LOCUS       BBa_K1020012            2662 bp    DNA     linear       07-May-2024
DEFINITION  Alkane Selector for intracellular alkane production
ACCESSION   BBa_K1020012
VERSION     BBa_K1020012.1  
FEATURES             Location/Qualifiers
     promoter        1..178
                     /label=PalkM promoter
     misc_feature    203..1394
                     /label=tetA
     misc_feature    1400..1529
                     /label=terminator
     CDS             1..1529
                     /label=BBa_K1020010
     promoter        1538..1572
                     /label=BBa_J23103
     misc_feature    1585..1588
                     /label=conserved
     RBS             1581..1592
                     /label=BBa_B0034
     misc_feature    1599..2525
                     /label=AlkR
     CDS             1599..2525
                     /label=BBa_K1020002
     stem_loop       2545..2588
                     /label=stem_loop
     misc_feature    2534..2613
                     /label=BBa_B0010
     terminator      2534..2613
                     /label=BBa_B0010
     stem_loop       2629..2648
                     /label=T7 TE
     misc_feature    2655..2655
                     /label=stop
     polyA_site      2649..2662
                     /label=polya
     misc_feature    2622..2662
                     /label=BBa_B0012
     terminator      2622..2662
                     /label=BBa_B0012
     terminator      2534..2662
                     /label=BBa_B0015
     misc_feature    1538..2662
                     /label=BBa_K1020003
ORIGIN
        1 agcgcgttaa aaattcttat ttgagcaaga gtatagaatg aggttgtagc ttatacatgt
       61 gattttagga cattgacatg gttgaaaatg ccaaagactt tgacataggt tttttggacg
      121 ataacgactg tttttaatat ataagatgga tgattatgag cagtatgaat agaggataac
      181 taggaaagag gagaaatact agatgaaatc taacaatgcg ctcatcgtca tcctcggcac
      241 cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
      301 ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
      361 gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
      421 cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
      481 acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
      541 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
      601 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
      661 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
      721 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc
      781 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
      841 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
      901 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
      961 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     1021 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     1081 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     1141 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     1201 gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct
     1261 gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     1321 aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     1381 cacctcgacc taaactagac caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1441 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1501 ccttcgggtg ggcctttctg cgtttatatt actagagctg atagctagct cagtcctagg
     1561 gattatgcta gctactagag aaagaggaga aatactagat ggatgcactt agtaaaattt
     1621 ttgatgatat tcatttaaat aaatctgaat atctctatgt aaaaactcaa ggagaatggg
     1681 catttcatat gcttgcccaa gatgctttga ttgctcacat tattttaatg ggttctgctc
     1741 atttcacatt acaggatggt acgacacaaa ccgcttattc gggagatatt gtgcttattc
     1801 cttctgggca agctcatttt gtctcaaatg atgctcagaa caaactaatt gattgttcca
     1861 acattcagtc tttttttgat ggacaccgca atgatgccat tgagttaggc acaacatctt
     1921 cggatcatgg tttaattttt actgtgcgga gccatatcga tatgcatatg ggtagccctt
     1981 tactcaatgc tttaccatca ctgatacata ttcatcacgc aatgagctca actggcccag
     2041 agtggttacg cgtcggttta tattttgtgg cacttgaaac ccagcgtatt caaccaggac
     2101 gagataaaat atttgaccac ttgatgagca tcctatttat agaatgtgtc cgcgatcata
     2161 ttgcccagct tgatgacccc aaaagctggt taactgcact catgcatccc gaattatcta
     2221 atgcgctcgc agctattcat gcttaccccg aaaaaccttg gactgtagag agtttggcag
     2281 atcaatgttg catgtcacgg tctaaatttg ccacactttt tcaaagtatt gttcatgaga
     2341 cccctctcgc ttatcttcaa caacatcgcc ttcgtctggc cgtacagtta ttaaaaacca
     2401 gtcagttaaa tattcagcaa attgccaata aagtcggata ctcctcagaa acagctttta
     2461 gtcaggcatt taaacgtcaa tttgaacaat ctcctaaaca ctatcgccag caatcatgat
     2521 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2581 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     2641 ggtgggcctt tctgcgttta ta
//