagc tggttaactg
     1681 cactcatgca tcccgaatta tctaatgcgc tcgcagctat tcatgcttac cccgaaaaac
     1741 cttggactgt agagagtttg gcagatcaat gttgcatgtc acggtctaaa tttgccacac
     1801 tttttcaaag tattgttcat gagacccctc tcgcttatct tcaacaacat cgccttcgtc
     1861 tggccgtaca gttattaaaa accagtcagt taaatattca gcaaattgcc aataaagtcg
     1921 gatactcctc agaaacagct tttagtcagg catttaaacg tcaatttgaa caatctccta
     1981 aacactatcg ccagcaatca tgataataat actagagcca ggcatcaaat aaaacgaaag
     2041 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2101 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//